Study on extraction process of active ingredients with hypouricuria effect from Plantaginis Herba(车前草降血尿酸有效成分提取工艺的研究)
建立小鼠急性高尿酸血症模型,通过灌胃给药考察车前草的降血尿酸作用.采用正交试验设计,以车前草所含大车前苷为指标,考察乙醇浓度、提取时间、提取次数和乙醇用量4种因素对提取结果的影响.大车前苷和血尿酸的含量均用HPLC法测定.结果表明,车前草醇提组的小鼠血清尿酸水平明显低于模型组(P<0.05).乙醇浓度、提取时间、提取次数和乙醇用量4种因素对车前草乙醇提取物中的大车前苷含量均有显著影响.最佳提取工艺为用10倍药材量的60%乙醇提取2次,每次2 h....
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2010-09-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/10.3785/j.issn.1008-9497.2010.05.018 |
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| author | QIANYing(钱莺) FUXu-chun(傅旭春) WANGJian-ping(王建平) BAIHai-bo(白海波) |
| author_facet | QIANYing(钱莺) FUXu-chun(傅旭春) WANGJian-ping(王建平) BAIHai-bo(白海波) |
| author_sort | QIANYing(钱莺) |
| collection | DOAJ |
| description | 建立小鼠急性高尿酸血症模型,通过灌胃给药考察车前草的降血尿酸作用.采用正交试验设计,以车前草所含大车前苷为指标,考察乙醇浓度、提取时间、提取次数和乙醇用量4种因素对提取结果的影响.大车前苷和血尿酸的含量均用HPLC法测定.结果表明,车前草醇提组的小鼠血清尿酸水平明显低于模型组(P<0.05).乙醇浓度、提取时间、提取次数和乙醇用量4种因素对车前草乙醇提取物中的大车前苷含量均有显著影响.最佳提取工艺为用10倍药材量的60%乙醇提取2次,每次2 h. |
| first_indexed | 2024-04-24T16:59:39Z |
| format | Article |
| id | doaj.art-b0a191457f0241689ae0e2d552e3dbc5 |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T16:59:39Z |
| publishDate | 2010-09-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-b0a191457f0241689ae0e2d552e3dbc52024-03-29T01:58:28ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972010-09-0137556056210.3785/j.issn.1008-9497.2010.05.018Study on extraction process of active ingredients with hypouricuria effect from Plantaginis Herba(车前草降血尿酸有效成分提取工艺的研究)QIANYing(钱莺)0FUXu-chun(傅旭春)1WANGJian-ping(王建平)2BAIHai-bo(白海波)3 1.Pharmaceutical College of Zhejiang University, Hangzhou 310058, China( 1.浙江大学药学院，浙江 杭州 310058) 2.Department of Pharmacy, City College of Zhejiang University, Hangzhou 310015, China( 2.浙江大学城市学院药学系，浙江 杭州 310015) 3.Zhejiang Traditional Chinese Medical Hospital, Hangzhou 310006, China( 3.浙江省中医院，浙江 杭州 310006) 4.Hangzhou East China Pharmaceutical Bioengineer Institute Company, Hangzhou 310012, China( 4.杭州华东医药集团生物工程研究所有限公司，浙江 杭州 310012)建立小鼠急性高尿酸血症模型,通过灌胃给药考察车前草的降血尿酸作用.采用正交试验设计,以车前草所含大车前苷为指标,考察乙醇浓度、提取时间、提取次数和乙醇用量4种因素对提取结果的影响.大车前苷和血尿酸的含量均用HPLC法测定.结果表明,车前草醇提组的小鼠血清尿酸水平明显低于模型组(P<0.05).乙醇浓度、提取时间、提取次数和乙醇用量4种因素对车前草乙醇提取物中的大车前苷含量均有显著影响.最佳提取工艺为用10倍药材量的60%乙醇提取2次,每次2 h.https://doi.org/10.3785/j.issn.1008-9497.2010.05.018车前草大车前苷血尿酸提取工艺正交试验设计 |
| spellingShingle | QIANYing(钱莺) FUXu-chun(傅旭春) WANGJian-ping(王建平) BAIHai-bo(白海波) Study on extraction process of active ingredients with hypouricuria effect from Plantaginis Herba(车前草降血尿酸有效成分提取工艺的研究) Zhejiang Daxue xuebao. Lixue ban 车前草 大车前苷 血尿酸 提取工艺 正交试验设计 |
| title | Study on extraction process of active ingredients with hypouricuria effect from Plantaginis Herba(车前草降血尿酸有效成分提取工艺的研究) |
| title_full | Study on extraction process of active ingredients with hypouricuria effect from Plantaginis Herba(车前草降血尿酸有效成分提取工艺的研究) |
| title_fullStr | Study on extraction process of active ingredients with hypouricuria effect from Plantaginis Herba(车前草降血尿酸有效成分提取工艺的研究) |
| title_full_unstemmed | Study on extraction process of active ingredients with hypouricuria effect from Plantaginis Herba(车前草降血尿酸有效成分提取工艺的研究) |
| title_short | Study on extraction process of active ingredients with hypouricuria effect from Plantaginis Herba(车前草降血尿酸有效成分提取工艺的研究) |
| title_sort | study on extraction process of active ingredients with hypouricuria effect from plantaginis herba 车前草降血尿酸有效成分提取工艺的研究 |
| topic | 车前草 大车前苷 血尿酸 提取工艺 正交试验设计 |
| url | https://doi.org/10.3785/j.issn.1008-9497.2010.05.018 |
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