Synergistic Effects of Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) Combination in Angiogenesis Behavior in EAhy 926 Cells
Background: This study investigated the combination effects of the Danshen and Sanqi herb pair on angiogenesis in vitro. Methods: Nine combination ratios of Danshen-Sanqi extracts (DS-SQ) were screened for their angiogenic effects in the human vascular endothelial EAhy 926 cell line via cell prolife...
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MDPI AG
2017-11-01
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| Series: | Medicines |
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| Online Access: | https://www.mdpi.com/2305-6320/4/4/85 |
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| author | Xian Zhou Valentina Razmovski-Naumovski Antony Kam Dennis Chang Chunguang Li Alan Bensoussan Kelvin Chan |
| author_facet | Xian Zhou Valentina Razmovski-Naumovski Antony Kam Dennis Chang Chunguang Li Alan Bensoussan Kelvin Chan |
| author_sort | Xian Zhou |
| collection | DOAJ |
| description | Background: This study investigated the combination effects of the Danshen and Sanqi herb pair on angiogenesis in vitro. Methods: Nine combination ratios of Danshen-Sanqi extracts (DS-SQ) were screened for their angiogenic effects in the human vascular endothelial EAhy 926 cell line via cell proliferation, cell migration and tube formation activities against the damage to the cells exerted by DL-homocysteine (Hcy) and adenosine (Ado). The type of interaction (synergistic, antagonistic, additive) between Danshen and Sanqi was analyzed using combination index (CI) and isobologram models. The angiogenic activities of key bioactive compounds from Danshen and Sanqi were tested in the same models. Results: DS-SQ ratios of 2:8 and 3:7 (50–300 µg/mL) potentiated angiogenic synergistic effects (CI < 1) in all three assays. The observed wound healing effects of DS-SQ 2:8 was significantly attenuated by phosphatidylinositol-3 kinases (PI3K), mitogen-activated protein kinase (MEK) and extracellular signal-regulated kinases (ERK) inhibitors which inferred the potential mechanistic pathways. Out of all the tested compounds, Notoginsenoside R1 from Sanqi exhibited the most potent bioactivity in cell proliferation assay. Conclusions: This study provides scientific evidence to support the traditional use of the Danshen-Sanqi combination for vascular disease, in particular through their synergistic interactions on previously unexamined angiogenic pathways. |
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| issn | 2305-6320 |
| language | English |
| last_indexed | 2024-12-20T12:39:47Z |
| publishDate | 2017-11-01 |
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| spelling | doaj.art-b0a4c18a23eb473aaa2b3d627bba30862022-12-21T19:40:30ZengMDPI AGMedicines2305-63202017-11-01448510.3390/medicines4040085medicines4040085Synergistic Effects of Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) Combination in Angiogenesis Behavior in EAhy 926 CellsXian Zhou0Valentina Razmovski-Naumovski1Antony Kam2Dennis Chang3Chunguang Li4Alan Bensoussan5Kelvin Chan6National Institute of Complementary Medicine (NICM), Western Sydney University, Penrith, NSW 2751, AustraliaNational Institute of Complementary Medicine (NICM), Western Sydney University, Penrith, NSW 2751, AustraliaNational Institute of Complementary Medicine (NICM), Western Sydney University, Penrith, NSW 2751, AustraliaNational Institute of Complementary Medicine (NICM), Western Sydney University, Penrith, NSW 2751, AustraliaNational Institute of Complementary Medicine (NICM), Western Sydney University, Penrith, NSW 2751, AustraliaNational Institute of Complementary Medicine (NICM), Western Sydney University, Penrith, NSW 2751, AustraliaNational Institute of Complementary Medicine (NICM), Western Sydney University, Penrith, NSW 2751, AustraliaBackground: This study investigated the combination effects of the Danshen and Sanqi herb pair on angiogenesis in vitro. Methods: Nine combination ratios of Danshen-Sanqi extracts (DS-SQ) were screened for their angiogenic effects in the human vascular endothelial EAhy 926 cell line via cell proliferation, cell migration and tube formation activities against the damage to the cells exerted by DL-homocysteine (Hcy) and adenosine (Ado). The type of interaction (synergistic, antagonistic, additive) between Danshen and Sanqi was analyzed using combination index (CI) and isobologram models. The angiogenic activities of key bioactive compounds from Danshen and Sanqi were tested in the same models. Results: DS-SQ ratios of 2:8 and 3:7 (50–300 µg/mL) potentiated angiogenic synergistic effects (CI < 1) in all three assays. The observed wound healing effects of DS-SQ 2:8 was significantly attenuated by phosphatidylinositol-3 kinases (PI3K), mitogen-activated protein kinase (MEK) and extracellular signal-regulated kinases (ERK) inhibitors which inferred the potential mechanistic pathways. Out of all the tested compounds, Notoginsenoside R1 from Sanqi exhibited the most potent bioactivity in cell proliferation assay. Conclusions: This study provides scientific evidence to support the traditional use of the Danshen-Sanqi combination for vascular disease, in particular through their synergistic interactions on previously unexamined angiogenic pathways.https://www.mdpi.com/2305-6320/4/4/85Danshen-Sanqisynergyangiogenesis |
| spellingShingle | Xian Zhou Valentina Razmovski-Naumovski Antony Kam Dennis Chang Chunguang Li Alan Bensoussan Kelvin Chan Synergistic Effects of Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) Combination in Angiogenesis Behavior in EAhy 926 Cells Medicines Danshen-Sanqi synergy angiogenesis |
| title | Synergistic Effects of Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) Combination in Angiogenesis Behavior in EAhy 926 Cells |
| title_full | Synergistic Effects of Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) Combination in Angiogenesis Behavior in EAhy 926 Cells |
| title_fullStr | Synergistic Effects of Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) Combination in Angiogenesis Behavior in EAhy 926 Cells |
| title_full_unstemmed | Synergistic Effects of Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) Combination in Angiogenesis Behavior in EAhy 926 Cells |
| title_short | Synergistic Effects of Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) Combination in Angiogenesis Behavior in EAhy 926 Cells |
| title_sort | synergistic effects of danshen salvia miltiorrhizae radix et rhizoma and sanqi notoginseng radix et rhizoma combination in angiogenesis behavior in eahy 926 cells |
| topic | Danshen-Sanqi synergy angiogenesis |
| url | https://www.mdpi.com/2305-6320/4/4/85 |
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