A new method for preparing a rat intracerebral hemorrhage model by combining focused ultrasound and microbubbles
Abstract Background We aimed to prepare a non‐invasive, reproducible, and controllable rat model of intracerebral hemorrhage with focused ultrasound (FUS). Methods A rat intracerebral hemorrhage (ICH) model was established by combining FUS and microbubbles (μBs), and edaravone was used to verify whe...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-04-01
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| Series: | Animal Models and Experimental Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ame2.12303 |
| _version_ | 1827953985686863872 |
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| author | Yao He Jie Yang Fengying Hu Min Liao Yuru Nie Xiaoxia Zhu Tao Zhang Keer Song Qinxi Li Xiaojie Li Chenghan Mei Zhe Wu Qiang Lu Zhihui Zhong |
| author_facet | Yao He Jie Yang Fengying Hu Min Liao Yuru Nie Xiaoxia Zhu Tao Zhang Keer Song Qinxi Li Xiaojie Li Chenghan Mei Zhe Wu Qiang Lu Zhihui Zhong |
| author_sort | Yao He |
| collection | DOAJ |
| description | Abstract Background We aimed to prepare a non‐invasive, reproducible, and controllable rat model of intracerebral hemorrhage with focused ultrasound (FUS). Methods A rat intracerebral hemorrhage (ICH) model was established by combining FUS and microbubbles (μBs), and edaravone was used to verify whether the free radical scavenger had a protective effect on the model. The brain tissue of each group was sectioned to observe the gross histology, blood–brain barrier (BBB) permeability, cerebral infarction volume, and histopathological changes. Results Compared with the FUS group, the BBB permeability was significantly increased in the FUS + μBs (F&B) group (p = 0.0021). The second coronal slice in the F&B group had an obvious hemorrhage lesion, and the FUS + μBs + edaravone (F&B&E) group had smaller hemorrhage areas; however, ICH did not occur in the FUS group. The cerebral infarction volume in the F&B group was significantly larger than that in the FUS group (p = 0.0030) and F&B&E group (p = 0.0208). HE staining results showed that nerve fibrinolysis, neuronal necrosis, microglia production, and erythrocytes were found in both the F&B group and the F&B&E group, but the areas of the nerve fibrinolysis and neuronal necrosis in the F&B group were larger than the F&B&E group. Conclusions A rat ICH model was successfully prepared using the μBs assisted FUS treatment, and edaravone had a therapeutic effect on this model. This model can be used to study the pathophysiological mechanism of ICH‐related diseases and in preclinical research on related new drugs. |
| first_indexed | 2024-04-09T14:22:03Z |
| format | Article |
| id | doaj.art-b0a7c3d9598347129ce798e82aa3e54d |
| institution | Directory Open Access Journal |
| issn | 2576-2095 |
| language | English |
| last_indexed | 2024-04-09T14:22:03Z |
| publishDate | 2023-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Animal Models and Experimental Medicine |
| spelling | doaj.art-b0a7c3d9598347129ce798e82aa3e54d2023-05-04T16:07:59ZengWileyAnimal Models and Experimental Medicine2576-20952023-04-016210311010.1002/ame2.12303A new method for preparing a rat intracerebral hemorrhage model by combining focused ultrasound and microbubblesYao He0Jie Yang1Fengying Hu2Min Liao3Yuru Nie4Xiaoxia Zhu5Tao Zhang6Keer Song7Qinxi Li8Xiaojie Li9Chenghan Mei10Zhe Wu11Qiang Lu12Zhihui Zhong13Laboratory of Nonhuman Primate Disease Modeling Research, State Key Laboratory of Biotherapy, West China Hospital Sichuan University Chengdu ChinaDepartment of Medical Ultrasound, West China Hospital Sichuan University Chengdu ChinaSchool of Life Science and Technology University of Electronic Science and Technology of China Chengdu ChinaDepartment of Medical Ultrasound, West China Hospital Sichuan University Chengdu ChinaSchool of Life Science and Technology University of Electronic Science and Technology of China Chengdu ChinaDepartment of Medical Ultrasound, West China Hospital Sichuan University Chengdu ChinaSchool of Bioscience and Technology Chengdu Medical College Chengdu ChinaFranklin College of Arts and Science University of Georgia Athens Georgia USALaboratory of Nonhuman Primate Disease Modeling Research, State Key Laboratory of Biotherapy, West China Hospital Sichuan University Chengdu ChinaLaboratory of Nonhuman Primate Disease Modeling Research, State Key Laboratory of Biotherapy, West China Hospital Sichuan University Chengdu ChinaLaboratory of Nonhuman Primate Disease Modeling Research, State Key Laboratory of Biotherapy, West China Hospital Sichuan University Chengdu ChinaSchool of Life Science and Technology University of Electronic Science and Technology of China Chengdu ChinaDepartment of Medical Ultrasound, West China Hospital Sichuan University Chengdu ChinaLaboratory of Nonhuman Primate Disease Modeling Research, State Key Laboratory of Biotherapy, West China Hospital Sichuan University Chengdu ChinaAbstract Background We aimed to prepare a non‐invasive, reproducible, and controllable rat model of intracerebral hemorrhage with focused ultrasound (FUS). Methods A rat intracerebral hemorrhage (ICH) model was established by combining FUS and microbubbles (μBs), and edaravone was used to verify whether the free radical scavenger had a protective effect on the model. The brain tissue of each group was sectioned to observe the gross histology, blood–brain barrier (BBB) permeability, cerebral infarction volume, and histopathological changes. Results Compared with the FUS group, the BBB permeability was significantly increased in the FUS + μBs (F&B) group (p = 0.0021). The second coronal slice in the F&B group had an obvious hemorrhage lesion, and the FUS + μBs + edaravone (F&B&E) group had smaller hemorrhage areas; however, ICH did not occur in the FUS group. The cerebral infarction volume in the F&B group was significantly larger than that in the FUS group (p = 0.0030) and F&B&E group (p = 0.0208). HE staining results showed that nerve fibrinolysis, neuronal necrosis, microglia production, and erythrocytes were found in both the F&B group and the F&B&E group, but the areas of the nerve fibrinolysis and neuronal necrosis in the F&B group were larger than the F&B&E group. Conclusions A rat ICH model was successfully prepared using the μBs assisted FUS treatment, and edaravone had a therapeutic effect on this model. This model can be used to study the pathophysiological mechanism of ICH‐related diseases and in preclinical research on related new drugs.https://doi.org/10.1002/ame2.12303focused ultrasound (FUS)ICH modelmicrobubbles (μBs)rat |
| spellingShingle | Yao He Jie Yang Fengying Hu Min Liao Yuru Nie Xiaoxia Zhu Tao Zhang Keer Song Qinxi Li Xiaojie Li Chenghan Mei Zhe Wu Qiang Lu Zhihui Zhong A new method for preparing a rat intracerebral hemorrhage model by combining focused ultrasound and microbubbles Animal Models and Experimental Medicine focused ultrasound (FUS) ICH model microbubbles (μBs) rat |
| title | A new method for preparing a rat intracerebral hemorrhage model by combining focused ultrasound and microbubbles |
| title_full | A new method for preparing a rat intracerebral hemorrhage model by combining focused ultrasound and microbubbles |
| title_fullStr | A new method for preparing a rat intracerebral hemorrhage model by combining focused ultrasound and microbubbles |
| title_full_unstemmed | A new method for preparing a rat intracerebral hemorrhage model by combining focused ultrasound and microbubbles |
| title_short | A new method for preparing a rat intracerebral hemorrhage model by combining focused ultrasound and microbubbles |
| title_sort | new method for preparing a rat intracerebral hemorrhage model by combining focused ultrasound and microbubbles |
| topic | focused ultrasound (FUS) ICH model microbubbles (μBs) rat |
| url | https://doi.org/10.1002/ame2.12303 |
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