Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform
In this contribution a strategy is shown to covalently immobilize gold nanoparticles (AuNPs) onto vesicle bilayers with the aim of using this nanomaterial as platform for the future design of immunosensors. A novel methodology for the self-assembly of AuNPs onto large unilamellar vesicle structures...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Beilstein-Institut
2016-05-01
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| Series: | Beilstein Journal of Nanotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.3762/bjnano.7.58 |
| _version_ | 1818293844086620160 |
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| author | M. Fátima Barroso M. Alejandra Luna Juan S. Flores Tabares Cristina Delerue-Matos N. Mariano Correa Fernando Moyano Patricia G. Molina |
| author_facet | M. Fátima Barroso M. Alejandra Luna Juan S. Flores Tabares Cristina Delerue-Matos N. Mariano Correa Fernando Moyano Patricia G. Molina |
| author_sort | M. Fátima Barroso |
| collection | DOAJ |
| description | In this contribution a strategy is shown to covalently immobilize gold nanoparticles (AuNPs) onto vesicle bilayers with the aim of using this nanomaterial as platform for the future design of immunosensors. A novel methodology for the self-assembly of AuNPs onto large unilamellar vesicle structures is described. The vesicles were formed with 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-undecanethiol (SH). After, the AuNPs photochemically synthesized in pure glycerol were mixed and anchored onto SH–DOPC vesicles. The data provided by voltammetry, spectrometry and microscopy techniques indicated that the AuNPs were successfully covalently anchored onto the vesicle bilayer and decorated vesicles exhibit a spherical shape with a size of 190 ± 10 nm. The developed procedure is easy, rapid and reproducible to start designing a possible immunosensor by using environmentally friendly procedures. |
| first_indexed | 2024-12-13T03:22:18Z |
| format | Article |
| id | doaj.art-b0a97bf55bd54eefa40129a4f9c185c8 |
| institution | Directory Open Access Journal |
| issn | 2190-4286 |
| language | English |
| last_indexed | 2024-12-13T03:22:18Z |
| publishDate | 2016-05-01 |
| publisher | Beilstein-Institut |
| record_format | Article |
| series | Beilstein Journal of Nanotechnology |
| spelling | doaj.art-b0a97bf55bd54eefa40129a4f9c185c82022-12-22T00:01:20ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862016-05-017165566310.3762/bjnano.7.582190-4286-7-58Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platformM. Fátima Barroso0M. Alejandra Luna1Juan S. Flores Tabares2Cristina Delerue-Matos3N. Mariano Correa4Fernando Moyano5Patricia G. Molina6Departamento de Química. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Universidad Nacional de Río Cuarto. Agencia Postal Nº 3 - (5800) Río Cuarto, ArgentinaDepartamento de Química. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Universidad Nacional de Río Cuarto. Agencia Postal Nº 3 - (5800) Río Cuarto, ArgentinaDepartamento de Química. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Universidad Nacional de Río Cuarto. Agencia Postal Nº 3 - (5800) Río Cuarto, ArgentinaREQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida 431, 4200-072 Porto, PortugalDepartamento de Química. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Universidad Nacional de Río Cuarto. Agencia Postal Nº 3 - (5800) Río Cuarto, ArgentinaDepartamento de Química. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Universidad Nacional de Río Cuarto. Agencia Postal Nº 3 - (5800) Río Cuarto, ArgentinaDepartamento de Química. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Universidad Nacional de Río Cuarto. Agencia Postal Nº 3 - (5800) Río Cuarto, ArgentinaIn this contribution a strategy is shown to covalently immobilize gold nanoparticles (AuNPs) onto vesicle bilayers with the aim of using this nanomaterial as platform for the future design of immunosensors. A novel methodology for the self-assembly of AuNPs onto large unilamellar vesicle structures is described. The vesicles were formed with 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-undecanethiol (SH). After, the AuNPs photochemically synthesized in pure glycerol were mixed and anchored onto SH–DOPC vesicles. The data provided by voltammetry, spectrometry and microscopy techniques indicated that the AuNPs were successfully covalently anchored onto the vesicle bilayer and decorated vesicles exhibit a spherical shape with a size of 190 ± 10 nm. The developed procedure is easy, rapid and reproducible to start designing a possible immunosensor by using environmentally friendly procedures.https://doi.org/10.3762/bjnano.7.58covalently decorated vesiclesgold nanoparticlesimmunosensors designsynthesis |
| spellingShingle | M. Fátima Barroso M. Alejandra Luna Juan S. Flores Tabares Cristina Delerue-Matos N. Mariano Correa Fernando Moyano Patricia G. Molina Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform Beilstein Journal of Nanotechnology covalently decorated vesicles gold nanoparticles immunosensors design synthesis |
| title | Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform |
| title_full | Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform |
| title_fullStr | Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform |
| title_full_unstemmed | Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform |
| title_short | Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform |
| title_sort | gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform |
| topic | covalently decorated vesicles gold nanoparticles immunosensors design synthesis |
| url | https://doi.org/10.3762/bjnano.7.58 |
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