Cultivation of <i>Inonotus hispidus</i> in Stirred Tank and Wave Bag Bioreactors to Produce the Natural Colorant Hispidin
Hispidin (6-(3,4-dihydroxystyrl)-4-hydroxy-2-pyrone) production in submerged cultured mycelia of the basidiomycete <i>Inonotus hispidus</i> was doubled in shake flasks through irradiation with white light. The daily addition of 1 mM hydrogen peroxide as a chemical stressor and a repeated...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/10/541 |
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| author | Pia Bergmann Meike Takenberg Christina Frank Marlen Zschätzsch Anett Werner Ralf G. Berger Franziska Ersoy |
| author_facet | Pia Bergmann Meike Takenberg Christina Frank Marlen Zschätzsch Anett Werner Ralf G. Berger Franziska Ersoy |
| author_sort | Pia Bergmann |
| collection | DOAJ |
| description | Hispidin (6-(3,4-dihydroxystyrl)-4-hydroxy-2-pyrone) production in submerged cultured mycelia of the basidiomycete <i>Inonotus hispidus</i> was doubled in shake flasks through irradiation with white light. The daily addition of 1 mM hydrogen peroxide as a chemical stressor and a repeated supplementation of the shake flask cultures with 2 mM caffeic acid, a biogenetic precursor, further increased the hispidin synthesis. These cultivation conditions were combined and applied to parallel fermentation trials on the 4 L scale using a classical stirred tank bioreactor and a wave bag bioreactor. No significant differences in biomass yield and colorant production were observed. The hispidin concentration in both bioreactors reached 5.5 g·L<sup>−</sup><sup>1</sup>, the highest ever published. Textile dyeing with hispidin was successful, but impeded by its limited light stability in comparison to industrial dyes. However, following the idea of sustainability and the flawless toxicity profile, applications in natural cosmetics, other daily implements, or even therapeutics appear promising. |
| first_indexed | 2024-03-09T12:20:06Z |
| format | Article |
| id | doaj.art-b0aa3f53c1f64df8bf964b4eb06b83b9 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T12:20:06Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-b0aa3f53c1f64df8bf964b4eb06b83b92023-11-30T22:42:33ZengMDPI AGFermentation2311-56372022-10-0181054110.3390/fermentation8100541Cultivation of <i>Inonotus hispidus</i> in Stirred Tank and Wave Bag Bioreactors to Produce the Natural Colorant HispidinPia Bergmann0Meike Takenberg1Christina Frank2Marlen Zschätzsch3Anett Werner4Ralf G. Berger5Franziska Ersoy6Institute of Food Chemistry, Leibniz University Hannover, 30167 Hannover, GermanyInstitute of Food Chemistry, Leibniz University Hannover, 30167 Hannover, GermanyInstitute of Food Chemistry, Leibniz University Hannover, 30167 Hannover, GermanyInstitute of Natural Materials Technology, Technical University of Dresden, 01062 Dresden, GermanyInstitute of Natural Materials Technology, Technical University of Dresden, 01062 Dresden, GermanyInstitute of Food Chemistry, Leibniz University Hannover, 30167 Hannover, GermanyInstitute of Food Chemistry, Leibniz University Hannover, 30167 Hannover, GermanyHispidin (6-(3,4-dihydroxystyrl)-4-hydroxy-2-pyrone) production in submerged cultured mycelia of the basidiomycete <i>Inonotus hispidus</i> was doubled in shake flasks through irradiation with white light. The daily addition of 1 mM hydrogen peroxide as a chemical stressor and a repeated supplementation of the shake flask cultures with 2 mM caffeic acid, a biogenetic precursor, further increased the hispidin synthesis. These cultivation conditions were combined and applied to parallel fermentation trials on the 4 L scale using a classical stirred tank bioreactor and a wave bag bioreactor. No significant differences in biomass yield and colorant production were observed. The hispidin concentration in both bioreactors reached 5.5 g·L<sup>−</sup><sup>1</sup>, the highest ever published. Textile dyeing with hispidin was successful, but impeded by its limited light stability in comparison to industrial dyes. However, following the idea of sustainability and the flawless toxicity profile, applications in natural cosmetics, other daily implements, or even therapeutics appear promising.https://www.mdpi.com/2311-5637/8/10/541<i>Inonotus hispidus</i>hispidinbioprocessinductionelicitationprecursor |
| spellingShingle | Pia Bergmann Meike Takenberg Christina Frank Marlen Zschätzsch Anett Werner Ralf G. Berger Franziska Ersoy Cultivation of <i>Inonotus hispidus</i> in Stirred Tank and Wave Bag Bioreactors to Produce the Natural Colorant Hispidin Fermentation <i>Inonotus hispidus</i> hispidin bioprocess induction elicitation precursor |
| title | Cultivation of <i>Inonotus hispidus</i> in Stirred Tank and Wave Bag Bioreactors to Produce the Natural Colorant Hispidin |
| title_full | Cultivation of <i>Inonotus hispidus</i> in Stirred Tank and Wave Bag Bioreactors to Produce the Natural Colorant Hispidin |
| title_fullStr | Cultivation of <i>Inonotus hispidus</i> in Stirred Tank and Wave Bag Bioreactors to Produce the Natural Colorant Hispidin |
| title_full_unstemmed | Cultivation of <i>Inonotus hispidus</i> in Stirred Tank and Wave Bag Bioreactors to Produce the Natural Colorant Hispidin |
| title_short | Cultivation of <i>Inonotus hispidus</i> in Stirred Tank and Wave Bag Bioreactors to Produce the Natural Colorant Hispidin |
| title_sort | cultivation of i inonotus hispidus i in stirred tank and wave bag bioreactors to produce the natural colorant hispidin |
| topic | <i>Inonotus hispidus</i> hispidin bioprocess induction elicitation precursor |
| url | https://www.mdpi.com/2311-5637/8/10/541 |
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