Effects of cysteamine and vitamin E addition to semen extender on post-thawed microscopic sperm parameters of buffalo bulls
This study was designed to investigate the effect of cysteamine and vitamin E on post thaw buffalo bull's sperm quality. For this purpose, 20 ejaculates from four buffalo bulls possessing more than 70% visual sperm motility were diluted at 37◦C in BioXcell® extender. The diluted semen was coole...
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| Format: | Article |
| Language: | fas |
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Islamic Azad University, Tabriz Branch
2014-02-01
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| Series: | Āsīb/shināsī-i Darmāngāhī-i Dāmpizishkī |
| Subjects: | |
| Online Access: | http://jvcp.iaut.ac.ir/article_517218_8233f6a875b7fbebba58322f30ab0d85.pdf |
| _version_ | 1828752079913484288 |
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| author | rahim beheshti jamshid ghiyasi |
| author_facet | rahim beheshti jamshid ghiyasi |
| author_sort | rahim beheshti |
| collection | DOAJ |
| description | This study was designed to investigate the effect of cysteamine and vitamin E on post thaw buffalo bull's sperm quality. For this purpose, 20 ejaculates from four buffalo bulls possessing more than 70% visual sperm motility were diluted at 37◦C in BioXcell® extender. The diluted semen was cooled to 4◦C within 2 hours, equilibrated at 4◦C following the addition of (0.75, 1.5, 2 and 5 mM) of vitamin E and (7.5, 12.5, 15 and 20 mM) cysteamine per 90 ml, filled in 0.5 ml French straws and were subjected to cooling condition before being plunged into liquid nitrogen. Semen was thawed at 37◦C for 40 seconds after 72 hours of storage inside liquid nitrogen .Post-thaw sperm motility and some qualitative parameters of each frozen semen sample were assessed by using computer assisted semen analyzer (CASA). In general, the results showed that the addition of 1.5 mM vitamin E and 20 mM cysteamine in the commercial diluents BioXcell extender for freezing buffalo semen increased the motility of spermatozoa and some qualitative parameters to post-thawed buffalo sperm. |
| first_indexed | 2024-12-10T21:03:21Z |
| format | Article |
| id | doaj.art-b0aa7cb5d1bf478492b17d58c50df7f6 |
| institution | Directory Open Access Journal |
| issn | 2322-4746 2476-6984 |
| language | fas |
| last_indexed | 2024-12-10T21:03:21Z |
| publishDate | 2014-02-01 |
| publisher | Islamic Azad University, Tabriz Branch |
| record_format | Article |
| series | Āsīb/shināsī-i Darmāngāhī-i Dāmpizishkī |
| spelling | doaj.art-b0aa7cb5d1bf478492b17d58c50df7f62022-12-22T01:33:43ZfasIslamic Azad University, Tabriz BranchĀsīb/shināsī-i Darmāngāhī-i Dāmpizishkī2322-47462476-69842014-02-0174 (28) زمستان268276517218Effects of cysteamine and vitamin E addition to semen extender on post-thawed microscopic sperm parameters of buffalo bullsrahim beheshti0jamshid ghiyasi1دانشگاه آزاد اسلامی، واحد شبستر، استادیار گروه علوم درمانگاهی، شبستر، ایران.دانشگاه آزاد اسلامی، واحد شبستر، استادیار گروه علوم پایه، شبستر، ایران.This study was designed to investigate the effect of cysteamine and vitamin E on post thaw buffalo bull's sperm quality. For this purpose, 20 ejaculates from four buffalo bulls possessing more than 70% visual sperm motility were diluted at 37◦C in BioXcell® extender. The diluted semen was cooled to 4◦C within 2 hours, equilibrated at 4◦C following the addition of (0.75, 1.5, 2 and 5 mM) of vitamin E and (7.5, 12.5, 15 and 20 mM) cysteamine per 90 ml, filled in 0.5 ml French straws and were subjected to cooling condition before being plunged into liquid nitrogen. Semen was thawed at 37◦C for 40 seconds after 72 hours of storage inside liquid nitrogen .Post-thaw sperm motility and some qualitative parameters of each frozen semen sample were assessed by using computer assisted semen analyzer (CASA). In general, the results showed that the addition of 1.5 mM vitamin E and 20 mM cysteamine in the commercial diluents BioXcell extender for freezing buffalo semen increased the motility of spermatozoa and some qualitative parameters to post-thawed buffalo sperm.http://jvcp.iaut.ac.ir/article_517218_8233f6a875b7fbebba58322f30ab0d85.pdfSemen freezingBuffalovitamin ECysteamine |
| spellingShingle | rahim beheshti jamshid ghiyasi Effects of cysteamine and vitamin E addition to semen extender on post-thawed microscopic sperm parameters of buffalo bulls Āsīb/shināsī-i Darmāngāhī-i Dāmpizishkī Semen freezing Buffalo vitamin E Cysteamine |
| title | Effects of cysteamine and vitamin E addition to semen extender on
post-thawed microscopic sperm parameters of buffalo bulls |
| title_full | Effects of cysteamine and vitamin E addition to semen extender on
post-thawed microscopic sperm parameters of buffalo bulls |
| title_fullStr | Effects of cysteamine and vitamin E addition to semen extender on
post-thawed microscopic sperm parameters of buffalo bulls |
| title_full_unstemmed | Effects of cysteamine and vitamin E addition to semen extender on
post-thawed microscopic sperm parameters of buffalo bulls |
| title_short | Effects of cysteamine and vitamin E addition to semen extender on
post-thawed microscopic sperm parameters of buffalo bulls |
| title_sort | effects of cysteamine and vitamin e addition to semen extender on post thawed microscopic sperm parameters of buffalo bulls |
| topic | Semen freezing Buffalo vitamin E Cysteamine |
| url | http://jvcp.iaut.ac.ir/article_517218_8233f6a875b7fbebba58322f30ab0d85.pdf |
| work_keys_str_mv | AT rahimbeheshti effectsofcysteamineandvitamineadditiontosemenextenderonpostthawedmicroscopicspermparametersofbuffalobulls AT jamshidghiyasi effectsofcysteamineandvitamineadditiontosemenextenderonpostthawedmicroscopicspermparametersofbuffalobulls |