Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections
Abstract Animal African trypanosomosis (AAT), a disease affecting livestock, is caused by parasites of the Trypanosoma genus (mainly T. vivax and T. congolense). AAT is widespread in Sub-Saharan Africa, where it continues to impose a heavy socio-economic burden as it renders development of sustainab...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2018-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-018-26732-7 |
| _version_ | 1818994378280009728 |
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| author | Joar E. Pinto Torres Julie Goossens Jianzu Ding Zeng Li Shaohong Lu Didier Vertommen Peter Naniima Rui Chen Serge Muyldermans Yann G.-J. Sterckx Stefan Magez |
| author_facet | Joar E. Pinto Torres Julie Goossens Jianzu Ding Zeng Li Shaohong Lu Didier Vertommen Peter Naniima Rui Chen Serge Muyldermans Yann G.-J. Sterckx Stefan Magez |
| author_sort | Joar E. Pinto Torres |
| collection | DOAJ |
| description | Abstract Animal African trypanosomosis (AAT), a disease affecting livestock, is caused by parasites of the Trypanosoma genus (mainly T. vivax and T. congolense). AAT is widespread in Sub-Saharan Africa, where it continues to impose a heavy socio-economic burden as it renders development of sustainable livestock rearing very strenuous. Active case-finding and the identification of infected animals prior to initiation of drug treatment requires the availability of sensitive and specific diagnostic tests. In this paper, we describe the development of two heterologous sandwich assay formats (ELISA and LFA) for T. congolense detection through the use of Nanobodies (Nbs). The immunisation of an alpaca with a secretome mix from two T. congolense strains resulted in the identification of a Nb pair (Nb44/Nb42) that specifically targets the glycolytic enzyme pyruvate kinase. We demonstrate that the Nb44/Nb42 ELISA and LFA can be employed to detect parasitaemia in plasma samples from experimentally infected mice and cattle and, additionally, that they can serve as ‘test-of-cure’ tools. Altogether, the findings in this paper present the development and evaluation of the first Nb-based antigen detection LFA to identify active T. congolense infections. |
| first_indexed | 2024-12-20T20:57:00Z |
| format | Article |
| id | doaj.art-b0ab489491d949b589362f495b917920 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T20:57:00Z |
| publishDate | 2018-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b0ab489491d949b589362f495b9179202022-12-21T19:26:49ZengNature PortfolioScientific Reports2045-23222018-06-018111510.1038/s41598-018-26732-7Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infectionsJoar E. Pinto Torres0Julie Goossens1Jianzu Ding2Zeng Li3Shaohong Lu4Didier Vertommen5Peter Naniima6Rui Chen7Serge Muyldermans8Yann G.-J. Sterckx9Stefan Magez10Research Unit for Cellular and Molecular Immunology (CMIM)Research Unit for Cellular and Molecular Immunology (CMIM)Institute of Parasitic Diseases, Zhejiang Academy of Medical SciencesResearch Unit for Cellular and Molecular Immunology (CMIM)Institute of Parasitic Diseases, Zhejiang Academy of Medical SciencesDepartment of Metabolism and Hormones, de Duve Institute, Université Catholique de LouvainInstitute of Virology, Structural Virology Group, Medizinische Hochschule HannoverInstitute of Parasitic Diseases, Zhejiang Academy of Medical SciencesResearch Unit for Cellular and Molecular Immunology (CMIM)Research Unit for Cellular and Molecular Immunology (CMIM)Research Unit for Cellular and Molecular Immunology (CMIM)Abstract Animal African trypanosomosis (AAT), a disease affecting livestock, is caused by parasites of the Trypanosoma genus (mainly T. vivax and T. congolense). AAT is widespread in Sub-Saharan Africa, where it continues to impose a heavy socio-economic burden as it renders development of sustainable livestock rearing very strenuous. Active case-finding and the identification of infected animals prior to initiation of drug treatment requires the availability of sensitive and specific diagnostic tests. In this paper, we describe the development of two heterologous sandwich assay formats (ELISA and LFA) for T. congolense detection through the use of Nanobodies (Nbs). The immunisation of an alpaca with a secretome mix from two T. congolense strains resulted in the identification of a Nb pair (Nb44/Nb42) that specifically targets the glycolytic enzyme pyruvate kinase. We demonstrate that the Nb44/Nb42 ELISA and LFA can be employed to detect parasitaemia in plasma samples from experimentally infected mice and cattle and, additionally, that they can serve as ‘test-of-cure’ tools. Altogether, the findings in this paper present the development and evaluation of the first Nb-based antigen detection LFA to identify active T. congolense infections.https://doi.org/10.1038/s41598-018-26732-7 |
| spellingShingle | Joar E. Pinto Torres Julie Goossens Jianzu Ding Zeng Li Shaohong Lu Didier Vertommen Peter Naniima Rui Chen Serge Muyldermans Yann G.-J. Sterckx Stefan Magez Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections Scientific Reports |
| title | Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections |
| title_full | Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections |
| title_fullStr | Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections |
| title_full_unstemmed | Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections |
| title_short | Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections |
| title_sort | development of a nanobody based lateral flow assay to detect active trypanosoma congolense infections |
| url | https://doi.org/10.1038/s41598-018-26732-7 |
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