Reprogramming of a human induced pluripotent stem cell line from one 48-year-old healthy male donor
In this study, skin biopsy was collected from a healthy 48-year old male donor with informed consent, and the fibroblasts were isolated from the dermal explant cultures. Here, a human induced pluripotent stem cell (iPSC) line was derived from the fibroblasts using the reprogramming four Yamanaka fac...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-05-01
|
| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506121001859 |
| _version_ | 1818730213415059456 |
|---|---|
| author | Ruiyun Guo Weihong Mu Xin Liu Jing Zhang Boxin Liu Xiaofeng Du Jingjing He Jun Ma Huixian Cui |
| author_facet | Ruiyun Guo Weihong Mu Xin Liu Jing Zhang Boxin Liu Xiaofeng Du Jingjing He Jun Ma Huixian Cui |
| author_sort | Ruiyun Guo |
| collection | DOAJ |
| description | In this study, skin biopsy was collected from a healthy 48-year old male donor with informed consent, and the fibroblasts were isolated from the dermal explant cultures. Here, a human induced pluripotent stem cell (iPSC) line was derived from the fibroblasts using the reprogramming four Yamanaka factors (Oct3/4, Sox2, Klf4, c-Myc). The generated iPSCs were integration-free, displayed the normal karyotype, expressed pluripotency markers and demonstrated trilineage differentiation potential in vitro. This iPSC model will be useful for investigating physiological processes, drug validation as well as a control in pathological mechanistic studies. |
| first_indexed | 2024-12-17T22:58:12Z |
| format | Article |
| id | doaj.art-b0adcd6b1f81408a9deb0dfb99029afd |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-17T22:58:12Z |
| publishDate | 2021-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-b0adcd6b1f81408a9deb0dfb99029afd2022-12-21T21:29:29ZengElsevierStem Cell Research1873-50612021-05-0153102339Reprogramming of a human induced pluripotent stem cell line from one 48-year-old healthy male donorRuiyun Guo0Weihong Mu1Xin Liu2Jing Zhang3Boxin Liu4Xiaofeng Du5Jingjing He6Jun Ma7Huixian Cui8Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, ChinaCenter of Prenatal Diagnosis, Shijiazhuang Obstetrics and Gynecology Hospital, Shijiazhuang, Hebei 050011, ChinaHebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, ChinaCenter of Prenatal Diagnosis, Shijiazhuang Obstetrics and Gynecology Hospital, Shijiazhuang, Hebei 050011, ChinaHebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, ChinaHebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, ChinaHebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, ChinaHebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Human Anatomy Department, Hebei Medical University, Hebei Province 050017, China; Corresponding authors at: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China.Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Human Anatomy Department, Hebei Medical University, Hebei Province 050017, China; Corresponding authors at: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China.In this study, skin biopsy was collected from a healthy 48-year old male donor with informed consent, and the fibroblasts were isolated from the dermal explant cultures. Here, a human induced pluripotent stem cell (iPSC) line was derived from the fibroblasts using the reprogramming four Yamanaka factors (Oct3/4, Sox2, Klf4, c-Myc). The generated iPSCs were integration-free, displayed the normal karyotype, expressed pluripotency markers and demonstrated trilineage differentiation potential in vitro. This iPSC model will be useful for investigating physiological processes, drug validation as well as a control in pathological mechanistic studies.http://www.sciencedirect.com/science/article/pii/S1873506121001859 |
| spellingShingle | Ruiyun Guo Weihong Mu Xin Liu Jing Zhang Boxin Liu Xiaofeng Du Jingjing He Jun Ma Huixian Cui Reprogramming of a human induced pluripotent stem cell line from one 48-year-old healthy male donor Stem Cell Research |
| title | Reprogramming of a human induced pluripotent stem cell line from one 48-year-old healthy male donor |
| title_full | Reprogramming of a human induced pluripotent stem cell line from one 48-year-old healthy male donor |
| title_fullStr | Reprogramming of a human induced pluripotent stem cell line from one 48-year-old healthy male donor |
| title_full_unstemmed | Reprogramming of a human induced pluripotent stem cell line from one 48-year-old healthy male donor |
| title_short | Reprogramming of a human induced pluripotent stem cell line from one 48-year-old healthy male donor |
| title_sort | reprogramming of a human induced pluripotent stem cell line from one 48 year old healthy male donor |
| url | http://www.sciencedirect.com/science/article/pii/S1873506121001859 |
| work_keys_str_mv | AT ruiyunguo reprogrammingofahumaninducedpluripotentstemcelllinefromone48yearoldhealthymaledonor AT weihongmu reprogrammingofahumaninducedpluripotentstemcelllinefromone48yearoldhealthymaledonor AT xinliu reprogrammingofahumaninducedpluripotentstemcelllinefromone48yearoldhealthymaledonor AT jingzhang reprogrammingofahumaninducedpluripotentstemcelllinefromone48yearoldhealthymaledonor AT boxinliu reprogrammingofahumaninducedpluripotentstemcelllinefromone48yearoldhealthymaledonor AT xiaofengdu reprogrammingofahumaninducedpluripotentstemcelllinefromone48yearoldhealthymaledonor AT jingjinghe reprogrammingofahumaninducedpluripotentstemcelllinefromone48yearoldhealthymaledonor AT junma reprogrammingofahumaninducedpluripotentstemcelllinefromone48yearoldhealthymaledonor AT huixiancui reprogrammingofahumaninducedpluripotentstemcelllinefromone48yearoldhealthymaledonor |