Association of pvl gene with incomplete hemolytic phenotype in clinical Staphylococcus aureus
Menglu Gao,1 Ruirui Sang,1 Gang Wang,1 Yuanhong Xu1,21Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, 230000, People’s Republic of China; 2Provincial Laboratories of Pathogen Biology and Zoonoses, Anhui Medical University, Anhui, Hefei 2...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2019-06-01
|
| Series: | Infection and Drug Resistance |
| Subjects: | |
| Online Access: | https://www.dovepress.com/association-of-pvl-gene-with-incomplete-hemolytic-phenotype-in-clinica-peer-reviewed-article-IDR |
| _version_ | 1819227577863110656 |
|---|---|
| author | Gao M Sang R Wang G Xu Y |
| author_facet | Gao M Sang R Wang G Xu Y |
| author_sort | Gao M |
| collection | DOAJ |
| description | Menglu Gao,1 Ruirui Sang,1 Gang Wang,1 Yuanhong Xu1,21Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, 230000, People’s Republic of China; 2Provincial Laboratories of Pathogen Biology and Zoonoses, Anhui Medical University, Anhui, Hefei 230000, People’s Republic of ChinaPurpose: This experiment aimed to evaluate the correlation between the hemolytic phenotype of Staphylococcus aureus and pvl gene in terms of characteristics of antibiotic resistance.Materials and methods: Two-hundred and eleven strains of hospital-acquired S. aureus and their bacterial susceptibility to 20 antibiotics were determined by MicroScan WalkAway96. All strains were cultured on Columbia sheep blood agar plates for 24 hours and then underwent ten passages for investigation of their hemolytic phenotypes. S. aureus produced incomplete β-hemolytic phenotype, termed as S. aureus strains with incomplete hemolytic phenotype (SIHP). The pvl gene was identified by PCR amplification followed by DNA sequencing. Statistical analyses of the data were performed using SPSS version 16.0 software.Results: Fifty-two (24.64%) strains were confirmed to maintain the incomplete hemolytic phenotype of S. aureus (SIHP). Meanwhile, 15 (7.11%) of 211 strains were found to carry the pvl gene, and eight of the 15 strains were SIHP. Compared with S. aureus strains with complete hemolytic phenotype (SCHP), SIHP showed higher susceptibility to seven of the 20 antibiotics (oxacillin, ciprofloxacin, gentamicin, ceftriaxone, cefoxitin, levofloxacin, and moxifloxacin) (P<0.05). The pvl-positive bacteria had a higher rate of resistance to four antibiotics (rifampin, ciprofloxacin, levofloxacin, and moxifloxacin) in comparison with the pvl-negative strains (P<0.05).Conclusion: SIHP had a high frequency of pvl gene. The pvl-positive isolates showed less resistance to rifampin, ciprofloxacin, levofloxacin, and moxifloxacin. Additionally, the majority of SIHP isolates (61.54%) were methicillin-resistant S. aureus. SIHP strains had significantly higher antibiotic resistance to cefoxitin when compared with SCHP, while SCHP strains had a high rate of antibiotic resistance to ciprofloxacin, gentamicin, ceftriaxone, levofloxacin, and moxifloxacin. The results may help to provide medical advice for selection of antibiotics for patients with SIHP-associated infections.Keywords: S. aureus, incomplete hemolytic phenotype, pvl, antimicrobial drug resistance, virulence factor |
| first_indexed | 2024-12-23T10:43:36Z |
| format | Article |
| id | doaj.art-b0b0be7b0a7c4e98bfacb61c56bad259 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-23T10:43:36Z |
| publishDate | 2019-06-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-b0b0be7b0a7c4e98bfacb61c56bad2592022-12-21T17:50:06ZengDove Medical PressInfection and Drug Resistance1178-69732019-06-01Volume 121649165646459Association of pvl gene with incomplete hemolytic phenotype in clinical Staphylococcus aureusGao MSang RWang GXu YMenglu Gao,1 Ruirui Sang,1 Gang Wang,1 Yuanhong Xu1,21Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, 230000, People’s Republic of China; 2Provincial Laboratories of Pathogen Biology and Zoonoses, Anhui Medical University, Anhui, Hefei 230000, People’s Republic of ChinaPurpose: This experiment aimed to evaluate the correlation between the hemolytic phenotype of Staphylococcus aureus and pvl gene in terms of characteristics of antibiotic resistance.Materials and methods: Two-hundred and eleven strains of hospital-acquired S. aureus and their bacterial susceptibility to 20 antibiotics were determined by MicroScan WalkAway96. All strains were cultured on Columbia sheep blood agar plates for 24 hours and then underwent ten passages for investigation of their hemolytic phenotypes. S. aureus produced incomplete β-hemolytic phenotype, termed as S. aureus strains with incomplete hemolytic phenotype (SIHP). The pvl gene was identified by PCR amplification followed by DNA sequencing. Statistical analyses of the data were performed using SPSS version 16.0 software.Results: Fifty-two (24.64%) strains were confirmed to maintain the incomplete hemolytic phenotype of S. aureus (SIHP). Meanwhile, 15 (7.11%) of 211 strains were found to carry the pvl gene, and eight of the 15 strains were SIHP. Compared with S. aureus strains with complete hemolytic phenotype (SCHP), SIHP showed higher susceptibility to seven of the 20 antibiotics (oxacillin, ciprofloxacin, gentamicin, ceftriaxone, cefoxitin, levofloxacin, and moxifloxacin) (P<0.05). The pvl-positive bacteria had a higher rate of resistance to four antibiotics (rifampin, ciprofloxacin, levofloxacin, and moxifloxacin) in comparison with the pvl-negative strains (P<0.05).Conclusion: SIHP had a high frequency of pvl gene. The pvl-positive isolates showed less resistance to rifampin, ciprofloxacin, levofloxacin, and moxifloxacin. Additionally, the majority of SIHP isolates (61.54%) were methicillin-resistant S. aureus. SIHP strains had significantly higher antibiotic resistance to cefoxitin when compared with SCHP, while SCHP strains had a high rate of antibiotic resistance to ciprofloxacin, gentamicin, ceftriaxone, levofloxacin, and moxifloxacin. The results may help to provide medical advice for selection of antibiotics for patients with SIHP-associated infections.Keywords: S. aureus, incomplete hemolytic phenotype, pvl, antimicrobial drug resistance, virulence factorhttps://www.dovepress.com/association-of-pvl-gene-with-incomplete-hemolytic-phenotype-in-clinica-peer-reviewed-article-IDRS. aureusIncomplete hemolytic phenotypePVLAntimicrobial drug resistanceVirulence factor |
| spellingShingle | Gao M Sang R Wang G Xu Y Association of pvl gene with incomplete hemolytic phenotype in clinical Staphylococcus aureus Infection and Drug Resistance S. aureus Incomplete hemolytic phenotype PVL Antimicrobial drug resistance Virulence factor |
| title | Association of pvl gene with incomplete hemolytic phenotype in clinical Staphylococcus aureus |
| title_full | Association of pvl gene with incomplete hemolytic phenotype in clinical Staphylococcus aureus |
| title_fullStr | Association of pvl gene with incomplete hemolytic phenotype in clinical Staphylococcus aureus |
| title_full_unstemmed | Association of pvl gene with incomplete hemolytic phenotype in clinical Staphylococcus aureus |
| title_short | Association of pvl gene with incomplete hemolytic phenotype in clinical Staphylococcus aureus |
| title_sort | association of pvl gene with incomplete hemolytic phenotype in clinical staphylococcus aureus |
| topic | S. aureus Incomplete hemolytic phenotype PVL Antimicrobial drug resistance Virulence factor |
| url | https://www.dovepress.com/association-of-pvl-gene-with-incomplete-hemolytic-phenotype-in-clinica-peer-reviewed-article-IDR |
| work_keys_str_mv | AT gaom associationofpvlgenewithincompletehemolyticphenotypeinclinicalstaphylococcusaureus AT sangr associationofpvlgenewithincompletehemolyticphenotypeinclinicalstaphylococcusaureus AT wangg associationofpvlgenewithincompletehemolyticphenotypeinclinicalstaphylococcusaureus AT xuy associationofpvlgenewithincompletehemolyticphenotypeinclinicalstaphylococcusaureus |