The Effects of <i>Cannabis sativa</i> L. Extract on Oxidative Stress Markers In Vivo
In recent decades, a lot of attention has been paid to <i>Cannabis sativa</i> L. due to its useful applications, including in fibers, oil, food for humans and animals, and therapeutics. The present study aimed to determine antioxidant activity of cannabinoids in <i>Cannabis sativa&...
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MDPI AG
2021-07-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/11/7/647 |
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| author | Asta Kubiliene Karolina Mickute Juste Baranauskaite Mindaugas Marksa Arunas Liekis Ilona Sadauskiene |
| author_facet | Asta Kubiliene Karolina Mickute Juste Baranauskaite Mindaugas Marksa Arunas Liekis Ilona Sadauskiene |
| author_sort | Asta Kubiliene |
| collection | DOAJ |
| description | In recent decades, a lot of attention has been paid to <i>Cannabis sativa</i> L. due to its useful applications, including in fibers, oil, food for humans and animals, and therapeutics. The present study aimed to determine antioxidant activity of cannabinoids in <i>Cannabis sativa</i> L. in vivo, evaluating the possible antioxidative effect of <i>Cannabis sativa</i> L. extract (CE) on malondialdehyde (MDA) and glutathione (GSH) concentrations as well as on catalase (CAT) activity in BALB/c mice. In total, 40 mice were divided into five equal groups: the aluminum group (7.5 mg AlCl<sub>3</sub>/kg/d (0.15 LD<sub>50</sub>), the saline group, the 10% ethanol group (an appropriate amount of the solution for mouse weight), the CE group (1.6 mg CE/g/day), and the aluminum-CE group (7.5 mg AlCl<sub>3</sub> plus 1.6 mg CE/g/day). The results of the study showed that CE significantly decreased (by 26.81%, <i>p</i> < 0.05) the concentration of GSH in blood of the mice and the concentration of MDA in the brain (by 82.12%) and liver (by 53.5%) of the mice compared to the respective concentrations in the AlCl<sub>3</sub> group. CE significantly (<i>p</i> < 0.05) increased CAT activity in the brain (by 64.79%) and liver (by 72.37%) of the mice after the AlCl<sub>3</sub>-induced prooxidant effect. The results showed the antioxidant activity of cannabidiolic acid (CBDA) in vitro. The findings in vivo indicate that <i>Cannabis sativa</i> L. is a good source of natural antioxidants and can be used in the management of oxidative stress. |
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| format | Article |
| id | doaj.art-b0b36a28440d418995aae188d892afbe |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T09:34:35Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-b0b36a28440d418995aae188d892afbe2023-11-22T04:12:31ZengMDPI AGLife2075-17292021-07-0111764710.3390/life11070647The Effects of <i>Cannabis sativa</i> L. Extract on Oxidative Stress Markers In VivoAsta Kubiliene0Karolina Mickute1Juste Baranauskaite2Mindaugas Marksa3Arunas Liekis4Ilona Sadauskiene5Department of Analytical and Toxicological Chemistry, Faculty of Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Sukileliu St. 13, LT-50161 Kaunas, LithuaniaDepartment of Analytical and Toxicological Chemistry, Faculty of Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Sukileliu St. 13, LT-50161 Kaunas, LithuaniaDepartment of Analytical and Toxicological Chemistry, Faculty of Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Sukileliu St. 13, LT-50161 Kaunas, LithuaniaDepartment of Analytical and Toxicological Chemistry, Faculty of Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Sukileliu St. 13, LT-50161 Kaunas, LithuaniaNeuroscience Institute, Lithuanian University of Health Sciences, Eiveniu St. 4, LT-50161 Kaunas, LithuaniaNeuroscience Institute, Lithuanian University of Health Sciences, Eiveniu St. 4, LT-50161 Kaunas, LithuaniaIn recent decades, a lot of attention has been paid to <i>Cannabis sativa</i> L. due to its useful applications, including in fibers, oil, food for humans and animals, and therapeutics. The present study aimed to determine antioxidant activity of cannabinoids in <i>Cannabis sativa</i> L. in vivo, evaluating the possible antioxidative effect of <i>Cannabis sativa</i> L. extract (CE) on malondialdehyde (MDA) and glutathione (GSH) concentrations as well as on catalase (CAT) activity in BALB/c mice. In total, 40 mice were divided into five equal groups: the aluminum group (7.5 mg AlCl<sub>3</sub>/kg/d (0.15 LD<sub>50</sub>), the saline group, the 10% ethanol group (an appropriate amount of the solution for mouse weight), the CE group (1.6 mg CE/g/day), and the aluminum-CE group (7.5 mg AlCl<sub>3</sub> plus 1.6 mg CE/g/day). The results of the study showed that CE significantly decreased (by 26.81%, <i>p</i> < 0.05) the concentration of GSH in blood of the mice and the concentration of MDA in the brain (by 82.12%) and liver (by 53.5%) of the mice compared to the respective concentrations in the AlCl<sub>3</sub> group. CE significantly (<i>p</i> < 0.05) increased CAT activity in the brain (by 64.79%) and liver (by 72.37%) of the mice after the AlCl<sub>3</sub>-induced prooxidant effect. The results showed the antioxidant activity of cannabidiolic acid (CBDA) in vitro. The findings in vivo indicate that <i>Cannabis sativa</i> L. is a good source of natural antioxidants and can be used in the management of oxidative stress.https://www.mdpi.com/2075-1729/11/7/647<i>Cannabis</i>HPLCantioxidant activitymice |
| spellingShingle | Asta Kubiliene Karolina Mickute Juste Baranauskaite Mindaugas Marksa Arunas Liekis Ilona Sadauskiene The Effects of <i>Cannabis sativa</i> L. Extract on Oxidative Stress Markers In Vivo Life <i>Cannabis</i> HPLC antioxidant activity mice |
| title | The Effects of <i>Cannabis sativa</i> L. Extract on Oxidative Stress Markers In Vivo |
| title_full | The Effects of <i>Cannabis sativa</i> L. Extract on Oxidative Stress Markers In Vivo |
| title_fullStr | The Effects of <i>Cannabis sativa</i> L. Extract on Oxidative Stress Markers In Vivo |
| title_full_unstemmed | The Effects of <i>Cannabis sativa</i> L. Extract on Oxidative Stress Markers In Vivo |
| title_short | The Effects of <i>Cannabis sativa</i> L. Extract on Oxidative Stress Markers In Vivo |
| title_sort | effects of i cannabis sativa i l extract on oxidative stress markers in vivo |
| topic | <i>Cannabis</i> HPLC antioxidant activity mice |
| url | https://www.mdpi.com/2075-1729/11/7/647 |
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