Reversibility and safety of KISS1 metastasis suppressor gene vaccine in immunocastration of ram lambs
Objective The aim of this study was to investigate the reversibility and safety of KISS1 metastasis suppressor (KISS1) gene vaccine in immunocastration. Methods Six eight-week old ram lambs were randomly divided into vaccinated and control groups. The vaccine (1 mg/ram lamb) was injected at weeks 0,...
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Asian-Australasian Association of Animal Production Societies
2018-06-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-31-6-835.pdf |
| _version_ | 1818585049204785152 |
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| author | Yan-Guo Han Gui-Qiong Liu Xun-Ping Jiang Xing-Long Xiang Yong-Fu Huang Bin Nie Jia-Yu Zhao Ijaz Nabeel Birhanu Tesema |
| author_facet | Yan-Guo Han Gui-Qiong Liu Xun-Ping Jiang Xing-Long Xiang Yong-Fu Huang Bin Nie Jia-Yu Zhao Ijaz Nabeel Birhanu Tesema |
| author_sort | Yan-Guo Han |
| collection | DOAJ |
| description | Objective The aim of this study was to investigate the reversibility and safety of KISS1 metastasis suppressor (KISS1) gene vaccine in immunocastration. Methods Six eight-week old ram lambs were randomly divided into vaccinated and control groups. The vaccine (1 mg/ram lamb) was injected at weeks 0, 3, and 6 of the study. Blood samples were collected from the jugular vein before primary immunization and at weeks 2, 4, 6, 10, 14, 22, and 30 after primary immunization. All ram lambs were slaughtered at 38 weeks of age, and samples were collected. Results The specific anti-KISS1 antibody titers in vaccinated animals were significantly higher and the serum testosterone level was significantly lower than those in the control groups from week 4 to 14 after primary immunization (p<0.05). No significant difference was observed at weeks 22 and 30 after the primary immunization. Similar results were also found for scrotal circumference, testicular weight, length, breadth, and spermatogenesis in seminiferous tubules in week 30 after primary immunization. KS (KISS1-hepatitis B surface antigen S) fusion fragment of KISS1 gene vaccine was not detected in host cell genomic DNA of 9 tissues of the vaccinated ram lambs by polymerase chain reaction. Conclusion The effects of KISS1 gene vaccine in immunocastration were reversible and no integration events were recorded. |
| first_indexed | 2024-12-16T08:30:53Z |
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| id | doaj.art-b0b39ad66be2434a8fd52ab6c00d02fd |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-16T08:30:53Z |
| publishDate | 2018-06-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-b0b39ad66be2434a8fd52ab6c00d02fd2022-12-21T22:37:52ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172018-06-0131683584110.5713/ajas.17.062923901Reversibility and safety of KISS1 metastasis suppressor gene vaccine in immunocastration of ram lambsYan-Guo Han0Gui-Qiong Liu1Xun-Ping Jiang2Xing-Long Xiang3Yong-Fu Huang4Bin Nie5Jia-Yu Zhao6Ijaz Nabeel7Birhanu Tesema8 Chongqing Key Laboratory of Forage & Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, College of Animal Science and Technology, Southwest University, Chongqing 400715, China Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China Chongqing Key Laboratory of Forage & Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, College of Animal Science and Technology, Southwest University, Chongqing 400715, China Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaObjective The aim of this study was to investigate the reversibility and safety of KISS1 metastasis suppressor (KISS1) gene vaccine in immunocastration. Methods Six eight-week old ram lambs were randomly divided into vaccinated and control groups. The vaccine (1 mg/ram lamb) was injected at weeks 0, 3, and 6 of the study. Blood samples were collected from the jugular vein before primary immunization and at weeks 2, 4, 6, 10, 14, 22, and 30 after primary immunization. All ram lambs were slaughtered at 38 weeks of age, and samples were collected. Results The specific anti-KISS1 antibody titers in vaccinated animals were significantly higher and the serum testosterone level was significantly lower than those in the control groups from week 4 to 14 after primary immunization (p<0.05). No significant difference was observed at weeks 22 and 30 after the primary immunization. Similar results were also found for scrotal circumference, testicular weight, length, breadth, and spermatogenesis in seminiferous tubules in week 30 after primary immunization. KS (KISS1-hepatitis B surface antigen S) fusion fragment of KISS1 gene vaccine was not detected in host cell genomic DNA of 9 tissues of the vaccinated ram lambs by polymerase chain reaction. Conclusion The effects of KISS1 gene vaccine in immunocastration were reversible and no integration events were recorded.http://www.ajas.info/upload/pdf/ajas-31-6-835.pdfKISS1 Metastasis SuppressorGene VaccineImmunocastrationReversibilitySafetyRam Lambs |
| spellingShingle | Yan-Guo Han Gui-Qiong Liu Xun-Ping Jiang Xing-Long Xiang Yong-Fu Huang Bin Nie Jia-Yu Zhao Ijaz Nabeel Birhanu Tesema Reversibility and safety of KISS1 metastasis suppressor gene vaccine in immunocastration of ram lambs Asian-Australasian Journal of Animal Sciences KISS1 Metastasis Suppressor Gene Vaccine Immunocastration Reversibility Safety Ram Lambs |
| title | Reversibility and safety of KISS1 metastasis suppressor gene vaccine in immunocastration of ram lambs |
| title_full | Reversibility and safety of KISS1 metastasis suppressor gene vaccine in immunocastration of ram lambs |
| title_fullStr | Reversibility and safety of KISS1 metastasis suppressor gene vaccine in immunocastration of ram lambs |
| title_full_unstemmed | Reversibility and safety of KISS1 metastasis suppressor gene vaccine in immunocastration of ram lambs |
| title_short | Reversibility and safety of KISS1 metastasis suppressor gene vaccine in immunocastration of ram lambs |
| title_sort | reversibility and safety of kiss1 metastasis suppressor gene vaccine in immunocastration of ram lambs |
| topic | KISS1 Metastasis Suppressor Gene Vaccine Immunocastration Reversibility Safety Ram Lambs |
| url | http://www.ajas.info/upload/pdf/ajas-31-6-835.pdf |
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