Detection and biological control measures of anthracnose causing fungus isolated from Citrus limon (L.)
Anthracnose is one of the most devastating fungi causing twig dieback and postharvest fruit decay. Present study was aimed to identification of anthracnose fungus. Infected leaves of Citrus limon were collected and cultured on potato dextrose agar (PDA) media for pathogenic fungus isolation. The iso...
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| Format: | Article |
| Language: | English |
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Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
2023-04-01
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| Series: | Journal of Advanced Biotechnology and Experimental Therapeutics |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=68559 |
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| author | Md Faruk Hasan Jannatul Ferdous Amit Kumar Dutta Fahmida Begum Mina Sumon Karmakar Biswanath Sikdar |
| author_facet | Md Faruk Hasan Jannatul Ferdous Amit Kumar Dutta Fahmida Begum Mina Sumon Karmakar Biswanath Sikdar |
| author_sort | Md Faruk Hasan |
| collection | DOAJ |
| description | Anthracnose is one of the most devastating fungi causing twig dieback and postharvest fruit decay. Present study was aimed to identification of anthracnose fungus. Infected leaves of Citrus limon were collected and cultured on potato dextrose agar (PDA) media for pathogenic fungus isolation. The isolated fungal pure culture was characterized by physiological and morphological characterization methods. Biological control measures of the fungus were evaluated by disc diffusion methods. The highest growth and development of isolated fungus was detected in PDA media pH 7 in fructose as the best carbon source and 0.05gm NaCl concentrations at 37°C. Pathogenicity potency of isolate was performed on lemon, orange and malta, belonging to the Citrus sp. For antifungal activity, 200µgm/disc methanolic extract of Psidium guajava showed 14.33±0.66 mm inhibition zone against the isolated fungus. From the present investigations, identified anthracnose causing fungus and it's controlling techniques may help for further research for the isolation of drugs related compound for controlling this disease. [ J Adv Biotechnol Exp Ther 2023; 6(1.000): 58-66] |
| first_indexed | 2024-04-11T04:11:01Z |
| format | Article |
| id | doaj.art-b0b4402072ca47e08a82579704858528 |
| institution | Directory Open Access Journal |
| issn | 2616-4760 |
| language | English |
| last_indexed | 2024-04-11T04:11:01Z |
| publishDate | 2023-04-01 |
| publisher | Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology |
| record_format | Article |
| series | Journal of Advanced Biotechnology and Experimental Therapeutics |
| spelling | doaj.art-b0b4402072ca47e08a825797048585282023-01-01T08:49:41ZengBangladesh Society for Microbiology, Immunology, and Advanced BiotechnologyJournal of Advanced Biotechnology and Experimental Therapeutics2616-47602023-04-0161586610.5455/jabet.2023.d10668559Detection and biological control measures of anthracnose causing fungus isolated from Citrus limon (L.)Md Faruk Hasan0Jannatul Ferdous1Amit Kumar Dutta2Fahmida Begum Mina3Sumon Karmakar4Biswanath Sikdar5Department of Microbiology, University of Rajshahi, Rajshahi-6205, Bangladesh Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh. Department of Microbiology, University of Rajshahi, Rajshahi-6205, Bangladesh. Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh. Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh. Department of Microbiology, University of Rajshahi, Rajshahi-6205, Bangladesh.Anthracnose is one of the most devastating fungi causing twig dieback and postharvest fruit decay. Present study was aimed to identification of anthracnose fungus. Infected leaves of Citrus limon were collected and cultured on potato dextrose agar (PDA) media for pathogenic fungus isolation. The isolated fungal pure culture was characterized by physiological and morphological characterization methods. Biological control measures of the fungus were evaluated by disc diffusion methods. The highest growth and development of isolated fungus was detected in PDA media pH 7 in fructose as the best carbon source and 0.05gm NaCl concentrations at 37°C. Pathogenicity potency of isolate was performed on lemon, orange and malta, belonging to the Citrus sp. For antifungal activity, 200µgm/disc methanolic extract of Psidium guajava showed 14.33±0.66 mm inhibition zone against the isolated fungus. From the present investigations, identified anthracnose causing fungus and it's controlling techniques may help for further research for the isolation of drugs related compound for controlling this disease. [ J Adv Biotechnol Exp Ther 2023; 6(1.000): 58-66]http://www.ejmanager.com/fulltextpdf.php?mno=68559citrus limon; anthracnose; identification; biological control |
| spellingShingle | Md Faruk Hasan Jannatul Ferdous Amit Kumar Dutta Fahmida Begum Mina Sumon Karmakar Biswanath Sikdar Detection and biological control measures of anthracnose causing fungus isolated from Citrus limon (L.) Journal of Advanced Biotechnology and Experimental Therapeutics citrus limon; anthracnose; identification; biological control |
| title | Detection and biological control measures of anthracnose causing fungus isolated from Citrus limon (L.) |
| title_full | Detection and biological control measures of anthracnose causing fungus isolated from Citrus limon (L.) |
| title_fullStr | Detection and biological control measures of anthracnose causing fungus isolated from Citrus limon (L.) |
| title_full_unstemmed | Detection and biological control measures of anthracnose causing fungus isolated from Citrus limon (L.) |
| title_short | Detection and biological control measures of anthracnose causing fungus isolated from Citrus limon (L.) |
| title_sort | detection and biological control measures of anthracnose causing fungus isolated from citrus limon l |
| topic | citrus limon; anthracnose; identification; biological control |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=68559 |
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