Sentinel network for monitoring in vitro susceptibility of Plasmodium falciparum to antimalarial drugs in Colombia: a proof of concept
Drug resistance is one of the principal obstacles blocking worldwide malaria control. In Colombia, malaria remains a major public health concern and drug-resistant parasites have been reported. In vitro drug susceptibility assays are a useful tool for monitoring the emergence and spread of drug-resi...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2011-08-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000900016&lng=en&tlng=en |
| _version_ | 1797756724361297920 |
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| author | Samanda L Aponte Gustavo Díaz Zuleima Pava Diego F Echeverry Darío Ibarguen Melissa Rios Luz M Murcia Claudia Quelal Claribel Murillo Pedro Gil Anders Björkman Lyda Osorio |
| author_facet | Samanda L Aponte Gustavo Díaz Zuleima Pava Diego F Echeverry Darío Ibarguen Melissa Rios Luz M Murcia Claudia Quelal Claribel Murillo Pedro Gil Anders Björkman Lyda Osorio |
| author_sort | Samanda L Aponte |
| collection | DOAJ |
| description | Drug resistance is one of the principal obstacles blocking worldwide malaria control. In Colombia, malaria remains a major public health concern and drug-resistant parasites have been reported. In vitro drug susceptibility assays are a useful tool for monitoring the emergence and spread of drug-resistant Plasmodium falciparum. The present study was conducted as a proof of concept for an antimalarial drug resistance surveillance network based on in vitro susceptibility testing in Colombia. Sentinel laboratories were set up in three malaria endemic areas. The enzyme linked immunosorbent assay-histidine rich protein 2 and schizont maturation methods were used to assess the susceptibility of fresh P. falciparum isolates to six antimalarial drugs. This study demonstrates that an antimalarial drug resistance surveillance network based on in vitro methods is feasible in the field with the participation of a research institute, local health institutions and universities. It could also serve as a model for a regional surveillance network. Preliminary susceptibility results showed widespread chloroquine resistance, which was consistent with previous reports for the Pacific region. However, high susceptibility to dihydroartemisinin and lumefantrine compounds, currently used for treatment in the country, was also reported. The implementation process identified critical points and opportunities for the improvement of network sustainability strategies. |
| first_indexed | 2024-03-12T18:05:39Z |
| format | Article |
| id | doaj.art-b0b52fc05fb141259f049c2689ff3fcb |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:05:39Z |
| publishDate | 2011-08-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-b0b52fc05fb141259f049c2689ff3fcb2023-08-02T09:28:36ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602011-08-01106suppl 112312910.1590/S0074-02762011000900016S0074-02762011000900016Sentinel network for monitoring in vitro susceptibility of Plasmodium falciparum to antimalarial drugs in Colombia: a proof of conceptSamanda L Aponte0Gustavo Díaz1Zuleima Pava2Diego F Echeverry3Darío Ibarguen4Melissa Rios5Luz M Murcia6Claudia Quelal7Claribel Murillo8Pedro Gil9Anders Björkman10Lyda Osorio11Centro Internacional de Entrenamiento en Investigaciones MédicasCentro Internacional de Entrenamiento en Investigaciones MédicasCentro Internacional de Entrenamiento en Investigaciones MédicasCentro Internacional de Entrenamiento en Investigaciones MédicasSecretaría Departamental de Salud de ChocóSecretaría Departamental de Salud de AmazonasSecretaría Departamental de Salud de AmazonasInstituto Departamental de Salud de NariñoCentro Internacional de Entrenamiento en Investigaciones MédicasKarolinska InstitutetKarolinska InstitutetCentro Internacional de Entrenamiento en Investigaciones MédicasDrug resistance is one of the principal obstacles blocking worldwide malaria control. In Colombia, malaria remains a major public health concern and drug-resistant parasites have been reported. In vitro drug susceptibility assays are a useful tool for monitoring the emergence and spread of drug-resistant Plasmodium falciparum. The present study was conducted as a proof of concept for an antimalarial drug resistance surveillance network based on in vitro susceptibility testing in Colombia. Sentinel laboratories were set up in three malaria endemic areas. The enzyme linked immunosorbent assay-histidine rich protein 2 and schizont maturation methods were used to assess the susceptibility of fresh P. falciparum isolates to six antimalarial drugs. This study demonstrates that an antimalarial drug resistance surveillance network based on in vitro methods is feasible in the field with the participation of a research institute, local health institutions and universities. It could also serve as a model for a regional surveillance network. Preliminary susceptibility results showed widespread chloroquine resistance, which was consistent with previous reports for the Pacific region. However, high susceptibility to dihydroartemisinin and lumefantrine compounds, currently used for treatment in the country, was also reported. The implementation process identified critical points and opportunities for the improvement of network sustainability strategies.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000900016&lng=en&tlng=enPlasmodium falciparum malariaantimalarial drugssurveillance |
| spellingShingle | Samanda L Aponte Gustavo Díaz Zuleima Pava Diego F Echeverry Darío Ibarguen Melissa Rios Luz M Murcia Claudia Quelal Claribel Murillo Pedro Gil Anders Björkman Lyda Osorio Sentinel network for monitoring in vitro susceptibility of Plasmodium falciparum to antimalarial drugs in Colombia: a proof of concept Memorias do Instituto Oswaldo Cruz Plasmodium falciparum malaria antimalarial drugs surveillance |
| title | Sentinel network for monitoring in vitro susceptibility of Plasmodium falciparum to antimalarial drugs in Colombia: a proof of concept |
| title_full | Sentinel network for monitoring in vitro susceptibility of Plasmodium falciparum to antimalarial drugs in Colombia: a proof of concept |
| title_fullStr | Sentinel network for monitoring in vitro susceptibility of Plasmodium falciparum to antimalarial drugs in Colombia: a proof of concept |
| title_full_unstemmed | Sentinel network for monitoring in vitro susceptibility of Plasmodium falciparum to antimalarial drugs in Colombia: a proof of concept |
| title_short | Sentinel network for monitoring in vitro susceptibility of Plasmodium falciparum to antimalarial drugs in Colombia: a proof of concept |
| title_sort | sentinel network for monitoring in vitro susceptibility of plasmodium falciparum to antimalarial drugs in colombia a proof of concept |
| topic | Plasmodium falciparum malaria antimalarial drugs surveillance |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000900016&lng=en&tlng=en |
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