Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma
In light of PRP’s increasing popularity in veterinary practice, this study aimed to compare three manual methods to prepare and cool equine PRP. The blood of 18 clinically healthy mares was collected via venipuncture in a blood transfusion bag (method 1), blood tubes (method 2), and a syringe (metho...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/6/1478 |
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| author | Lorenzo G. T. M. Segabinazzi Giorgia Podico Michael F. Rosser Som G. Nanjappa Marco A. Alvarenga Igor F. Canisso |
| author_facet | Lorenzo G. T. M. Segabinazzi Giorgia Podico Michael F. Rosser Som G. Nanjappa Marco A. Alvarenga Igor F. Canisso |
| author_sort | Lorenzo G. T. M. Segabinazzi |
| collection | DOAJ |
| description | In light of PRP’s increasing popularity in veterinary practice, this study aimed to compare three manual methods to prepare and cool equine PRP. The blood of 18 clinically healthy mares was collected via venipuncture in a blood transfusion bag (method 1), blood tubes (method 2), and a syringe (method 3). In method 1, samples were double centrifuged; method 2 involved one centrifugation, and in method 3 the syringe was kept in an upright position to sediment for 4 h. After processing with three methods, PRP and platelet-poor plasma (PPP) were extracted and assessed for red (RBC) and white blood cell counts (WBC), platelet counts, and viability. In a subset of mares (<i>n</i> = 6), samples were processed with the three methods, and PRP was evaluated at 6 and 24 h postcooling at 5 °C. Method 1 resulted in the highest and method 3 in the lowest platelet concentration (<i>p</i> < 0.05), and the latter also had greater contamination with WBC than the others (<i>p</i> < 0.001). Platelet viability was similar across treatments (<i>p</i> > 0.05). Cooling for 24 h did not affect platelet counts in all methods (<i>p</i> > 0.05); however, platelet viability was reduced after cooling PRP produced by method 3 (<i>p</i> = 0.04), and agglutination increased over time in all methods (<i>p</i> < 0.001). The three methods increased (1.8–5.6-fold) platelet concentration in PRP compared to whole blood without compromising platelet viability. In conclusion, all three methods concentrated platelets and while cooling affected their viability. It remains unknown whether the different methods and cooling would affect PRP’s clinical efficacy. |
| first_indexed | 2024-03-10T11:12:31Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T11:12:31Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-b0b8ef77cd2f4d16b7fb95debbb55ae02023-11-21T20:41:08ZengMDPI AGAnimals2076-26152021-05-01116147810.3390/ani11061478Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich PlasmaLorenzo G. T. M. Segabinazzi0Giorgia Podico1Michael F. Rosser2Som G. Nanjappa3Marco A. Alvarenga4Igor F. Canisso5Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana Champaign, Urbana, IL 61802, USADepartment of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana Champaign, Urbana, IL 61802, USADepartment of Pathobiology, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL 61802, USADepartment of Pathobiology, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL 61802, USASchool of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18618681, SP, BrazilDepartment of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana Champaign, Urbana, IL 61802, USAIn light of PRP’s increasing popularity in veterinary practice, this study aimed to compare three manual methods to prepare and cool equine PRP. The blood of 18 clinically healthy mares was collected via venipuncture in a blood transfusion bag (method 1), blood tubes (method 2), and a syringe (method 3). In method 1, samples were double centrifuged; method 2 involved one centrifugation, and in method 3 the syringe was kept in an upright position to sediment for 4 h. After processing with three methods, PRP and platelet-poor plasma (PPP) were extracted and assessed for red (RBC) and white blood cell counts (WBC), platelet counts, and viability. In a subset of mares (<i>n</i> = 6), samples were processed with the three methods, and PRP was evaluated at 6 and 24 h postcooling at 5 °C. Method 1 resulted in the highest and method 3 in the lowest platelet concentration (<i>p</i> < 0.05), and the latter also had greater contamination with WBC than the others (<i>p</i> < 0.001). Platelet viability was similar across treatments (<i>p</i> > 0.05). Cooling for 24 h did not affect platelet counts in all methods (<i>p</i> > 0.05); however, platelet viability was reduced after cooling PRP produced by method 3 (<i>p</i> = 0.04), and agglutination increased over time in all methods (<i>p</i> < 0.001). The three methods increased (1.8–5.6-fold) platelet concentration in PRP compared to whole blood without compromising platelet viability. In conclusion, all three methods concentrated platelets and while cooling affected their viability. It remains unknown whether the different methods and cooling would affect PRP’s clinical efficacy.https://www.mdpi.com/2076-2615/11/6/1478platelet concentratehorseendometritisblood byproductplatelet viability |
| spellingShingle | Lorenzo G. T. M. Segabinazzi Giorgia Podico Michael F. Rosser Som G. Nanjappa Marco A. Alvarenga Igor F. Canisso Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma Animals platelet concentrate horse endometritis blood byproduct platelet viability |
| title | Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma |
| title_full | Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma |
| title_fullStr | Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma |
| title_full_unstemmed | Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma |
| title_short | Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma |
| title_sort | three manual noncommercial methods to prepare equine platelet rich plasma |
| topic | platelet concentrate horse endometritis blood byproduct platelet viability |
| url | https://www.mdpi.com/2076-2615/11/6/1478 |
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