Combined Use of eDNA Metabarcoding and Bottom Trawling for the Assessment of Fish Biodiversity in the Zhoushan Sea
With the increase in fishing intensity and the intensification of marine pollution, the fishery resources in the Zhoushan Sea are seriously degraded, and the difficulty of censusing fish diversity hampers effective management in marine fishes. Environmental DNA metabarcoding and bottom trawl methods...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2021.809703/full |
| _version_ | 1798024821230010368 |
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| author | Shan Zhou Shan Zhou Shan Zhou Chenrong Fan Chenrong Fan Chenrong Fan Haoming Xia Jian Zhang Wei Yang Wei Yang Wei Yang Dengjie Ji Dengjie Ji Dengjie Ji Lei Wang Lei Wang Lei Wang Li Chen Li Chen Li Chen Nannan Liu Nannan Liu Nannan Liu |
| author_facet | Shan Zhou Shan Zhou Shan Zhou Chenrong Fan Chenrong Fan Chenrong Fan Haoming Xia Jian Zhang Wei Yang Wei Yang Wei Yang Dengjie Ji Dengjie Ji Dengjie Ji Lei Wang Lei Wang Lei Wang Li Chen Li Chen Li Chen Nannan Liu Nannan Liu Nannan Liu |
| author_sort | Shan Zhou |
| collection | DOAJ |
| description | With the increase in fishing intensity and the intensification of marine pollution, the fishery resources in the Zhoushan Sea are seriously degraded, and the difficulty of censusing fish diversity hampers effective management in marine fishes. Environmental DNA metabarcoding and bottom trawl methods were used to determine the ability of the methods to distinguish fish assemblages in the Zhoushan Sea. The species composition and diversity of the Zhoushan Sea were assessed via high-throughput sequencing analysis of eDNA coupled with bottom trawl fishery survey data, after which the two methods were compared. eDNA screening identified 38.2% more fish species than bottom trawls. Combining these two methods, 33 orders, 65 families, and 130 species of fishes were identified. Perciformes and Clupeiformes, the most abundant orders in the catch, represented 31.5 and 10.0% of the total fish abundance, respectively. The results of ANOSIM and redundancy analyses indicated that the fish community structure varied significantly between summer and winter, however depth and temperature being the main environmental factors influencing fish distribution. The biodiversity index was higher in summer than in winter. Thus, our work provides more detailed seasonal data on biodiversity in the Zhoushan Sea, which is essential for the long-term management and conservation of coastal biodiversity. Compared with traditional survey methods, eDNA determination is highly sensitive, accurate, cost-efficient, and suitable for fish diversity studies in relevant sea areas. Although this approach cannot completely replace traditional methods, our findings demonstrate that it provides a reliable complementary method for assessing fish diversity in marine ecosystems. |
| first_indexed | 2024-04-11T18:09:02Z |
| format | Article |
| id | doaj.art-b0b99ca415bf431da9d8cf9b86717247 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-11T18:09:02Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-b0b99ca415bf431da9d8cf9b867172472022-12-22T04:10:14ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-01-01810.3389/fmars.2021.809703809703Combined Use of eDNA Metabarcoding and Bottom Trawling for the Assessment of Fish Biodiversity in the Zhoushan SeaShan Zhou0Shan Zhou1Shan Zhou2Chenrong Fan3Chenrong Fan4Chenrong Fan5Haoming Xia6Jian Zhang7Wei Yang8Wei Yang9Wei Yang10Dengjie Ji11Dengjie Ji12Dengjie Ji13Lei Wang14Lei Wang15Lei Wang16Li Chen17Li Chen18Li Chen19Nannan Liu20Nannan Liu21Nannan Liu22Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, ChinaCo-innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Institute of Marine Resources Development, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, ChinaCo-innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Institute of Marine Resources Development, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Institute of Marine Resources Development, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, ChinaCo-innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Institute of Marine Resources Development, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, ChinaCo-innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Institute of Marine Resources Development, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, ChinaCo-innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Institute of Marine Resources Development, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, ChinaCo-innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Institute of Marine Resources Development, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, ChinaCo-innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Institute of Marine Resources Development, Jiangsu Ocean University, Lianyungang, ChinaWith the increase in fishing intensity and the intensification of marine pollution, the fishery resources in the Zhoushan Sea are seriously degraded, and the difficulty of censusing fish diversity hampers effective management in marine fishes. Environmental DNA metabarcoding and bottom trawl methods were used to determine the ability of the methods to distinguish fish assemblages in the Zhoushan Sea. The species composition and diversity of the Zhoushan Sea were assessed via high-throughput sequencing analysis of eDNA coupled with bottom trawl fishery survey data, after which the two methods were compared. eDNA screening identified 38.2% more fish species than bottom trawls. Combining these two methods, 33 orders, 65 families, and 130 species of fishes were identified. Perciformes and Clupeiformes, the most abundant orders in the catch, represented 31.5 and 10.0% of the total fish abundance, respectively. The results of ANOSIM and redundancy analyses indicated that the fish community structure varied significantly between summer and winter, however depth and temperature being the main environmental factors influencing fish distribution. The biodiversity index was higher in summer than in winter. Thus, our work provides more detailed seasonal data on biodiversity in the Zhoushan Sea, which is essential for the long-term management and conservation of coastal biodiversity. Compared with traditional survey methods, eDNA determination is highly sensitive, accurate, cost-efficient, and suitable for fish diversity studies in relevant sea areas. Although this approach cannot completely replace traditional methods, our findings demonstrate that it provides a reliable complementary method for assessing fish diversity in marine ecosystems.https://www.frontiersin.org/articles/10.3389/fmars.2021.809703/fullenvironmental DNAfish diversityenvironmental factorsbottom trawl fisheryZhoushan Sea |
| spellingShingle | Shan Zhou Shan Zhou Shan Zhou Chenrong Fan Chenrong Fan Chenrong Fan Haoming Xia Jian Zhang Wei Yang Wei Yang Wei Yang Dengjie Ji Dengjie Ji Dengjie Ji Lei Wang Lei Wang Lei Wang Li Chen Li Chen Li Chen Nannan Liu Nannan Liu Nannan Liu Combined Use of eDNA Metabarcoding and Bottom Trawling for the Assessment of Fish Biodiversity in the Zhoushan Sea Frontiers in Marine Science environmental DNA fish diversity environmental factors bottom trawl fishery Zhoushan Sea |
| title | Combined Use of eDNA Metabarcoding and Bottom Trawling for the Assessment of Fish Biodiversity in the Zhoushan Sea |
| title_full | Combined Use of eDNA Metabarcoding and Bottom Trawling for the Assessment of Fish Biodiversity in the Zhoushan Sea |
| title_fullStr | Combined Use of eDNA Metabarcoding and Bottom Trawling for the Assessment of Fish Biodiversity in the Zhoushan Sea |
| title_full_unstemmed | Combined Use of eDNA Metabarcoding and Bottom Trawling for the Assessment of Fish Biodiversity in the Zhoushan Sea |
| title_short | Combined Use of eDNA Metabarcoding and Bottom Trawling for the Assessment of Fish Biodiversity in the Zhoushan Sea |
| title_sort | combined use of edna metabarcoding and bottom trawling for the assessment of fish biodiversity in the zhoushan sea |
| topic | environmental DNA fish diversity environmental factors bottom trawl fishery Zhoushan Sea |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2021.809703/full |
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