Ethylene is involved in pistil fate by modulating the onset of ovule senescence and the GA-mediated fruit set in <it>Arabidopsis</it>
<p>Abstract</p> <p>Background</p> <p>Ovule lifespan is an important factor in determining the ability to set fruits and produce seeds. Once ovule senescence is established, fruit set capacity in response to gibberellins (GAs) is lost. We aimed to elucidate whether ethyl...
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| Format: | Article |
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BMC
2011-05-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/11/84 |
| _version_ | 1831629894138724352 |
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| author | Carbonell Juan Granell Antonio Urbez Cristina Carbonell-Bejerano Pablo Perez-Amador Miguel A |
| author_facet | Carbonell Juan Granell Antonio Urbez Cristina Carbonell-Bejerano Pablo Perez-Amador Miguel A |
| author_sort | Carbonell Juan |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Ovule lifespan is an important factor in determining the ability to set fruits and produce seeds. Once ovule senescence is established, fruit set capacity in response to gibberellins (GAs) is lost. We aimed to elucidate whether ethylene plays a role in controlling ovule senescence and the fruit set response in <it>Arabidopsis</it>.</p> <p>Results</p> <p>Ethylene response inhibitors, silver thiosulphate (STS) and 1-methylcyclopropene (1-MCP), were able to delay the loss of pistil response to GA<sub>3</sub>. In addition, ethylene insensitive mutants <it>ein2-5 </it>and <it>ein3-1 </it>showed delayed loss of pistil response, as in plants treated with STS and 1-MCP, while constitutive mutant <it>ctr1-1 </it>displayed premature loss of response. The analysis of the expression of ethylene biosynthesis genes suggests that ethylene is synthesised in ovules at the onset of ovule senescence, while a transcriptional meta-analysis also supports an activated ethylene-dependent senescence upon the establishment of ovule senescence. Finally, a <it>SAG12:GUS </it>reporter line proved useful to monitor ovule senescence and to directly demonstrate that ethylene specifically modulates ovule senescence.</p> <p>Conclusions</p> <p>We have shown that ethylene is involved in both the control of the ovule lifespan and the determination of the pistil/fruit fate. Our data support a role of the ovule in modulating the GA response during fruit set in <it>Arabidopsis</it>. A possible mechanism that links the ethylene modulation of the ovule senescence and the GA<sub>3</sub>-induced fruit set response is discussed.</p> |
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| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-19T02:08:13Z |
| publishDate | 2011-05-01 |
| publisher | BMC |
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| series | BMC Plant Biology |
| spelling | doaj.art-b0baf0f510984dda86538a8eb0f8f04f2022-12-21T20:40:53ZengBMCBMC Plant Biology1471-22292011-05-011118410.1186/1471-2229-11-84Ethylene is involved in pistil fate by modulating the onset of ovule senescence and the GA-mediated fruit set in <it>Arabidopsis</it>Carbonell JuanGranell AntonioUrbez CristinaCarbonell-Bejerano PabloPerez-Amador Miguel A<p>Abstract</p> <p>Background</p> <p>Ovule lifespan is an important factor in determining the ability to set fruits and produce seeds. Once ovule senescence is established, fruit set capacity in response to gibberellins (GAs) is lost. We aimed to elucidate whether ethylene plays a role in controlling ovule senescence and the fruit set response in <it>Arabidopsis</it>.</p> <p>Results</p> <p>Ethylene response inhibitors, silver thiosulphate (STS) and 1-methylcyclopropene (1-MCP), were able to delay the loss of pistil response to GA<sub>3</sub>. In addition, ethylene insensitive mutants <it>ein2-5 </it>and <it>ein3-1 </it>showed delayed loss of pistil response, as in plants treated with STS and 1-MCP, while constitutive mutant <it>ctr1-1 </it>displayed premature loss of response. The analysis of the expression of ethylene biosynthesis genes suggests that ethylene is synthesised in ovules at the onset of ovule senescence, while a transcriptional meta-analysis also supports an activated ethylene-dependent senescence upon the establishment of ovule senescence. Finally, a <it>SAG12:GUS </it>reporter line proved useful to monitor ovule senescence and to directly demonstrate that ethylene specifically modulates ovule senescence.</p> <p>Conclusions</p> <p>We have shown that ethylene is involved in both the control of the ovule lifespan and the determination of the pistil/fruit fate. Our data support a role of the ovule in modulating the GA response during fruit set in <it>Arabidopsis</it>. A possible mechanism that links the ethylene modulation of the ovule senescence and the GA<sub>3</sub>-induced fruit set response is discussed.</p>http://www.biomedcentral.com/1471-2229/11/84 |
| spellingShingle | Carbonell Juan Granell Antonio Urbez Cristina Carbonell-Bejerano Pablo Perez-Amador Miguel A Ethylene is involved in pistil fate by modulating the onset of ovule senescence and the GA-mediated fruit set in <it>Arabidopsis</it> BMC Plant Biology |
| title | Ethylene is involved in pistil fate by modulating the onset of ovule senescence and the GA-mediated fruit set in <it>Arabidopsis</it> |
| title_full | Ethylene is involved in pistil fate by modulating the onset of ovule senescence and the GA-mediated fruit set in <it>Arabidopsis</it> |
| title_fullStr | Ethylene is involved in pistil fate by modulating the onset of ovule senescence and the GA-mediated fruit set in <it>Arabidopsis</it> |
| title_full_unstemmed | Ethylene is involved in pistil fate by modulating the onset of ovule senescence and the GA-mediated fruit set in <it>Arabidopsis</it> |
| title_short | Ethylene is involved in pistil fate by modulating the onset of ovule senescence and the GA-mediated fruit set in <it>Arabidopsis</it> |
| title_sort | ethylene is involved in pistil fate by modulating the onset of ovule senescence and the ga mediated fruit set in it arabidopsis it |
| url | http://www.biomedcentral.com/1471-2229/11/84 |
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