Comparative study of five commercial probes for the detection of Epstein-Barr virus (EBV) by in situ hybridization in cases of nodular sclerosis Hodgki's lymphoma
ABSTRACT Introduction: Epstein-Barr virus (EBV) may serve as a target in therapeutic treatments, thus reliable diagnostic results are necessary. Objective: The aim of this study was to evaluate the accuracy of EBV detection by in situ hybridization (ISH) using five commercial probes in formalin-fi...
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Sociedade Brasileira de Patologia Clínica
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| Series: | Jornal Brasileiro de Patologia e Medicina Laboratorial |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442016000600416&lng=en&tlng=en |
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| author | Suely Nonogaki Neuza K. Shirata Lidia M. Kimura Juliana M. Guerra Raphael S. S. Medeiros Roberto Antonio P. Paes Cristina T. Kanamura Camila C. Oliveira Yara de Menezes |
| author_facet | Suely Nonogaki Neuza K. Shirata Lidia M. Kimura Juliana M. Guerra Raphael S. S. Medeiros Roberto Antonio P. Paes Cristina T. Kanamura Camila C. Oliveira Yara de Menezes |
| author_sort | Suely Nonogaki |
| collection | DOAJ |
| description | ABSTRACT Introduction: Epstein-Barr virus (EBV) may serve as a target in therapeutic treatments, thus reliable diagnostic results are necessary. Objective: The aim of this study was to evaluate the accuracy of EBV detection by in situ hybridization (ISH) using five commercial probes in formalin-fixed and paraffin-embedded samples of nodular sclerosis Hodgkin's lymphoma (HL), and to compare the results with immunohistochemistry (IHC) and polymerase chain reaction (PCR). Material and method: Thirty samples were selected, 28 were lymph nodes, one bone marrow and one mediastinum. The following parameters were analyzed: signal intensity; proportionality of positive cells; quality of the reaction according to comfort for evaluation, sign quality and homogeneity of labeled cells; background reaction; morphology; presence of artifacts; and positivity in other non-neoplastic cells. All samples were analyzed for EBV detection using the five probes, IHC for latent membrane protein type 1 (LMP1) and PCR for Epstein Barr virus nuclear antigen 1 (EBNA1). Statistical analyses were performed with the R1 software; Fleiss' test and Cohen Kappa index of 5% were considered significant. Results: The detection by IHC-LMP1 was 26.7% (8/30) and 66.7% (20/30) by PCR-EBNA1. All probes detected EBV. Positivity was observed in 42/90 (46.7%), 38/90 (42.2%), 45/90 (50%), 27/90 (30%) and 61/90 (67.8%) for probes A, B, C, D and E, respectively. Discussion: All five probes demonstrated positivity. Conclusion: Probe E showed better rate (67.8%), sensitivity, specificity and accuracy (100%), a very good correlation among the different observers and with PCR, besides great cost-benefits relation. |
| first_indexed | 2024-12-12T05:08:58Z |
| format | Article |
| id | doaj.art-b0bb99e3f4d04ae195f9e7314d0baaca |
| institution | Directory Open Access Journal |
| issn | 1676-2444 1678-4774 |
| language | English |
| last_indexed | 2024-12-12T05:08:58Z |
| publisher | Sociedade Brasileira de Patologia Clínica |
| record_format | Article |
| series | Jornal Brasileiro de Patologia e Medicina Laboratorial |
| spelling | doaj.art-b0bb99e3f4d04ae195f9e7314d0baaca2022-12-22T00:37:01ZengSociedade Brasileira de Patologia ClínicaJornal Brasileiro de Patologia e Medicina Laboratorial1676-24441678-477452641642510.5935/1676-2444.20160065S1676-24442016000600416Comparative study of five commercial probes for the detection of Epstein-Barr virus (EBV) by in situ hybridization in cases of nodular sclerosis Hodgki's lymphomaSuely NonogakiNeuza K. ShirataLidia M. KimuraJuliana M. GuerraRaphael S. S. MedeirosRoberto Antonio P. PaesCristina T. KanamuraCamila C. OliveiraYara de MenezesABSTRACT Introduction: Epstein-Barr virus (EBV) may serve as a target in therapeutic treatments, thus reliable diagnostic results are necessary. Objective: The aim of this study was to evaluate the accuracy of EBV detection by in situ hybridization (ISH) using five commercial probes in formalin-fixed and paraffin-embedded samples of nodular sclerosis Hodgkin's lymphoma (HL), and to compare the results with immunohistochemistry (IHC) and polymerase chain reaction (PCR). Material and method: Thirty samples were selected, 28 were lymph nodes, one bone marrow and one mediastinum. The following parameters were analyzed: signal intensity; proportionality of positive cells; quality of the reaction according to comfort for evaluation, sign quality and homogeneity of labeled cells; background reaction; morphology; presence of artifacts; and positivity in other non-neoplastic cells. All samples were analyzed for EBV detection using the five probes, IHC for latent membrane protein type 1 (LMP1) and PCR for Epstein Barr virus nuclear antigen 1 (EBNA1). Statistical analyses were performed with the R1 software; Fleiss' test and Cohen Kappa index of 5% were considered significant. Results: The detection by IHC-LMP1 was 26.7% (8/30) and 66.7% (20/30) by PCR-EBNA1. All probes detected EBV. Positivity was observed in 42/90 (46.7%), 38/90 (42.2%), 45/90 (50%), 27/90 (30%) and 61/90 (67.8%) for probes A, B, C, D and E, respectively. Discussion: All five probes demonstrated positivity. Conclusion: Probe E showed better rate (67.8%), sensitivity, specificity and accuracy (100%), a very good correlation among the different observers and with PCR, besides great cost-benefits relation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442016000600416&lng=en&tlng=eninfecções pelo vírus epstein-barrhibridização in situdoença de hodgkin |
| spellingShingle | Suely Nonogaki Neuza K. Shirata Lidia M. Kimura Juliana M. Guerra Raphael S. S. Medeiros Roberto Antonio P. Paes Cristina T. Kanamura Camila C. Oliveira Yara de Menezes Comparative study of five commercial probes for the detection of Epstein-Barr virus (EBV) by in situ hybridization in cases of nodular sclerosis Hodgki's lymphoma Jornal Brasileiro de Patologia e Medicina Laboratorial infecções pelo vírus epstein-barr hibridização in situ doença de hodgkin |
| title | Comparative study of five commercial probes for the detection of Epstein-Barr virus (EBV) by in situ hybridization in cases of nodular sclerosis Hodgki's lymphoma |
| title_full | Comparative study of five commercial probes for the detection of Epstein-Barr virus (EBV) by in situ hybridization in cases of nodular sclerosis Hodgki's lymphoma |
| title_fullStr | Comparative study of five commercial probes for the detection of Epstein-Barr virus (EBV) by in situ hybridization in cases of nodular sclerosis Hodgki's lymphoma |
| title_full_unstemmed | Comparative study of five commercial probes for the detection of Epstein-Barr virus (EBV) by in situ hybridization in cases of nodular sclerosis Hodgki's lymphoma |
| title_short | Comparative study of five commercial probes for the detection of Epstein-Barr virus (EBV) by in situ hybridization in cases of nodular sclerosis Hodgki's lymphoma |
| title_sort | comparative study of five commercial probes for the detection of epstein barr virus ebv by in situ hybridization in cases of nodular sclerosis hodgki s lymphoma |
| topic | infecções pelo vírus epstein-barr hibridização in situ doença de hodgkin |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442016000600416&lng=en&tlng=en |
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