Microbial Sampling Using Interdental Brushes and Paper Points around Teeth and Implants: A Pilot Study for Comparison
Inflammatory periodontal and peri-implant diseases follow dysbiotic shifts in a susceptible host. A well-established tool for microbial sample collection is the use of paper points. The purpose of this pilot study was to evaluate the use of interdental brushes compared to paper points. Biofilm sampl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/13/6/1054 |
| _version_ | 1797612427450253312 |
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| author | Tobias M. Janson Yann Gager Christian R. Hatz Anne-Katrin Köhler Stefanie J. Gartenmann Patrick R. Schmidlin |
| author_facet | Tobias M. Janson Yann Gager Christian R. Hatz Anne-Katrin Köhler Stefanie J. Gartenmann Patrick R. Schmidlin |
| author_sort | Tobias M. Janson |
| collection | DOAJ |
| description | Inflammatory periodontal and peri-implant diseases follow dysbiotic shifts in a susceptible host. A well-established tool for microbial sample collection is the use of paper points. The purpose of this pilot study was to evaluate the use of interdental brushes compared to paper points. Biofilm samples were collected with paper points and later interdental brushes from ten patients. Five patients were represented with a community periodontal index of treatment needs (CPITN) of 0–2 around the teeth and an implant with PPD ≤ 5 mm and no radiological bone loss. The remaining five patients had a CPITN ≥ 3 and one implant with peri-implantitis. Microbial samples were analyzed with quantitative polymerase chain reaction (qPCR) and next-generation sequencing (NGS). The results showed higher amounts of DNA in samples taken by interdental brushes but also higher Ct values. Both methods detected <i>Filifactor alocis</i>, <i>Porphyromonas gingivalis</i>, <i>Prevotella intermedia</i>, <i>Tannerella forsythia,</i> and <i>Treponema denticola</i> in the majority of samples, while <i>Aggregatibacter actinomycetemcomitans</i> was rarely found. A microbial dysbiosis index showed comparable or higher values in sites with no periodontitis/peri-implantitis with interdental brushes. The results of this pilot study indicate that interdental brushes might be a valid technique for microbial sampling and particularly advantageous in the early detection of dysbiotic shifts around teeth and implants. Larger studies with more participants are needed to validate the proposed microbial sampling method with interdental brushes. |
| first_indexed | 2024-03-11T06:42:06Z |
| format | Article |
| id | doaj.art-b0bdb0880c1d4116a57eaeb5e971202b |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-11T06:42:06Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-b0bdb0880c1d4116a57eaeb5e971202b2023-11-17T10:33:42ZengMDPI AGDiagnostics2075-44182023-03-01136105410.3390/diagnostics13061054Microbial Sampling Using Interdental Brushes and Paper Points around Teeth and Implants: A Pilot Study for ComparisonTobias M. Janson0Yann Gager1Christian R. Hatz2Anne-Katrin Köhler3Stefanie J. Gartenmann4Patrick R. Schmidlin5Clinic of Conservative & Preventive Dentistry, Division of Periodontology & Peri-Implant Diseases, Center of Dental Medicine, University of Zurich, 8032 Zurich, SwitzerlandParoX Dental Gmbh, 04103 Leipzig, GermanyClinic of Conservative & Preventive Dentistry, Division of Periodontology & Peri-Implant Diseases, Center of Dental Medicine, University of Zurich, 8032 Zurich, SwitzerlandParoX Dental Gmbh, 04103 Leipzig, GermanyDr. Stefanie Gartenmann Dental Practice, 6330 Cham, SwitzerlandClinic of Conservative & Preventive Dentistry, Division of Periodontology & Peri-Implant Diseases, Center of Dental Medicine, University of Zurich, 8032 Zurich, SwitzerlandInflammatory periodontal and peri-implant diseases follow dysbiotic shifts in a susceptible host. A well-established tool for microbial sample collection is the use of paper points. The purpose of this pilot study was to evaluate the use of interdental brushes compared to paper points. Biofilm samples were collected with paper points and later interdental brushes from ten patients. Five patients were represented with a community periodontal index of treatment needs (CPITN) of 0–2 around the teeth and an implant with PPD ≤ 5 mm and no radiological bone loss. The remaining five patients had a CPITN ≥ 3 and one implant with peri-implantitis. Microbial samples were analyzed with quantitative polymerase chain reaction (qPCR) and next-generation sequencing (NGS). The results showed higher amounts of DNA in samples taken by interdental brushes but also higher Ct values. Both methods detected <i>Filifactor alocis</i>, <i>Porphyromonas gingivalis</i>, <i>Prevotella intermedia</i>, <i>Tannerella forsythia,</i> and <i>Treponema denticola</i> in the majority of samples, while <i>Aggregatibacter actinomycetemcomitans</i> was rarely found. A microbial dysbiosis index showed comparable or higher values in sites with no periodontitis/peri-implantitis with interdental brushes. The results of this pilot study indicate that interdental brushes might be a valid technique for microbial sampling and particularly advantageous in the early detection of dysbiotic shifts around teeth and implants. Larger studies with more participants are needed to validate the proposed microbial sampling method with interdental brushes.https://www.mdpi.com/2075-4418/13/6/1054periodontitisperi-implantitismicrobial samplinginterdental cleaningmolecular genetics |
| spellingShingle | Tobias M. Janson Yann Gager Christian R. Hatz Anne-Katrin Köhler Stefanie J. Gartenmann Patrick R. Schmidlin Microbial Sampling Using Interdental Brushes and Paper Points around Teeth and Implants: A Pilot Study for Comparison Diagnostics periodontitis peri-implantitis microbial sampling interdental cleaning molecular genetics |
| title | Microbial Sampling Using Interdental Brushes and Paper Points around Teeth and Implants: A Pilot Study for Comparison |
| title_full | Microbial Sampling Using Interdental Brushes and Paper Points around Teeth and Implants: A Pilot Study for Comparison |
| title_fullStr | Microbial Sampling Using Interdental Brushes and Paper Points around Teeth and Implants: A Pilot Study for Comparison |
| title_full_unstemmed | Microbial Sampling Using Interdental Brushes and Paper Points around Teeth and Implants: A Pilot Study for Comparison |
| title_short | Microbial Sampling Using Interdental Brushes and Paper Points around Teeth and Implants: A Pilot Study for Comparison |
| title_sort | microbial sampling using interdental brushes and paper points around teeth and implants a pilot study for comparison |
| topic | periodontitis peri-implantitis microbial sampling interdental cleaning molecular genetics |
| url | https://www.mdpi.com/2075-4418/13/6/1054 |
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