Identification of Transcriptomic Differences between Lower Extremities Arterial Disease, Abdominal Aortic Aneurysm and Chronic Venous Disease in Peripheral Blood Mononuclear Cells Specimens
Several human tissues are investigated in studies of molecular biomarkers associated with diseases development. Special attention is focused on the blood and its components due to combining abundant information about systemic responses to pathological processes as well as high accessibility. In the...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/6/3200 |
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| author | Daniel P. Zalewski Karol P. Ruszel Andrzej Stępniewski Dariusz Gałkowski Jacek Bogucki Przemysław Kołodziej Jolanta Szymańska Bartosz J. Płachno Tomasz Zubilewicz Marcin Feldo Janusz Kocki Anna Bogucka-Kocka |
| author_facet | Daniel P. Zalewski Karol P. Ruszel Andrzej Stępniewski Dariusz Gałkowski Jacek Bogucki Przemysław Kołodziej Jolanta Szymańska Bartosz J. Płachno Tomasz Zubilewicz Marcin Feldo Janusz Kocki Anna Bogucka-Kocka |
| author_sort | Daniel P. Zalewski |
| collection | DOAJ |
| description | Several human tissues are investigated in studies of molecular biomarkers associated with diseases development. Special attention is focused on the blood and its components due to combining abundant information about systemic responses to pathological processes as well as high accessibility. In the current study, transcriptome profiles of peripheral blood mononuclear cells (PBMCs) were used to compare differentially expressed genes between patients with lower extremities arterial disease (LEAD), abdominal aortic aneurysm (AAA) and chronic venous disease (CVD). Gene expression patterns were generated using the Ion S5XL next-generation sequencing platform and were analyzed using DESeq2 and UVE-PLS methods implemented in R programming software. In direct pairwise analysis, 21, 58 and 10 differentially expressed genes were selected from the comparison of LEAD vs. AAA, LEAD vs. CVD and AAA vs. CVD patient groups, respectively. Relationships between expression of dysregulated genes and age, body mass index, creatinine levels, hypertension and medication were identified using Spearman rank correlation test and two-sided Mann–Whitney U test. The functional analysis, performed using DAVID website tool, provides potential implications of selected genes in pathological processes underlying diseases studied. Presented research provides new insight into differences of pathogenesis in LEAD, AAA and CVD, and selected genes could be considered as potential candidates for biomarkers useful in diagnosis and differentiation of studied diseases. |
| first_indexed | 2024-03-10T13:02:16Z |
| format | Article |
| id | doaj.art-b0bff4ae913c4ff384380026728b55ea |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T13:02:16Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b0bff4ae913c4ff384380026728b55ea2023-11-21T11:26:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01226320010.3390/ijms22063200Identification of Transcriptomic Differences between Lower Extremities Arterial Disease, Abdominal Aortic Aneurysm and Chronic Venous Disease in Peripheral Blood Mononuclear Cells SpecimensDaniel P. Zalewski0Karol P. Ruszel1Andrzej Stępniewski2Dariusz Gałkowski3Jacek Bogucki4Przemysław Kołodziej5Jolanta Szymańska6Bartosz J. Płachno7Tomasz Zubilewicz8Marcin Feldo9Janusz Kocki10Anna Bogucka-Kocka11Chair and Department of Biology and Genetics, Medical University of Lublin, 4a Chodźki St., 20-093 Lublin, PolandChair of Medical Genetics, Department of Clinical Genetics, Medical University of Lublin, 11 Radziwiłłowska St., 20-080 Lublin, PolandEcotech Complex Analytical and Programme Centre for Advanced Environmentally Friendly Technologies, University of Marie Curie-Skłodowska, 39 Głęboka St., 20-612 Lublin, PolandDepartment of Pathology and Laboratory Medicine, Rutgers-Robert Wood Johnson Medical School, One Robert Wood Johnson Place, New Brunswick, NJ 08903-0019, USAChair and Department of Organic Chemistry, Medical University of Lublin, 4a Chodźki St., 20-093 Lublin, PolandLaboratory of Diagnostic Parasitology, Chair and Department of Biology and Genetics, Medical University of Lublin, 4a Chodźki St., 20-093 Lublin, PolandDepartment of Integrated Paediatric Dentistry, Chair of Integrated Dentistry, Medical University of Lublin, 6 Chodźki St., 20-093 Lublin, PolandDepartment of Plant Cytology and Embryology, Institute of Botany, Faculty of Biology, Jagiellonian University in Kraków, 9 Gronostajowa St., 30-387 Cracow, PolandChair and Department of Vascular Surgery and Angiology, Medical University of Lublin, 11 Staszica St., 20-081 Lublin, PolandChair and Department of Vascular Surgery and Angiology, Medical University of Lublin, 11 Staszica St., 20-081 Lublin, PolandChair of Medical Genetics, Department of Clinical Genetics, Medical University of Lublin, 11 Radziwiłłowska St., 20-080 Lublin, PolandChair and Department of Biology and Genetics, Medical University of Lublin, 4a Chodźki St., 20-093 Lublin, PolandSeveral human tissues are investigated in studies of molecular biomarkers associated with diseases development. Special attention is focused on the blood and its components due to combining abundant information about systemic responses to pathological processes as well as high accessibility. In the current study, transcriptome profiles of peripheral blood mononuclear cells (PBMCs) were used to compare differentially expressed genes between patients with lower extremities arterial disease (LEAD), abdominal aortic aneurysm (AAA) and chronic venous disease (CVD). Gene expression patterns were generated using the Ion S5XL next-generation sequencing platform and were analyzed using DESeq2 and UVE-PLS methods implemented in R programming software. In direct pairwise analysis, 21, 58 and 10 differentially expressed genes were selected from the comparison of LEAD vs. AAA, LEAD vs. CVD and AAA vs. CVD patient groups, respectively. Relationships between expression of dysregulated genes and age, body mass index, creatinine levels, hypertension and medication were identified using Spearman rank correlation test and two-sided Mann–Whitney U test. The functional analysis, performed using DAVID website tool, provides potential implications of selected genes in pathological processes underlying diseases studied. Presented research provides new insight into differences of pathogenesis in LEAD, AAA and CVD, and selected genes could be considered as potential candidates for biomarkers useful in diagnosis and differentiation of studied diseases.https://www.mdpi.com/1422-0067/22/6/3200lower extremities arterial diseasechronic venous diseaseabdominal aortic aneurysmgene expressionnext generation sequencingbiomarker |
| spellingShingle | Daniel P. Zalewski Karol P. Ruszel Andrzej Stępniewski Dariusz Gałkowski Jacek Bogucki Przemysław Kołodziej Jolanta Szymańska Bartosz J. Płachno Tomasz Zubilewicz Marcin Feldo Janusz Kocki Anna Bogucka-Kocka Identification of Transcriptomic Differences between Lower Extremities Arterial Disease, Abdominal Aortic Aneurysm and Chronic Venous Disease in Peripheral Blood Mononuclear Cells Specimens International Journal of Molecular Sciences lower extremities arterial disease chronic venous disease abdominal aortic aneurysm gene expression next generation sequencing biomarker |
| title | Identification of Transcriptomic Differences between Lower Extremities Arterial Disease, Abdominal Aortic Aneurysm and Chronic Venous Disease in Peripheral Blood Mononuclear Cells Specimens |
| title_full | Identification of Transcriptomic Differences between Lower Extremities Arterial Disease, Abdominal Aortic Aneurysm and Chronic Venous Disease in Peripheral Blood Mononuclear Cells Specimens |
| title_fullStr | Identification of Transcriptomic Differences between Lower Extremities Arterial Disease, Abdominal Aortic Aneurysm and Chronic Venous Disease in Peripheral Blood Mononuclear Cells Specimens |
| title_full_unstemmed | Identification of Transcriptomic Differences between Lower Extremities Arterial Disease, Abdominal Aortic Aneurysm and Chronic Venous Disease in Peripheral Blood Mononuclear Cells Specimens |
| title_short | Identification of Transcriptomic Differences between Lower Extremities Arterial Disease, Abdominal Aortic Aneurysm and Chronic Venous Disease in Peripheral Blood Mononuclear Cells Specimens |
| title_sort | identification of transcriptomic differences between lower extremities arterial disease abdominal aortic aneurysm and chronic venous disease in peripheral blood mononuclear cells specimens |
| topic | lower extremities arterial disease chronic venous disease abdominal aortic aneurysm gene expression next generation sequencing biomarker |
| url | https://www.mdpi.com/1422-0067/22/6/3200 |
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