A new approach on essential oil production of Origanum onites L.: Microbial fertilization and microwave extraction
In many studies plant and microbe interaction has been observed due to its stimulating effect on plant oil content. Bacillus spp. isolates used as microbial fertilizer/biostimulant have shown a higher potential for enhancing plant production and quality. However, there is still a need for investigat...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023074194 |
| _version_ | 1797669724763455488 |
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| author | Nurgül Kıtır Şen Ahmet Duran |
| author_facet | Nurgül Kıtır Şen Ahmet Duran |
| author_sort | Nurgül Kıtır Şen |
| collection | DOAJ |
| description | In many studies plant and microbe interaction has been observed due to its stimulating effect on plant oil content. Bacillus spp. isolates used as microbial fertilizer/biostimulant have shown a higher potential for enhancing plant production and quality. However, there is still a need for investigating the essential oil content of plants, particularly for the pharmaceutical industry, to increase the targeted essential oil quantity and quality. Thyme is a crucial plant in medicinal aromatic herbal production and export, and its economic value is steadily increasing due to its high essential oil content. This study explores a novel approach to increase essential oil content through microbial fertilization (biostimulation) and microwave extraction methods. The microwave extraction method offers comparable yields to other methods, requiring less time, energy, and only a small amount of water to hydrate the dried plants. Microbial fertilizer formulations were applied to İzmir thyme (Origanum onites L.) to assess their effects on yield and quality. Three distinct microbial fertilizer formulations (N, P, NP) containing bacterial isolates of Bacillus subtilis and Bacillus megaterium, as well as a consortium of these two bacteria, were employed in this study. Among the applications on İzmir thyme, the NP formulation (a combination of two bacteria at a dosage of 6 L/ha−1) exhibited the highest efficacy in terms of yield and quality criteria. Moreover, the essential oil ratios (1–2%) obtained from the same fertilizer application, using microwave extraction to enhance quality and accelerate the extraction process, yielded higher than other methods.This study provides preliminary data and a new approach to regulate and enhance the targeted yield and essential oil content of thyme plants by utilizing microbial fertilizer applications and the microwave extraction method. |
| first_indexed | 2024-03-11T20:48:42Z |
| format | Article |
| id | doaj.art-b0c332dc8945485f9d5b3f99f18735d2 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-11T20:48:42Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-b0c332dc8945485f9d5b3f99f18735d22023-10-01T06:02:52ZengElsevierHeliyon2405-84402023-09-0199e20211A new approach on essential oil production of Origanum onites L.: Microbial fertilization and microwave extractionNurgül Kıtır Şen0Ahmet Duran1Gebze Techmical University, Institute of Earth and Marine Sciences, 41400, Gebze, Kocaeli, Turkey; Corresponding author.Konya Food and Agriculture University, Institute of Sciences, Department of Biotechnology, 42080, Konya, TurkeyIn many studies plant and microbe interaction has been observed due to its stimulating effect on plant oil content. Bacillus spp. isolates used as microbial fertilizer/biostimulant have shown a higher potential for enhancing plant production and quality. However, there is still a need for investigating the essential oil content of plants, particularly for the pharmaceutical industry, to increase the targeted essential oil quantity and quality. Thyme is a crucial plant in medicinal aromatic herbal production and export, and its economic value is steadily increasing due to its high essential oil content. This study explores a novel approach to increase essential oil content through microbial fertilization (biostimulation) and microwave extraction methods. The microwave extraction method offers comparable yields to other methods, requiring less time, energy, and only a small amount of water to hydrate the dried plants. Microbial fertilizer formulations were applied to İzmir thyme (Origanum onites L.) to assess their effects on yield and quality. Three distinct microbial fertilizer formulations (N, P, NP) containing bacterial isolates of Bacillus subtilis and Bacillus megaterium, as well as a consortium of these two bacteria, were employed in this study. Among the applications on İzmir thyme, the NP formulation (a combination of two bacteria at a dosage of 6 L/ha−1) exhibited the highest efficacy in terms of yield and quality criteria. Moreover, the essential oil ratios (1–2%) obtained from the same fertilizer application, using microwave extraction to enhance quality and accelerate the extraction process, yielded higher than other methods.This study provides preliminary data and a new approach to regulate and enhance the targeted yield and essential oil content of thyme plants by utilizing microbial fertilizer applications and the microwave extraction method.http://www.sciencedirect.com/science/article/pii/S2405844023074194Origanum onites LPGPBMicrobial fertilizer (biostimulant)YieldQualityEssential oil |
| spellingShingle | Nurgül Kıtır Şen Ahmet Duran A new approach on essential oil production of Origanum onites L.: Microbial fertilization and microwave extraction Heliyon Origanum onites L PGPB Microbial fertilizer (biostimulant) Yield Quality Essential oil |
| title | A new approach on essential oil production of Origanum onites L.: Microbial fertilization and microwave extraction |
| title_full | A new approach on essential oil production of Origanum onites L.: Microbial fertilization and microwave extraction |
| title_fullStr | A new approach on essential oil production of Origanum onites L.: Microbial fertilization and microwave extraction |
| title_full_unstemmed | A new approach on essential oil production of Origanum onites L.: Microbial fertilization and microwave extraction |
| title_short | A new approach on essential oil production of Origanum onites L.: Microbial fertilization and microwave extraction |
| title_sort | new approach on essential oil production of origanum onites l microbial fertilization and microwave extraction |
| topic | Origanum onites L PGPB Microbial fertilizer (biostimulant) Yield Quality Essential oil |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023074194 |
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