APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES

APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES

Abstract. Present work describes a multiplex “sandvich” approach to enzyme-linked, solid-phase immunosorbent assay designed in microarray formate, aiming for quantitative evaluation of individual profile of cytokine expression. Its high specificity, throughput and sensitivity (30 to 20,000 pg/mL) ar...

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Bibliographic Details
Main Authors: M. A. Plotnikova, S. A. Klotchenko, V. V. Egorov, K. P. Nikulenkov, A. V. Vasin, O. I. Kisselev
Format: Article
Language:Russian
Published: St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists 2014-07-01
Series:Медицинская иммунология
Subjects:
cytokines
antibodies
microarray
immunological analysis
Online Access:https://www.mimmun.ru/mimmun/article/view/378
Description
Summary:Abstract. Present work describes a multiplex “sandvich” approach to enzyme-linked, solid-phase immunosorbent assay designed in microarray formate, aiming for quantitative evaluation of individual profile of cytokine expression. Its high specificity, throughput and sensitivity (30 to 20,000 pg/mL) are demonstrated with human IL-2, IL-4, IL-10, and TNF assays. This diagnostic technique may be further used both for research applications and in clinical trials.
ISSN:1563-0625
2313-741X

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