Gas-Mediated Intestinal Microbiome Regulation Prompts the Methanol Extract of <i>Schizonepetae Spica</i> to Relieve Colitis
Intestinal dysbiosis plays an important role in the pathogenesis of colitis (UC). <i>Schizonepetae Herba</i> can achieve anti-inflammatory effects as a medicine and food homologous vegetable. Luteolin, eriodictyol, fisetin, and kaempferol are the main anti-inflammatory active compounds o...
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MDPI AG
2023-01-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/3/519 |
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| author | Xuewei Ye Yingxin Cen Kefei Wu Langyu Xu Jiahui Ni Wenxin Zheng Wei Liu |
| author_facet | Xuewei Ye Yingxin Cen Kefei Wu Langyu Xu Jiahui Ni Wenxin Zheng Wei Liu |
| author_sort | Xuewei Ye |
| collection | DOAJ |
| description | Intestinal dysbiosis plays an important role in the pathogenesis of colitis (UC). <i>Schizonepetae Herba</i> can achieve anti-inflammatory effects as a medicine and food homologous vegetable. Luteolin, eriodictyol, fisetin, and kaempferol are the main anti-inflammatory active compounds obtained through mass spectrometry from the methanol extract of <i>Schizonepetae Spica</i> (JJSM). JJSM intervention resulted in attenuated weight loss, high disease-activity-index score, colon length shortening and colonic pathological damage in DSS-induced colitis mice. Interestingly, hydrogen sulfide (H<sub>2</sub>S) was inhibited remarkably, which is helpful to elucidate the relationship between active substance and intestinal flora. Furthermore, JJSM administration improved intestinal flora with down-regulating the abundance of harmful bacteria such as <i>Clostridiales</i> and <i>Desulfovibrio</i> and up-regulating the abundance of beneficial bacteria such as <i>Muribaculaceae</i> and <i>Ligolactobacillus</i> and enhanced the production of SCFAs. It is worth noticing that <i>Desulfovibrio</i> is related to the production of intestinal gas H<sub>2</sub>S. The elevated levels of <i>Desulfovibrio</i> and H<sub>2</sub>S will hasten the onset of colitis, which is a crucial risk factor for colitis. The results displayed that JJSM could considerably ameliorate colitis by rebuilding H<sub>2</sub>S-related intestinal flora, which provides a new therapeutic strategy for <i>Schizonepetae Spica</i> to be utilized as a functional food and considered as an emerging candidate for intestinal inflammation. |
| first_indexed | 2024-03-11T09:31:39Z |
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| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T09:31:39Z |
| publishDate | 2023-01-01 |
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| series | Nutrients |
| spelling | doaj.art-b0c4eb2c97da4ca4a7ef7b9c8486ae912023-11-16T17:38:05ZengMDPI AGNutrients2072-66432023-01-0115351910.3390/nu15030519Gas-Mediated Intestinal Microbiome Regulation Prompts the Methanol Extract of <i>Schizonepetae Spica</i> to Relieve ColitisXuewei Ye0Yingxin Cen1Kefei Wu2Langyu Xu3Jiahui Ni4Wenxin Zheng5Wei Liu6Department of Basic Medical Sciences, Shulan International Medical College, Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Zhejiang Shuren University, Hangzhou 310015, ChinaDepartment of Basic Medical Sciences, Shulan International Medical College, Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Zhejiang Shuren University, Hangzhou 310015, ChinaDepartment of Basic Medical Sciences, Shulan International Medical College, Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Zhejiang Shuren University, Hangzhou 310015, ChinaDepartment of Basic Medical Sciences, Shulan International Medical College, Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Zhejiang Shuren University, Hangzhou 310015, ChinaDepartment of Basic Medical Sciences, Shulan International Medical College, Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Zhejiang Shuren University, Hangzhou 310015, ChinaDepartment of Basic Medical Sciences, Shulan International Medical College, Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Zhejiang Shuren University, Hangzhou 310015, ChinaInstitute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaIntestinal dysbiosis plays an important role in the pathogenesis of colitis (UC). <i>Schizonepetae Herba</i> can achieve anti-inflammatory effects as a medicine and food homologous vegetable. Luteolin, eriodictyol, fisetin, and kaempferol are the main anti-inflammatory active compounds obtained through mass spectrometry from the methanol extract of <i>Schizonepetae Spica</i> (JJSM). JJSM intervention resulted in attenuated weight loss, high disease-activity-index score, colon length shortening and colonic pathological damage in DSS-induced colitis mice. Interestingly, hydrogen sulfide (H<sub>2</sub>S) was inhibited remarkably, which is helpful to elucidate the relationship between active substance and intestinal flora. Furthermore, JJSM administration improved intestinal flora with down-regulating the abundance of harmful bacteria such as <i>Clostridiales</i> and <i>Desulfovibrio</i> and up-regulating the abundance of beneficial bacteria such as <i>Muribaculaceae</i> and <i>Ligolactobacillus</i> and enhanced the production of SCFAs. It is worth noticing that <i>Desulfovibrio</i> is related to the production of intestinal gas H<sub>2</sub>S. The elevated levels of <i>Desulfovibrio</i> and H<sub>2</sub>S will hasten the onset of colitis, which is a crucial risk factor for colitis. The results displayed that JJSM could considerably ameliorate colitis by rebuilding H<sub>2</sub>S-related intestinal flora, which provides a new therapeutic strategy for <i>Schizonepetae Spica</i> to be utilized as a functional food and considered as an emerging candidate for intestinal inflammation.https://www.mdpi.com/2072-6643/15/3/519<i>Schizonepetae Spica</i>ulcerative colitisintestinal flora<i>Desulfovibrio</i>H<sub>2</sub>S |
| spellingShingle | Xuewei Ye Yingxin Cen Kefei Wu Langyu Xu Jiahui Ni Wenxin Zheng Wei Liu Gas-Mediated Intestinal Microbiome Regulation Prompts the Methanol Extract of <i>Schizonepetae Spica</i> to Relieve Colitis Nutrients <i>Schizonepetae Spica</i> ulcerative colitis intestinal flora <i>Desulfovibrio</i> H<sub>2</sub>S |
| title | Gas-Mediated Intestinal Microbiome Regulation Prompts the Methanol Extract of <i>Schizonepetae Spica</i> to Relieve Colitis |
| title_full | Gas-Mediated Intestinal Microbiome Regulation Prompts the Methanol Extract of <i>Schizonepetae Spica</i> to Relieve Colitis |
| title_fullStr | Gas-Mediated Intestinal Microbiome Regulation Prompts the Methanol Extract of <i>Schizonepetae Spica</i> to Relieve Colitis |
| title_full_unstemmed | Gas-Mediated Intestinal Microbiome Regulation Prompts the Methanol Extract of <i>Schizonepetae Spica</i> to Relieve Colitis |
| title_short | Gas-Mediated Intestinal Microbiome Regulation Prompts the Methanol Extract of <i>Schizonepetae Spica</i> to Relieve Colitis |
| title_sort | gas mediated intestinal microbiome regulation prompts the methanol extract of i schizonepetae spica i to relieve colitis |
| topic | <i>Schizonepetae Spica</i> ulcerative colitis intestinal flora <i>Desulfovibrio</i> H<sub>2</sub>S |
| url | https://www.mdpi.com/2072-6643/15/3/519 |
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