L-arginine and tetrahydrobiopterin modulate endothelin-1A receptor activity in isolated rat aorta
Endothelin-1 (ET-1), is a potent endogenous vasoconstrictor secreted by endothelial cells. It acts as the natural counterpart of the vasodilator effect of nitric oxide (NO). ET-1 exert it is the vasoconstrictor activity through two types of receptors, ET-1A and ET-1B receptors that are located on va...
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| Format: | Article |
| Language: | English |
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Salahaddin University-Erbil
2019-04-01
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| Series: | Zanco Journal of Pure and Applied Sciences |
| Subjects: | |
| Online Access: | https://zancojournals.su.edu.krd/index.php/JPAS/article/view/2670 |
| _version_ | 1819140158611521536 |
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| author | Zhikal Omar Khidhr Ismail Mustafa Maulood, professor |
| author_facet | Zhikal Omar Khidhr Ismail Mustafa Maulood, professor |
| author_sort | Zhikal Omar Khidhr |
| collection | DOAJ |
| description | Endothelin-1 (ET-1), is a potent endogenous vasoconstrictor secreted by endothelial cells. It acts as the natural counterpart of the vasodilator effect of nitric oxide (NO). ET-1 exert it is the vasoconstrictor activity through two types of receptors, ET-1A and ET-1B receptors that are located on vascular smooth muscle cells (VSMCs). The present study was designed to evaluate the effect of L-arginine (LA), tetrahydrobiopterin (BH4), and their combination on ET-1A receptor activity in rat aortic rings. The study involved pre-incubation of rat aortic rings with LA, BH4, and their combination. Then, the vascular response to a cumulative dose of the ET-1A receptor antagonist (BQ-123) and in the second set of experiment cumulative doses of Acetylcholine (ACh) were applied to each group. BQ-123 potency increased after LA pre-incubation, but LA and BH4 in combination significantly potentiated BQ-123 potency and maximum response. In the second set of experiment, ACh potency does not change, while ACh efficacy markedly increased during pre-incubation of LA, BH4, and their combination. In conclusion, LA and BH4 may offer some pharmacological tools to modulate the ET-1A receptor activity and treat cardiovascular disease beyond pulmonary arterial hypertension. |
| first_indexed | 2024-12-22T11:34:07Z |
| format | Article |
| id | doaj.art-b0c604182ce547eeb1110b49bb9a729f |
| institution | Directory Open Access Journal |
| issn | 2218-0230 2412-3986 |
| language | English |
| last_indexed | 2024-12-22T11:34:07Z |
| publishDate | 2019-04-01 |
| publisher | Salahaddin University-Erbil |
| record_format | Article |
| series | Zanco Journal of Pure and Applied Sciences |
| spelling | doaj.art-b0c604182ce547eeb1110b49bb9a729f2022-12-21T18:27:29ZengSalahaddin University-ErbilZanco Journal of Pure and Applied Sciences2218-02302412-39862019-04-0131210110810.21271/ZJPAS.31.2.13L-arginine and tetrahydrobiopterin modulate endothelin-1A receptor activity in isolated rat aortaZhikal Omar Khidhr0Ismail Mustafa Maulood, professor1Department of Biology, College of Science, Salahaddin University, Erbil, Iraq Department of Biology, College of Science, Salahaddin University, Erbil, IraqEndothelin-1 (ET-1), is a potent endogenous vasoconstrictor secreted by endothelial cells. It acts as the natural counterpart of the vasodilator effect of nitric oxide (NO). ET-1 exert it is the vasoconstrictor activity through two types of receptors, ET-1A and ET-1B receptors that are located on vascular smooth muscle cells (VSMCs). The present study was designed to evaluate the effect of L-arginine (LA), tetrahydrobiopterin (BH4), and their combination on ET-1A receptor activity in rat aortic rings. The study involved pre-incubation of rat aortic rings with LA, BH4, and their combination. Then, the vascular response to a cumulative dose of the ET-1A receptor antagonist (BQ-123) and in the second set of experiment cumulative doses of Acetylcholine (ACh) were applied to each group. BQ-123 potency increased after LA pre-incubation, but LA and BH4 in combination significantly potentiated BQ-123 potency and maximum response. In the second set of experiment, ACh potency does not change, while ACh efficacy markedly increased during pre-incubation of LA, BH4, and their combination. In conclusion, LA and BH4 may offer some pharmacological tools to modulate the ET-1A receptor activity and treat cardiovascular disease beyond pulmonary arterial hypertension.https://zancojournals.su.edu.krd/index.php/JPAS/article/view/2670Acetylcholine; BQ-123; Endothelin-1; L-arginine; Rat aorta; Tetrahydrobiopterin. |
| spellingShingle | Zhikal Omar Khidhr Ismail Mustafa Maulood, professor L-arginine and tetrahydrobiopterin modulate endothelin-1A receptor activity in isolated rat aorta Zanco Journal of Pure and Applied Sciences Acetylcholine; BQ-123; Endothelin-1; L-arginine; Rat aorta; Tetrahydrobiopterin. |
| title | L-arginine and tetrahydrobiopterin modulate endothelin-1A receptor activity in isolated rat aorta |
| title_full | L-arginine and tetrahydrobiopterin modulate endothelin-1A receptor activity in isolated rat aorta |
| title_fullStr | L-arginine and tetrahydrobiopterin modulate endothelin-1A receptor activity in isolated rat aorta |
| title_full_unstemmed | L-arginine and tetrahydrobiopterin modulate endothelin-1A receptor activity in isolated rat aorta |
| title_short | L-arginine and tetrahydrobiopterin modulate endothelin-1A receptor activity in isolated rat aorta |
| title_sort | l arginine and tetrahydrobiopterin modulate endothelin 1a receptor activity in isolated rat aorta |
| topic | Acetylcholine; BQ-123; Endothelin-1; L-arginine; Rat aorta; Tetrahydrobiopterin. |
| url | https://zancojournals.su.edu.krd/index.php/JPAS/article/view/2670 |
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