Industrial and Ruminant Trans-Fatty Acids-Enriched Diets Differentially Modulate the Microbiome and Fecal Metabolites in C57BL/6 Mice
Industrially originated trans-fatty acids (I-<i>t</i>FAs), such as elaidic acid (EA), and ruminant trans-fatty acids (R-<i>t</i>FAs), such as <i>trans</i>-palmitoleic acid (TPA), may have opposite effects on metabolic health. The objective was to compare the effec...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/6/1433 |
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| author | Farzad Mohammadi Miranda Green Emma Tolsdorf Karine Greffard Mickael Leclercq Jean-François Bilodeau Arnaud Droit Jane Foster Nicolas Bertrand Iwona Rudkowska |
| author_facet | Farzad Mohammadi Miranda Green Emma Tolsdorf Karine Greffard Mickael Leclercq Jean-François Bilodeau Arnaud Droit Jane Foster Nicolas Bertrand Iwona Rudkowska |
| author_sort | Farzad Mohammadi |
| collection | DOAJ |
| description | Industrially originated trans-fatty acids (I-<i>t</i>FAs), such as elaidic acid (EA), and ruminant trans-fatty acids (R-<i>t</i>FAs), such as <i>trans</i>-palmitoleic acid (TPA), may have opposite effects on metabolic health. The objective was to compare the effects of consuming 2–3% I-<i>t</i>FA or R-<i>t</i>FA on the gut microbiome and fecal metabolite profile in mice after 7 and 28 days. Forty C57BL/6 mice were assigned to one of the four prepared formulations: lecithin nanovesicles, lecithin nanovesicles with EA or TPA, or water. Fecal samples and animals’ weights were collected on days 0, 7, and 28. Fecal samples were used to determine gut microbiome profiles by 16S rRNA sequencing and metabolite concentrations by GC/MS. At 28 days, TPA intake decreased the abundance of <i>Staphylococcus</i> sp55 but increased <i>Staphylococcus</i> sp119. EA intake also increased the abundance of <i>Staphylococcus</i> sp119 but decreased <i>Ruminococcaceae</i> UCG-014, <i>Lachnospiraceae</i>, and <i>Clostridium sensu stricto</i> 1 at 28 days. Fecal short-chain fatty acids were increased after TPA while decreased after EA after 7 and 28 days. This study shows that TPA and EA modify the abundance of specific microbial taxa and fecal metabolite profiles in distinct ways. |
| first_indexed | 2024-03-11T06:04:29Z |
| format | Article |
| id | doaj.art-b0c6cc44b46d4ae29ea1f898dc19735b |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T06:04:29Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-b0c6cc44b46d4ae29ea1f898dc19735b2023-11-17T13:05:44ZengMDPI AGNutrients2072-66432023-03-01156143310.3390/nu15061433Industrial and Ruminant Trans-Fatty Acids-Enriched Diets Differentially Modulate the Microbiome and Fecal Metabolites in C57BL/6 MiceFarzad Mohammadi0Miranda Green1Emma Tolsdorf2Karine Greffard3Mickael Leclercq4Jean-François Bilodeau5Arnaud Droit6Jane Foster7Nicolas Bertrand8Iwona Rudkowska9Endocrinology and Nephrology Unit, CHU de Québec-Université Laval Research Center, Québec, QC G1V 4G2, CanadaDepartment of Psychiatry and Behavioral Neurosciences, McMaster University, Hamilton, ON L8S 4L8, CanadaDepartment of Psychiatry and Behavioral Neurosciences, McMaster University, Hamilton, ON L8S 4L8, CanadaEndocrinology and Nephrology Unit, CHU de Québec-Université Laval Research Center, Québec, QC G1V 4G2, CanadaEndocrinology and Nephrology Unit, CHU de Québec-Université Laval Research Center, Québec, QC G1V 4G2, CanadaEndocrinology and Nephrology Unit, CHU de Québec-Université Laval Research Center, Québec, QC G1V 4G2, CanadaEndocrinology and Nephrology Unit, CHU de Québec-Université Laval Research Center, Québec, QC G1V 4G2, CanadaDepartment of Psychiatry and Behavioral Neurosciences, McMaster University, Hamilton, ON L8S 4L8, CanadaEndocrinology and Nephrology Unit, CHU de Québec-Université Laval Research Center, Québec, QC G1V 4G2, CanadaEndocrinology and Nephrology Unit, CHU de Québec-Université Laval Research Center, Québec, QC G1V 4G2, CanadaIndustrially originated trans-fatty acids (I-<i>t</i>FAs), such as elaidic acid (EA), and ruminant trans-fatty acids (R-<i>t</i>FAs), such as <i>trans</i>-palmitoleic acid (TPA), may have opposite effects on metabolic health. The objective was to compare the effects of consuming 2–3% I-<i>t</i>FA or R-<i>t</i>FA on the gut microbiome and fecal metabolite profile in mice after 7 and 28 days. Forty C57BL/6 mice were assigned to one of the four prepared formulations: lecithin nanovesicles, lecithin nanovesicles with EA or TPA, or water. Fecal samples and animals’ weights were collected on days 0, 7, and 28. Fecal samples were used to determine gut microbiome profiles by 16S rRNA sequencing and metabolite concentrations by GC/MS. At 28 days, TPA intake decreased the abundance of <i>Staphylococcus</i> sp55 but increased <i>Staphylococcus</i> sp119. EA intake also increased the abundance of <i>Staphylococcus</i> sp119 but decreased <i>Ruminococcaceae</i> UCG-014, <i>Lachnospiraceae</i>, and <i>Clostridium sensu stricto</i> 1 at 28 days. Fecal short-chain fatty acids were increased after TPA while decreased after EA after 7 and 28 days. This study shows that TPA and EA modify the abundance of specific microbial taxa and fecal metabolite profiles in distinct ways.https://www.mdpi.com/2072-6643/15/6/1433industrial trans-fatty acid (I-<i>t</i>FA)metabolitesmicrobiomesruminant trans-fatty acid (R-<i>t</i>FA)short-chain fatty acids (SCFAs) |
| spellingShingle | Farzad Mohammadi Miranda Green Emma Tolsdorf Karine Greffard Mickael Leclercq Jean-François Bilodeau Arnaud Droit Jane Foster Nicolas Bertrand Iwona Rudkowska Industrial and Ruminant Trans-Fatty Acids-Enriched Diets Differentially Modulate the Microbiome and Fecal Metabolites in C57BL/6 Mice Nutrients industrial trans-fatty acid (I-<i>t</i>FA) metabolites microbiomes ruminant trans-fatty acid (R-<i>t</i>FA) short-chain fatty acids (SCFAs) |
| title | Industrial and Ruminant Trans-Fatty Acids-Enriched Diets Differentially Modulate the Microbiome and Fecal Metabolites in C57BL/6 Mice |
| title_full | Industrial and Ruminant Trans-Fatty Acids-Enriched Diets Differentially Modulate the Microbiome and Fecal Metabolites in C57BL/6 Mice |
| title_fullStr | Industrial and Ruminant Trans-Fatty Acids-Enriched Diets Differentially Modulate the Microbiome and Fecal Metabolites in C57BL/6 Mice |
| title_full_unstemmed | Industrial and Ruminant Trans-Fatty Acids-Enriched Diets Differentially Modulate the Microbiome and Fecal Metabolites in C57BL/6 Mice |
| title_short | Industrial and Ruminant Trans-Fatty Acids-Enriched Diets Differentially Modulate the Microbiome and Fecal Metabolites in C57BL/6 Mice |
| title_sort | industrial and ruminant trans fatty acids enriched diets differentially modulate the microbiome and fecal metabolites in c57bl 6 mice |
| topic | industrial trans-fatty acid (I-<i>t</i>FA) metabolites microbiomes ruminant trans-fatty acid (R-<i>t</i>FA) short-chain fatty acids (SCFAs) |
| url | https://www.mdpi.com/2072-6643/15/6/1433 |
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