MOLECULAR DETECTION AND DIFFERENTIATION OF MYCOPLASMA SPECIES IN SWINE LUNG TISSUES
Mycoplasma species are ubiquitous and cause both primary and secondary infections in swine. Most of these Mycoplasma species are fastidious and require stringent growth requirements. Molecular detection and differentiation of these Mycoplasma species directly from field samples have greater diagno...
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| Format: | Article |
| Language: | English |
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West Bengal Veterinary Alumni Association
2023-06-01
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| Series: | Exploratory Animal and Medical Research |
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| Online Access: | https://www.animalmedicalresearch.org/Vol.13_Issue-1_June_2023/MOLECULAR%20DETECTION%20AND.pdf |
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| author | Thahira parveen S Parthiban S Raja P Parthiban M Dhinakar Raj G |
| author_facet | Thahira parveen S Parthiban S Raja P Parthiban M Dhinakar Raj G |
| author_sort | Thahira parveen S |
| collection | DOAJ |
| description | Mycoplasma species are ubiquitous and cause both primary and secondary infections in swine. Most of
these Mycoplasma species are fastidious and require stringent growth requirements. Molecular detection and differentiation
of these Mycoplasma species directly from field samples have greater diagnostic value. A total of 57 porcine tissue samples
collected from different regions of Tamil Nadu suspected of respiratory tract infections were screened with a PCR assay
targeting the 16SrRNA gene of Mycoplasma and a multiplex PCR to identify and differentiate M. hyopneumonia, M.
focculare, and M. hyorhinis infections. The PCR-based screening with 16SrRNA genus-specific primer confirmed Mycoplasma
infection in four (7%) of the 57 samples screened with a specific amplicon of 717 bp. Multiplex PCR analysis of all 57 samples
showed a similar positivity rate of 7% (n=4), demonstrating the presence of three 1129 bp and one 754 bp amplicons specific
to M. hyorhinis and M. flocculare genome respectively in pneumonic lesions of swine from Tamil Nadu. The specificity of the
M. hyorhinis genome was further confirmed by sequencing and BLAST analysis. A BLAST homology search revealed 99%
sequence identity with European Mycoplasma isolates. Phylogenetic analysis in MEGA X, through the Maximum Likelihood
tree (MLT) method revealed specific clustering along with M. hyorhinis species. The assembled partial contig sequence M.
hyorhinis was deposited in the GenBank database under the accession number MW822751. |
| first_indexed | 2024-03-12T22:52:18Z |
| format | Article |
| id | doaj.art-b0c8016e78c849c38651d36edf83e9fd |
| institution | Directory Open Access Journal |
| issn | 2277-470X 2319-247X |
| language | English |
| last_indexed | 2024-03-12T22:52:18Z |
| publishDate | 2023-06-01 |
| publisher | West Bengal Veterinary Alumni Association |
| record_format | Article |
| series | Exploratory Animal and Medical Research |
| spelling | doaj.art-b0c8016e78c849c38651d36edf83e9fd2023-07-20T07:55:58ZengWest Bengal Veterinary Alumni AssociationExploratory Animal and Medical Research2277-470X2319-247X2023-06-011319810410.52635/eamr/13.1.98-104MOLECULAR DETECTION AND DIFFERENTIATION OF MYCOPLASMA SPECIES IN SWINE LUNG TISSUESThahira parveen S0Parthiban S1Raja P2Parthiban M3Dhinakar Raj G4Jeppiaar Engineering College, Chennai, Tamilnadu, India.Department of Animal Biotechnology, Madras Veterinary College, Chennai-600007, Tamil Nadu Veterinary and Animal Sciences University, Tamilnadu, India.Department of Animal Biotechnology, Madras Veterinary College, Chennai-600007, Tamil Nadu Veterinary and Animal Sciences University, Tamilnadu, India.Department of Animal Biotechnology, Madras Veterinary College, Chennai-600007, Tamil Nadu Veterinary and Animal Sciences University, Tamilnadu, India.Department of Animal Biotechnology, Madras Veterinary College, Chennai-600007, Tamil Nadu Veterinary and Animal Sciences University, Tamilnadu, India.Mycoplasma species are ubiquitous and cause both primary and secondary infections in swine. Most of these Mycoplasma species are fastidious and require stringent growth requirements. Molecular detection and differentiation of these Mycoplasma species directly from field samples have greater diagnostic value. A total of 57 porcine tissue samples collected from different regions of Tamil Nadu suspected of respiratory tract infections were screened with a PCR assay targeting the 16SrRNA gene of Mycoplasma and a multiplex PCR to identify and differentiate M. hyopneumonia, M. focculare, and M. hyorhinis infections. The PCR-based screening with 16SrRNA genus-specific primer confirmed Mycoplasma infection in four (7%) of the 57 samples screened with a specific amplicon of 717 bp. Multiplex PCR analysis of all 57 samples showed a similar positivity rate of 7% (n=4), demonstrating the presence of three 1129 bp and one 754 bp amplicons specific to M. hyorhinis and M. flocculare genome respectively in pneumonic lesions of swine from Tamil Nadu. The specificity of the M. hyorhinis genome was further confirmed by sequencing and BLAST analysis. A BLAST homology search revealed 99% sequence identity with European Mycoplasma isolates. Phylogenetic analysis in MEGA X, through the Maximum Likelihood tree (MLT) method revealed specific clustering along with M. hyorhinis species. The assembled partial contig sequence M. hyorhinis was deposited in the GenBank database under the accession number MW822751.https://www.animalmedicalresearch.org/Vol.13_Issue-1_June_2023/MOLECULAR%20DETECTION%20AND.pdfmycoplasma sppswinedetectionmultiplex-pcrphylogenetic analysis |
| spellingShingle | Thahira parveen S Parthiban S Raja P Parthiban M Dhinakar Raj G MOLECULAR DETECTION AND DIFFERENTIATION OF MYCOPLASMA SPECIES IN SWINE LUNG TISSUES Exploratory Animal and Medical Research mycoplasma spp swine detection multiplex-pcr phylogenetic analysis |
| title | MOLECULAR DETECTION AND DIFFERENTIATION OF MYCOPLASMA SPECIES IN SWINE LUNG TISSUES |
| title_full | MOLECULAR DETECTION AND DIFFERENTIATION OF MYCOPLASMA SPECIES IN SWINE LUNG TISSUES |
| title_fullStr | MOLECULAR DETECTION AND DIFFERENTIATION OF MYCOPLASMA SPECIES IN SWINE LUNG TISSUES |
| title_full_unstemmed | MOLECULAR DETECTION AND DIFFERENTIATION OF MYCOPLASMA SPECIES IN SWINE LUNG TISSUES |
| title_short | MOLECULAR DETECTION AND DIFFERENTIATION OF MYCOPLASMA SPECIES IN SWINE LUNG TISSUES |
| title_sort | molecular detection and differentiation of mycoplasma species in swine lung tissues |
| topic | mycoplasma spp swine detection multiplex-pcr phylogenetic analysis |
| url | https://www.animalmedicalresearch.org/Vol.13_Issue-1_June_2023/MOLECULAR%20DETECTION%20AND.pdf |
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