A Detailed Study of Antimicrobial Sensitivity Pattern of Penton Valentine Leucocidin Gene Positive and Negative Staphylococcus aureus from Pus Samples
Introduction: Staphylococcus aureus harboring Panton Valentine Leucocidin gene are emerging and spreading worldwide. PVL gene was first identified by Noel Panton and Francis Valentine in 1932 who IC Pakistan only limited data is available regarding the effect of PVL gene on sensitivity pattern of St...
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| Format: | Article |
| Language: | English |
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Shaikh Zayed Medical Complex, Lahore
2020-12-01
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| Series: | Proceedings |
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| Online Access: | https://proceedings-szmc.org.pk/index.php/szmc/article/view/19 |
| _version_ | 1811181915744501760 |
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| author | Mateen Izhar Asma Akram Anwaar Basheer Mariya Ali Asma Yaqoob Asim Saifullah |
| author_facet | Mateen Izhar Asma Akram Anwaar Basheer Mariya Ali Asma Yaqoob Asim Saifullah |
| author_sort | Mateen Izhar |
| collection | DOAJ |
| description | Introduction: Staphylococcus aureus harboring Panton Valentine Leucocidin gene are emerging and spreading worldwide. PVL gene was first identified by Noel Panton and Francis Valentine in 1932 who IC Pakistan only limited data is available regarding the effect of PVL gene on sensitivity pattern of Staphylococcus aureus. Therefore, this study was conducted to understand the antimicrobial sensitivity pattern of both PVL positive and negative Staphylococcus aureus isolates.
Aims & Objectives: This study was conducted to understand the antimicrobial sensitivity pattern of both PVL positive and PVL negative Staphylococcus aureus isolated from pus samples received from various indoor and outdoor departments of a tertiary care hospital of Lahore.
Place and duration of study: Microbiology and Molecular Biology Laboratory Shaikh Zayed Hospital Lahore. Duration of study is one year after the approval of research topic.
Material & Methods: A total of 384 Staphylococcus aureus isolates from skin and soft tissue infections were identified and selected. Their antimicrobial sensitivity testing was done by Kirby disc diffusion method using Muller Hinton agar.
Results: Frequencies of PVL gene in MRSA and MSSA were 51% and 44% respectively. Frequency of PVL gene was also found to be high in Ciprofloxacin sensitive, Gentamicin sensitive, Erythromycin resistant and fusidic acid resistant isolates.
Conclusion: Almost half of Staphylococcus aureus isolates were found PVL positive. They were mostly multidrug resistant. The PVL positive Staphylococcus aureus isolates showed high resistance against antibiotics than PVL negative isolates |
| first_indexed | 2024-04-11T09:24:59Z |
| format | Article |
| id | doaj.art-b0c82d36882a457a9336258c54c98b6b |
| institution | Directory Open Access Journal |
| issn | 1815-4905 2518-203X |
| language | English |
| last_indexed | 2024-04-11T09:24:59Z |
| publishDate | 2020-12-01 |
| publisher | Shaikh Zayed Medical Complex, Lahore |
| record_format | Article |
| series | Proceedings |
| spelling | doaj.art-b0c82d36882a457a9336258c54c98b6b2022-12-22T04:32:03ZengShaikh Zayed Medical Complex, LahoreProceedings1815-49052518-203X2020-12-01343242810.47489/p000s343z7571-5mc19A Detailed Study of Antimicrobial Sensitivity Pattern of Penton Valentine Leucocidin Gene Positive and Negative Staphylococcus aureus from Pus SamplesMateen Izhar0Asma Akram1Anwaar Basheer2Mariya Ali3Asma Yaqoob4Asim Saifullah5Department of Microbiology, Shaikh Zayed Medical Complex, Lahore 1 Department of Pathology, Continental Medical College, Lahore Department of Microbiology, Shaikh Zayed Medical Complex, LahoreDepartment of Pathology, Continental Medical College, LahoreDepartment of Pathology, Fatima Memorial Medical and Dental College, Lahore Department of Pediatric Orthopedic Surgery, Children Hospital, Lahore Introduction: Staphylococcus aureus harboring Panton Valentine Leucocidin gene are emerging and spreading worldwide. PVL gene was first identified by Noel Panton and Francis Valentine in 1932 who IC Pakistan only limited data is available regarding the effect of PVL gene on sensitivity pattern of Staphylococcus aureus. Therefore, this study was conducted to understand the antimicrobial sensitivity pattern of both PVL positive and negative Staphylococcus aureus isolates. Aims & Objectives: This study was conducted to understand the antimicrobial sensitivity pattern of both PVL positive and PVL negative Staphylococcus aureus isolated from pus samples received from various indoor and outdoor departments of a tertiary care hospital of Lahore. Place and duration of study: Microbiology and Molecular Biology Laboratory Shaikh Zayed Hospital Lahore. Duration of study is one year after the approval of research topic. Material & Methods: A total of 384 Staphylococcus aureus isolates from skin and soft tissue infections were identified and selected. Their antimicrobial sensitivity testing was done by Kirby disc diffusion method using Muller Hinton agar. Results: Frequencies of PVL gene in MRSA and MSSA were 51% and 44% respectively. Frequency of PVL gene was also found to be high in Ciprofloxacin sensitive, Gentamicin sensitive, Erythromycin resistant and fusidic acid resistant isolates. Conclusion: Almost half of Staphylococcus aureus isolates were found PVL positive. They were mostly multidrug resistant. The PVL positive Staphylococcus aureus isolates showed high resistance against antibiotics than PVL negative isolateshttps://proceedings-szmc.org.pk/index.php/szmc/article/view/19pvl (panton valentine leucocidin), mrsa (methicillin resistant staphylococcus aureus) |
| spellingShingle | Mateen Izhar Asma Akram Anwaar Basheer Mariya Ali Asma Yaqoob Asim Saifullah A Detailed Study of Antimicrobial Sensitivity Pattern of Penton Valentine Leucocidin Gene Positive and Negative Staphylococcus aureus from Pus Samples Proceedings pvl (panton valentine leucocidin), mrsa (methicillin resistant staphylococcus aureus) |
| title | A Detailed Study of Antimicrobial Sensitivity Pattern of Penton Valentine Leucocidin Gene Positive and Negative Staphylococcus aureus from Pus Samples |
| title_full | A Detailed Study of Antimicrobial Sensitivity Pattern of Penton Valentine Leucocidin Gene Positive and Negative Staphylococcus aureus from Pus Samples |
| title_fullStr | A Detailed Study of Antimicrobial Sensitivity Pattern of Penton Valentine Leucocidin Gene Positive and Negative Staphylococcus aureus from Pus Samples |
| title_full_unstemmed | A Detailed Study of Antimicrobial Sensitivity Pattern of Penton Valentine Leucocidin Gene Positive and Negative Staphylococcus aureus from Pus Samples |
| title_short | A Detailed Study of Antimicrobial Sensitivity Pattern of Penton Valentine Leucocidin Gene Positive and Negative Staphylococcus aureus from Pus Samples |
| title_sort | detailed study of antimicrobial sensitivity pattern of penton valentine leucocidin gene positive and negative staphylococcus aureus from pus samples |
| topic | pvl (panton valentine leucocidin), mrsa (methicillin resistant staphylococcus aureus) |
| url | https://proceedings-szmc.org.pk/index.php/szmc/article/view/19 |
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