Construction of chicken infectious anemia virus infectious clone and study on its pathogenicity
Chicken infectious anemia virus (CIAV) can be transmitted through contaminated live poultry vaccine. However, the pathogenicity of contaminated CIAV strains is rarely reported. Previously, the chickens showed the typical symptoms of anemia after using the attenuated live fowl pox virus (FPV) vaccine...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1016784/full |
| _version_ | 1798001687599775744 |
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| author | Jinjin Wang Yan Li Yawen Zhang Longfei Chen Lichun Fang Shuang Chang Yixin Wang Peng Zhao |
| author_facet | Jinjin Wang Yan Li Yawen Zhang Longfei Chen Lichun Fang Shuang Chang Yixin Wang Peng Zhao |
| author_sort | Jinjin Wang |
| collection | DOAJ |
| description | Chicken infectious anemia virus (CIAV) can be transmitted through contaminated live poultry vaccine. However, the pathogenicity of contaminated CIAV strains is rarely reported. Previously, the chickens showed the typical symptoms of anemia after using the attenuated live fowl pox virus (FPV) vaccine. Therefore, exogenous CIAV contamination was suspected. We detected anti-CIAV antibodies in SPF chicks vaccinated with the FPV vaccine. CIAV contamination was confirmed in the FPV vaccine, and the CIAV strain was named JS2020-FPV. This study aims to rescue JS2020-FPV by reverse genetic assays and investigate its pathogenicity. Firstly, double-copies infectious clone of JS2020-FPV was constructed. For the pathogenicity study, infectious clone of JS2020-FPV was used to inoculate 1-day-old SPF chicks. The typical symptoms of anemia were observed in the JS2020-PFV group 14 days post inoculation. The hematocrit and body weight of chicks in the JS2020-PFV group were significantly lower than those in the mock group. Notably, the thymus development index and antibody levels of NDV were lower in chicks in the JS2020-PFV group than those in the mock group. Different degrees of apoptosis of MSB1 and DF-1 were observed after inoculated with the JS2020-FPV VP3 recombinant fusion protein expressed by E. coli system, indicating that VP3 induced apoptosis in the transformed cells. Overall, the pathogenicity of the CIAV detected in the contaminated vaccine was confirmed by inoculating SPF chicks with the double-copies infectious DNA clone in this study. Our findings indicate that the dangers of vaccine contamination cannot be ignored. |
| first_indexed | 2024-04-11T11:40:19Z |
| format | Article |
| id | doaj.art-b0c9cdda09984d71ac43d29cddfe2cdc |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T11:40:19Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-b0c9cdda09984d71ac43d29cddfe2cdc2022-12-22T04:25:51ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.10167841016784Construction of chicken infectious anemia virus infectious clone and study on its pathogenicityJinjin Wang0Yan Li1Yawen Zhang2Longfei Chen3Lichun Fang4Shuang Chang5Yixin Wang6Peng Zhao7College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an, ChinaCollege of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an, ChinaCollege of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an, ChinaCollege of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an, ChinaShandong Academy of Agricultural Sciences, Jinan, ChinaCollege of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an, ChinaCollege of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an, ChinaCollege of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an, ChinaChicken infectious anemia virus (CIAV) can be transmitted through contaminated live poultry vaccine. However, the pathogenicity of contaminated CIAV strains is rarely reported. Previously, the chickens showed the typical symptoms of anemia after using the attenuated live fowl pox virus (FPV) vaccine. Therefore, exogenous CIAV contamination was suspected. We detected anti-CIAV antibodies in SPF chicks vaccinated with the FPV vaccine. CIAV contamination was confirmed in the FPV vaccine, and the CIAV strain was named JS2020-FPV. This study aims to rescue JS2020-FPV by reverse genetic assays and investigate its pathogenicity. Firstly, double-copies infectious clone of JS2020-FPV was constructed. For the pathogenicity study, infectious clone of JS2020-FPV was used to inoculate 1-day-old SPF chicks. The typical symptoms of anemia were observed in the JS2020-PFV group 14 days post inoculation. The hematocrit and body weight of chicks in the JS2020-PFV group were significantly lower than those in the mock group. Notably, the thymus development index and antibody levels of NDV were lower in chicks in the JS2020-PFV group than those in the mock group. Different degrees of apoptosis of MSB1 and DF-1 were observed after inoculated with the JS2020-FPV VP3 recombinant fusion protein expressed by E. coli system, indicating that VP3 induced apoptosis in the transformed cells. Overall, the pathogenicity of the CIAV detected in the contaminated vaccine was confirmed by inoculating SPF chicks with the double-copies infectious DNA clone in this study. Our findings indicate that the dangers of vaccine contamination cannot be ignored.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1016784/fullchicken infectious anemia viruspathogenicityVP3 fusion proteinfowl pox virusinfectious clone |
| spellingShingle | Jinjin Wang Yan Li Yawen Zhang Longfei Chen Lichun Fang Shuang Chang Yixin Wang Peng Zhao Construction of chicken infectious anemia virus infectious clone and study on its pathogenicity Frontiers in Microbiology chicken infectious anemia virus pathogenicity VP3 fusion protein fowl pox virus infectious clone |
| title | Construction of chicken infectious anemia virus infectious clone and study on its pathogenicity |
| title_full | Construction of chicken infectious anemia virus infectious clone and study on its pathogenicity |
| title_fullStr | Construction of chicken infectious anemia virus infectious clone and study on its pathogenicity |
| title_full_unstemmed | Construction of chicken infectious anemia virus infectious clone and study on its pathogenicity |
| title_short | Construction of chicken infectious anemia virus infectious clone and study on its pathogenicity |
| title_sort | construction of chicken infectious anemia virus infectious clone and study on its pathogenicity |
| topic | chicken infectious anemia virus pathogenicity VP3 fusion protein fowl pox virus infectious clone |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1016784/full |
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