Comparative proteomics of three Chinese potato cultivars to improve understanding of potato molecular response to late blight disease
Abstract Background Late blight disease (LBD) caused by the pathogen Phytophthora infestans (PI), is the most devastating disease limiting potato (Solanum tuberosum) production globally. Currently, this disease pathogen is re-emerging and appearing in new areas at a very high intensity. A better und...
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| Format: | Article |
| Language: | English |
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BMC
2020-12-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-020-07286-3 |
| _version_ | 1818594788397547520 |
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| author | Chunfang Xiao Mengling Huang Jianhua Gao Zhen Wang Denghong Zhang Yuanxue Zhang Lei Yan Xiao Yu Bo Li Yanfen Shen |
| author_facet | Chunfang Xiao Mengling Huang Jianhua Gao Zhen Wang Denghong Zhang Yuanxue Zhang Lei Yan Xiao Yu Bo Li Yanfen Shen |
| author_sort | Chunfang Xiao |
| collection | DOAJ |
| description | Abstract Background Late blight disease (LBD) caused by the pathogen Phytophthora infestans (PI), is the most devastating disease limiting potato (Solanum tuberosum) production globally. Currently, this disease pathogen is re-emerging and appearing in new areas at a very high intensity. A better understanding of the natural defense mechanisms against PI in different potato cultivars especially at the protein level is still lacking. Therefore, to elucidate potato proteome response to PI, we investigated changes in the proteome and leaf morphology of three potato cultivars, namely; Favorita (FA), Mira (MA), and E-malingshu N0.14 (E14) infected with PI by using the iTRAQ-based quantitative proteomics analysis. Results A total of 3306 proteins were found in the three potato genotypes, and 2044 proteins were quantified. Cluster analysis revealed MA and E14 clustered together separately from FA. The protein profile and related functions revealed that the cultivars shared a typical hypersensitive response to PI, including induction of elicitors, oxidative burst, and suppression of photosynthesis in the potato leaves. Meanwhile, MA and E14 deployed additional specific response mechanism different from FA, involving high induction of protease inhibitors, serine/threonine kinases, terpenoid, hormone signaling, and transport, which contributed to MA tolerance of LBD. Furthermore, inductions of pathogenesis-related proteins, LRR receptor-like kinases, mitogen-activated protein kinase, WRKY transcription factors, jasmonic acid, and phenolic compounds mediate E14 resistance against LBD. These proteins were confirmed at the transcription level by a quantitative polymerase chain reaction and at the translation level by western-blot. Conclusions We found several proteins that were differentially abundant among the cultivars, that includes common and cultivar specific proteins which highlighted similarities and significant differences between FA, MA, and E14 in terms of their defense response to PI. Here the specific accumulation of mitogen-activated protein kinase, Serine/threonine kinases, WRKY transcription played a positive role in E14 immunity against PI. The candidate proteins identified reported in this study will form the basis of future studies and may improve our understanding of the molecular mechanisms of late blight disease resistance in potato. |
| first_indexed | 2024-12-16T11:05:41Z |
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| id | doaj.art-b0cfd73f811e47308e5b746b93ba0467 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-16T11:05:41Z |
| publishDate | 2020-12-01 |
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| series | BMC Genomics |
| spelling | doaj.art-b0cfd73f811e47308e5b746b93ba04672022-12-21T22:33:52ZengBMCBMC Genomics1471-21642020-12-0121112110.1186/s12864-020-07286-3Comparative proteomics of three Chinese potato cultivars to improve understanding of potato molecular response to late blight diseaseChunfang Xiao0Mengling Huang1Jianhua Gao2Zhen Wang3Denghong Zhang4Yuanxue Zhang5Lei Yan6Xiao Yu7Bo Li8Yanfen Shen9State Key Laboratory of Agricultural Microbiology and Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural UniversityState Key Laboratory of Agricultural Microbiology and Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural UniversitySouthern Potato Research Center of ChinaSouthern Potato Research Center of ChinaSouthern Potato Research Center of ChinaSouthern Potato Research Center of ChinaSouthern Potato Research Center of ChinaState Key Laboratory of Agricultural Microbiology and Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural UniversityState Key Laboratory of Agricultural Microbiology and Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural UniversitySouthern Potato Research Center of ChinaAbstract Background Late blight disease (LBD) caused by the pathogen Phytophthora infestans (PI), is the most devastating disease limiting potato (Solanum tuberosum) production globally. Currently, this disease pathogen is re-emerging and appearing in new areas at a very high intensity. A better understanding of the natural defense mechanisms against PI in different potato cultivars especially at the protein level is still lacking. Therefore, to elucidate potato proteome response to PI, we investigated changes in the proteome and leaf morphology of three potato cultivars, namely; Favorita (FA), Mira (MA), and E-malingshu N0.14 (E14) infected with PI by using the iTRAQ-based quantitative proteomics analysis. Results A total of 3306 proteins were found in the three potato genotypes, and 2044 proteins were quantified. Cluster analysis revealed MA and E14 clustered together separately from FA. The protein profile and related functions revealed that the cultivars shared a typical hypersensitive response to PI, including induction of elicitors, oxidative burst, and suppression of photosynthesis in the potato leaves. Meanwhile, MA and E14 deployed additional specific response mechanism different from FA, involving high induction of protease inhibitors, serine/threonine kinases, terpenoid, hormone signaling, and transport, which contributed to MA tolerance of LBD. Furthermore, inductions of pathogenesis-related proteins, LRR receptor-like kinases, mitogen-activated protein kinase, WRKY transcription factors, jasmonic acid, and phenolic compounds mediate E14 resistance against LBD. These proteins were confirmed at the transcription level by a quantitative polymerase chain reaction and at the translation level by western-blot. Conclusions We found several proteins that were differentially abundant among the cultivars, that includes common and cultivar specific proteins which highlighted similarities and significant differences between FA, MA, and E14 in terms of their defense response to PI. Here the specific accumulation of mitogen-activated protein kinase, Serine/threonine kinases, WRKY transcription played a positive role in E14 immunity against PI. The candidate proteins identified reported in this study will form the basis of future studies and may improve our understanding of the molecular mechanisms of late blight disease resistance in potato.https://doi.org/10.1186/s12864-020-07286-3Comparative proteomicsPotato cultivarsPhytophthora infestansLate blight diseaseHypersensitive responseSusceptible |
| spellingShingle | Chunfang Xiao Mengling Huang Jianhua Gao Zhen Wang Denghong Zhang Yuanxue Zhang Lei Yan Xiao Yu Bo Li Yanfen Shen Comparative proteomics of three Chinese potato cultivars to improve understanding of potato molecular response to late blight disease BMC Genomics Comparative proteomics Potato cultivars Phytophthora infestans Late blight disease Hypersensitive response Susceptible |
| title | Comparative proteomics of three Chinese potato cultivars to improve understanding of potato molecular response to late blight disease |
| title_full | Comparative proteomics of three Chinese potato cultivars to improve understanding of potato molecular response to late blight disease |
| title_fullStr | Comparative proteomics of three Chinese potato cultivars to improve understanding of potato molecular response to late blight disease |
| title_full_unstemmed | Comparative proteomics of three Chinese potato cultivars to improve understanding of potato molecular response to late blight disease |
| title_short | Comparative proteomics of three Chinese potato cultivars to improve understanding of potato molecular response to late blight disease |
| title_sort | comparative proteomics of three chinese potato cultivars to improve understanding of potato molecular response to late blight disease |
| topic | Comparative proteomics Potato cultivars Phytophthora infestans Late blight disease Hypersensitive response Susceptible |
| url | https://doi.org/10.1186/s12864-020-07286-3 |
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