Effect of Bioaugmentation with Anaerobic Fungi Isolated from Ruminants on the Hydrolysis of Corn Silage and <i>Phragmites australis</i>
Anaerobic fungi produce extracellular hydrolytic enzymes that facilitate degradation of cellulose and hemicellulose in ruminants. The purpose of this work was to study the impact of three different anaerobic fungal species (<i>Anaeromyces mucronatus</i> YE505, <i>Neocallimastix fro...
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MDPI AG
2021-09-01
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| author | Bhargavi Ravi Valentine Nkongndem Nkemka Xiying Hao Jay Yanke Tim A. McAllister Hung Lee Chitraichamy Veluchamy Brandon H. Gilroyed |
| author_facet | Bhargavi Ravi Valentine Nkongndem Nkemka Xiying Hao Jay Yanke Tim A. McAllister Hung Lee Chitraichamy Veluchamy Brandon H. Gilroyed |
| author_sort | Bhargavi Ravi |
| collection | DOAJ |
| description | Anaerobic fungi produce extracellular hydrolytic enzymes that facilitate degradation of cellulose and hemicellulose in ruminants. The purpose of this work was to study the impact of three different anaerobic fungal species (<i>Anaeromyces mucronatus</i> YE505, <i>Neocallimastix frontalis</i> 27, and <i>Piromyces rhizinflatus</i> YM600) on hydrolysis of two different lignocellulosic substrates, corn (<i>Zea mays</i> L.) silage and reed (<i>Phragmites australis</i> (Cav.) Trin. ex Steud.). Biomass from each plant species was incubated anaerobically for 11 days either in the presence of live fungal inoculum or with heat-inactivated (control) inoculum. Headspace gas composition, dry matter loss, soluble chemical oxygen demand, concentration of volatile fatty acids, and chemical composition were measured before and after hydrolysis. While some microbial activity was observed, inoculation with anaerobic fungi did not result in any significant difference in the degradation of either type of plant biomass tested, likely due to low fungal activity or survival under the experimental conditions tested. While the premise of utilizing the unique biological activities of anaerobic fungi for biotechnology applications remains promising, further research on optimizing culturing and process conditions is necessary. |
| first_indexed | 2024-03-10T07:06:00Z |
| format | Article |
| id | doaj.art-b0d216a910c9460ca144cf3127108e58 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T07:06:00Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-b0d216a910c9460ca144cf3127108e582023-11-22T15:48:05ZengMDPI AGApplied Sciences2076-34172021-09-011119912310.3390/app11199123Effect of Bioaugmentation with Anaerobic Fungi Isolated from Ruminants on the Hydrolysis of Corn Silage and <i>Phragmites australis</i>Bhargavi Ravi0Valentine Nkongndem Nkemka1Xiying Hao2Jay Yanke3Tim A. McAllister4Hung Lee5Chitraichamy Veluchamy6Brandon H. Gilroyed7School of Environmental Sciences, University of Guelph Ridgetown Campus, Ridgetown, ON N0P 2C0, CanadaAgriculture and Agri-Food Canada, Lethbridge Research Station, Lethbridge, AB T1J 4P4, CanadaAgriculture and Agri-Food Canada, Lethbridge Research Station, Lethbridge, AB T1J 4P4, CanadaAgriculture and Agri-Food Canada, Lethbridge Research Station, Lethbridge, AB T1J 4P4, CanadaAgriculture and Agri-Food Canada, Lethbridge Research Station, Lethbridge, AB T1J 4P4, CanadaSchool of Environmental Sciences, University of Guelph, Guelph, ON N1G 2W1, CanadaSchool of Environmental Sciences, University of Guelph Ridgetown Campus, Ridgetown, ON N0P 2C0, CanadaSchool of Environmental Sciences, University of Guelph Ridgetown Campus, Ridgetown, ON N0P 2C0, CanadaAnaerobic fungi produce extracellular hydrolytic enzymes that facilitate degradation of cellulose and hemicellulose in ruminants. The purpose of this work was to study the impact of three different anaerobic fungal species (<i>Anaeromyces mucronatus</i> YE505, <i>Neocallimastix frontalis</i> 27, and <i>Piromyces rhizinflatus</i> YM600) on hydrolysis of two different lignocellulosic substrates, corn (<i>Zea mays</i> L.) silage and reed (<i>Phragmites australis</i> (Cav.) Trin. ex Steud.). Biomass from each plant species was incubated anaerobically for 11 days either in the presence of live fungal inoculum or with heat-inactivated (control) inoculum. Headspace gas composition, dry matter loss, soluble chemical oxygen demand, concentration of volatile fatty acids, and chemical composition were measured before and after hydrolysis. While some microbial activity was observed, inoculation with anaerobic fungi did not result in any significant difference in the degradation of either type of plant biomass tested, likely due to low fungal activity or survival under the experimental conditions tested. While the premise of utilizing the unique biological activities of anaerobic fungi for biotechnology applications remains promising, further research on optimizing culturing and process conditions is necessary.https://www.mdpi.com/2076-3417/11/19/9123<i>Anaeromyces mucronatus</i>lignocellulose<i>Neocallimastix frontalis</i><i>Piromyces rhizinflatus</i>pretreatmenthydrogen |
| spellingShingle | Bhargavi Ravi Valentine Nkongndem Nkemka Xiying Hao Jay Yanke Tim A. McAllister Hung Lee Chitraichamy Veluchamy Brandon H. Gilroyed Effect of Bioaugmentation with Anaerobic Fungi Isolated from Ruminants on the Hydrolysis of Corn Silage and <i>Phragmites australis</i> Applied Sciences <i>Anaeromyces mucronatus</i> lignocellulose <i>Neocallimastix frontalis</i> <i>Piromyces rhizinflatus</i> pretreatment hydrogen |
| title | Effect of Bioaugmentation with Anaerobic Fungi Isolated from Ruminants on the Hydrolysis of Corn Silage and <i>Phragmites australis</i> |
| title_full | Effect of Bioaugmentation with Anaerobic Fungi Isolated from Ruminants on the Hydrolysis of Corn Silage and <i>Phragmites australis</i> |
| title_fullStr | Effect of Bioaugmentation with Anaerobic Fungi Isolated from Ruminants on the Hydrolysis of Corn Silage and <i>Phragmites australis</i> |
| title_full_unstemmed | Effect of Bioaugmentation with Anaerobic Fungi Isolated from Ruminants on the Hydrolysis of Corn Silage and <i>Phragmites australis</i> |
| title_short | Effect of Bioaugmentation with Anaerobic Fungi Isolated from Ruminants on the Hydrolysis of Corn Silage and <i>Phragmites australis</i> |
| title_sort | effect of bioaugmentation with anaerobic fungi isolated from ruminants on the hydrolysis of corn silage and i phragmites australis i |
| topic | <i>Anaeromyces mucronatus</i> lignocellulose <i>Neocallimastix frontalis</i> <i>Piromyces rhizinflatus</i> pretreatment hydrogen |
| url | https://www.mdpi.com/2076-3417/11/19/9123 |
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