Combined transcriptomic and physiological metabolomic analyses elucidate key biological pathways in the response of two sorghum genotypes to salinity stress
Sorghum is an important food crop with high salt tolerance. Therefore, studying the salt tolerance mechanism of sorghum has great significance for understanding the salt tolerance mechanism of C4 plants. In this study, two sorghum species, LRNK1 (salt-tolerant (ST)) and LR2381 (salt-sensitive (SS)),...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.880373/full |
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| author | Fei Zhang Feng Lu Yanqiu Wang Zhipeng Zhang Jiaxu Wang Kuangye Zhang Han Wu Jianqiu Zou Youhou Duan Fulai Ke Kai Zhu |
| author_facet | Fei Zhang Feng Lu Yanqiu Wang Zhipeng Zhang Jiaxu Wang Kuangye Zhang Han Wu Jianqiu Zou Youhou Duan Fulai Ke Kai Zhu |
| author_sort | Fei Zhang |
| collection | DOAJ |
| description | Sorghum is an important food crop with high salt tolerance. Therefore, studying the salt tolerance mechanism of sorghum has great significance for understanding the salt tolerance mechanism of C4 plants. In this study, two sorghum species, LRNK1 (salt-tolerant (ST)) and LR2381 (salt-sensitive (SS)), were treated with 180 mM NaCl salt solution, and their physiological indicators were measured. Transcriptomic and metabolomic analyses were performed by Illumina sequencing and liquid chromatography-mass spectrometry (LC-MS) technology, respectively. The results demonstrated that the plant height, leaf area, and chlorophyll contents in LRNK1 were significantly higher than in LR2381. Functional analysis of differently expressed genes (DEGs) demonstrated that plant hormone signal transduction (GO:0015473), carbohydrate catabolic processes (GO:0016052), and photosynthesis (GO:0015979) were the main pathways to respond to salt stress in sorghum. The genes of the two varieties showed different expression patterns under salt stress conditions. The metabolomic data revealed different profiles of salicylic acid and betaine between LRNK1 and LR2381, which mediated the salt tolerance of sorghum. In conclusion, LRNK1 sorghum responds to salt stress via a variety of biological processes, including energy reserve, the accumulation of salicylic acid and betaine, and improving the activity of salt stress-related pathways. These discoveries provide new insights into the salt tolerance mechanism of sorghum and will contribute to sorghum breeding. |
| first_indexed | 2024-04-13T19:08:28Z |
| format | Article |
| id | doaj.art-b0d5031336e54a8d9730cba3be71589e |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T19:08:28Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-b0d5031336e54a8d9730cba3be71589e2022-12-22T02:33:55ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.880373880373Combined transcriptomic and physiological metabolomic analyses elucidate key biological pathways in the response of two sorghum genotypes to salinity stressFei ZhangFeng LuYanqiu WangZhipeng ZhangJiaxu WangKuangye ZhangHan WuJianqiu ZouYouhou DuanFulai KeKai ZhuSorghum is an important food crop with high salt tolerance. Therefore, studying the salt tolerance mechanism of sorghum has great significance for understanding the salt tolerance mechanism of C4 plants. In this study, two sorghum species, LRNK1 (salt-tolerant (ST)) and LR2381 (salt-sensitive (SS)), were treated with 180 mM NaCl salt solution, and their physiological indicators were measured. Transcriptomic and metabolomic analyses were performed by Illumina sequencing and liquid chromatography-mass spectrometry (LC-MS) technology, respectively. The results demonstrated that the plant height, leaf area, and chlorophyll contents in LRNK1 were significantly higher than in LR2381. Functional analysis of differently expressed genes (DEGs) demonstrated that plant hormone signal transduction (GO:0015473), carbohydrate catabolic processes (GO:0016052), and photosynthesis (GO:0015979) were the main pathways to respond to salt stress in sorghum. The genes of the two varieties showed different expression patterns under salt stress conditions. The metabolomic data revealed different profiles of salicylic acid and betaine between LRNK1 and LR2381, which mediated the salt tolerance of sorghum. In conclusion, LRNK1 sorghum responds to salt stress via a variety of biological processes, including energy reserve, the accumulation of salicylic acid and betaine, and improving the activity of salt stress-related pathways. These discoveries provide new insights into the salt tolerance mechanism of sorghum and will contribute to sorghum breeding.https://www.frontiersin.org/articles/10.3389/fpls.2022.880373/fullsorghumresistancesalt stresstranscriptomicmetabolomicsalicylic acid |
| spellingShingle | Fei Zhang Feng Lu Yanqiu Wang Zhipeng Zhang Jiaxu Wang Kuangye Zhang Han Wu Jianqiu Zou Youhou Duan Fulai Ke Kai Zhu Combined transcriptomic and physiological metabolomic analyses elucidate key biological pathways in the response of two sorghum genotypes to salinity stress Frontiers in Plant Science sorghum resistance salt stress transcriptomic metabolomic salicylic acid |
| title | Combined transcriptomic and physiological metabolomic analyses elucidate key biological pathways in the response of two sorghum genotypes to salinity stress |
| title_full | Combined transcriptomic and physiological metabolomic analyses elucidate key biological pathways in the response of two sorghum genotypes to salinity stress |
| title_fullStr | Combined transcriptomic and physiological metabolomic analyses elucidate key biological pathways in the response of two sorghum genotypes to salinity stress |
| title_full_unstemmed | Combined transcriptomic and physiological metabolomic analyses elucidate key biological pathways in the response of two sorghum genotypes to salinity stress |
| title_short | Combined transcriptomic and physiological metabolomic analyses elucidate key biological pathways in the response of two sorghum genotypes to salinity stress |
| title_sort | combined transcriptomic and physiological metabolomic analyses elucidate key biological pathways in the response of two sorghum genotypes to salinity stress |
| topic | sorghum resistance salt stress transcriptomic metabolomic salicylic acid |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.880373/full |
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