The Transcriptomic Landscape of Pediatric Astrocytoma
Central nervous system tumors are the most common solid neoplasia during childhood and represent one of the leading causes of cancer-related mortality. Tumors arising from astrocytic cells (astrocytomas) are the most frequently diagnosed, and according to their histological and pathological characte...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/20/12696 |
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| author | Abrahan Hernández-Hernández Tayde López-Santaella Aranxa Torres-Caballero Amarantha Serrato Ulises Torres-Flores Diego Montesinos-Valencia Fernando Chico-Ponce de León Vicente González-Carranza Samuel Torres-García Rosa Rebollar-Vega Inti Alberto De la Rosa-Velázquez Rosario Ortiz Monserrat Pérez-Ramírez Normand García-Hernández Antonio García-Méndez Francisco Arenas-Huertero |
| author_facet | Abrahan Hernández-Hernández Tayde López-Santaella Aranxa Torres-Caballero Amarantha Serrato Ulises Torres-Flores Diego Montesinos-Valencia Fernando Chico-Ponce de León Vicente González-Carranza Samuel Torres-García Rosa Rebollar-Vega Inti Alberto De la Rosa-Velázquez Rosario Ortiz Monserrat Pérez-Ramírez Normand García-Hernández Antonio García-Méndez Francisco Arenas-Huertero |
| author_sort | Abrahan Hernández-Hernández |
| collection | DOAJ |
| description | Central nervous system tumors are the most common solid neoplasia during childhood and represent one of the leading causes of cancer-related mortality. Tumors arising from astrocytic cells (astrocytomas) are the most frequently diagnosed, and according to their histological and pathological characteristics, they are classified into four categories. However, an additional layer of molecular classification considering the DNA sequence of the tumorigenesis-associated genes <i>IDH1/2</i> and <i>H3F3A</i> has recently been incorporated into the classification guidelines. Although mutations in <i>H3F3A</i> are found exclusively in a subtype of grade IV pediatric astrocytoma, mutations in <i>IDH1/2</i> genes are very rare in children under 14 years of age. The transcriptomic profiles of astrocytoma in adults and children have been extensively studied. However, there is scarce information on these profiles in pediatric populations considering the status of tumorigenesis-associated genes. Therefore, here we report the transcriptomic landscape of the four grades of pediatric astrocytoma by RNA sequencing. We found several well-documented biological functions associated with the misregulated genes in the four grades of astrocytoma, as well as additional biological pathways. Among the four grades of astrocytoma, we found shared misregulated genes that could have implications in tumorigenesis. Finally, we identified a transcriptional signature for almost all grades of astrocytoma that could be used as a transcription-based identification method. |
| first_indexed | 2024-03-09T20:05:05Z |
| format | Article |
| id | doaj.art-b0da84b077044c6d884f69d2ce27c9d4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T20:05:05Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b0da84b077044c6d884f69d2ce27c9d42023-11-24T00:35:33ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123201269610.3390/ijms232012696The Transcriptomic Landscape of Pediatric AstrocytomaAbrahan Hernández-Hernández0Tayde López-Santaella1Aranxa Torres-Caballero2Amarantha Serrato3Ulises Torres-Flores4Diego Montesinos-Valencia5Fernando Chico-Ponce de León6Vicente González-Carranza7Samuel Torres-García8Rosa Rebollar-Vega9Inti Alberto De la Rosa-Velázquez10Rosario Ortiz11Monserrat Pérez-Ramírez12Normand García-Hernández13Antonio García-Méndez14Francisco Arenas-Huertero15Biología de Células Individuales (BIOCELIN), Hospital Infantil de México Federico Gómez, Ciudad de México 06720, MexicoBiología de Células Individuales (BIOCELIN), Hospital Infantil de México Federico Gómez, Ciudad de México 06720, MexicoBiología de Células Individuales (BIOCELIN), Hospital Infantil de México Federico Gómez, Ciudad de México 06720, MexicoBiología de Células Individuales (BIOCELIN), Hospital Infantil de México Federico Gómez, Ciudad de México 06720, MexicoBiología de Células Individuales (BIOCELIN), Hospital Infantil de México Federico Gómez, Ciudad de México 06720, MexicoBiología de Células Individuales (BIOCELIN), Hospital Infantil de México Federico Gómez, Ciudad de México 06720, MexicoDepartamento de Neurología, Hospital Infantil de México Federico Gómez, Ciudad de México 06720, MexicoDepartamento de Neurología, Hospital Infantil de México Federico Gómez, Ciudad de México 06720, MexicoDepartamento de Neurología, Hospital Infantil de México Federico Gómez, Ciudad de México 06720, MexicoLaboratorio de Genómica, Red de Apoyo a la Investigación, Universidad Nacional Autónoma de México, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México City 04510, MexicoLaboratorio de Genómica, Red de Apoyo a la Investigación, Universidad Nacional Autónoma de México, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México City 04510, MexicoLaboratorio de Microscopía Electrónica, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoUnidad de Investigación Médica en Genética Humana, Centro Médico Nacional Siglo XXI, IMSS, Hospital de Pediatría, Ciudad de México 06720, MexicoUnidad de Investigación Médica en Genética Humana, Centro Médico Nacional Siglo XXI, IMSS, Hospital de Pediatría, Ciudad de México 06720, MexicoCentro Médico Nacional, La Raza, IMSS, Hospital General Gaudencio González Garza, Ciudad de México 04510, MexicoLaboratorio de Investigación en Patología Experimental, Hospital Infantil de México Federico Gómez, Ciudad de México 06720, MexicoCentral nervous system tumors are the most common solid neoplasia during childhood and represent one of the leading causes of cancer-related mortality. Tumors arising from astrocytic cells (astrocytomas) are the most frequently diagnosed, and according to their histological and pathological characteristics, they are classified into four categories. However, an additional layer of molecular classification considering the DNA sequence of the tumorigenesis-associated genes <i>IDH1/2</i> and <i>H3F3A</i> has recently been incorporated into the classification guidelines. Although mutations in <i>H3F3A</i> are found exclusively in a subtype of grade IV pediatric astrocytoma, mutations in <i>IDH1/2</i> genes are very rare in children under 14 years of age. The transcriptomic profiles of astrocytoma in adults and children have been extensively studied. However, there is scarce information on these profiles in pediatric populations considering the status of tumorigenesis-associated genes. Therefore, here we report the transcriptomic landscape of the four grades of pediatric astrocytoma by RNA sequencing. We found several well-documented biological functions associated with the misregulated genes in the four grades of astrocytoma, as well as additional biological pathways. Among the four grades of astrocytoma, we found shared misregulated genes that could have implications in tumorigenesis. Finally, we identified a transcriptional signature for almost all grades of astrocytoma that could be used as a transcription-based identification method.https://www.mdpi.com/1422-0067/23/20/12696pediatric astrocytomatranscriptomeRNA-seq |
| spellingShingle | Abrahan Hernández-Hernández Tayde López-Santaella Aranxa Torres-Caballero Amarantha Serrato Ulises Torres-Flores Diego Montesinos-Valencia Fernando Chico-Ponce de León Vicente González-Carranza Samuel Torres-García Rosa Rebollar-Vega Inti Alberto De la Rosa-Velázquez Rosario Ortiz Monserrat Pérez-Ramírez Normand García-Hernández Antonio García-Méndez Francisco Arenas-Huertero The Transcriptomic Landscape of Pediatric Astrocytoma International Journal of Molecular Sciences pediatric astrocytoma transcriptome RNA-seq |
| title | The Transcriptomic Landscape of Pediatric Astrocytoma |
| title_full | The Transcriptomic Landscape of Pediatric Astrocytoma |
| title_fullStr | The Transcriptomic Landscape of Pediatric Astrocytoma |
| title_full_unstemmed | The Transcriptomic Landscape of Pediatric Astrocytoma |
| title_short | The Transcriptomic Landscape of Pediatric Astrocytoma |
| title_sort | transcriptomic landscape of pediatric astrocytoma |
| topic | pediatric astrocytoma transcriptome RNA-seq |
| url | https://www.mdpi.com/1422-0067/23/20/12696 |
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