The Crystal Structure of <i>Bacillus cereus</i> HblL<sub>1</sub>
The Hbl toxin is a three-component haemolytic complex produced by <i>Bacillus cereus sensu lato</i> strains and implicated as a cause of diarrhoea in <i>B. cereus</i> food poisoning. While the structure of the HblB component of this toxin is known, the structures of the other...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/13/4/253 |
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| author | Harley L. Worthy Lainey J. Williamson Husam Sabah Auhim Stephen H. Leppla Inka Sastalla D. Dafydd Jones Pierre J. Rizkallah Colin Berry |
| author_facet | Harley L. Worthy Lainey J. Williamson Husam Sabah Auhim Stephen H. Leppla Inka Sastalla D. Dafydd Jones Pierre J. Rizkallah Colin Berry |
| author_sort | Harley L. Worthy |
| collection | DOAJ |
| description | The Hbl toxin is a three-component haemolytic complex produced by <i>Bacillus cereus sensu lato</i> strains and implicated as a cause of diarrhoea in <i>B. cereus</i> food poisoning. While the structure of the HblB component of this toxin is known, the structures of the other components are unresolved. Here, we describe the expression of the recombinant HblL<sub>1</sub> component and the elucidation of its structure to 1.36 Å. Like HblB, it is a member of the alpha-helical pore-forming toxin family. In comparison to other members of this group, it has an extended hydrophobic beta tongue region that may be involved in pore formation. Molecular docking was used to predict possible interactions between HblL<sub>1</sub> and HblB, and suggests a head to tail dimer might form, burying the HblL<sub>1</sub> beta tongue region. |
| first_indexed | 2024-03-10T12:44:49Z |
| format | Article |
| id | doaj.art-b0dc8e12acd44a729c284ab42d0a7198 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T12:44:49Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-b0dc8e12acd44a729c284ab42d0a71982023-11-21T13:36:40ZengMDPI AGToxins2072-66512021-03-0113425310.3390/toxins13040253The Crystal Structure of <i>Bacillus cereus</i> HblL<sub>1</sub>Harley L. Worthy0Lainey J. Williamson1Husam Sabah Auhim2Stephen H. Leppla3Inka Sastalla4D. Dafydd Jones5Pierre J. Rizkallah6Colin Berry7School of Biosciences, Cardiff University, Park Place, Cardiff CF10 3AX, UKSchool of Biosciences, Cardiff University, Park Place, Cardiff CF10 3AX, UKSchool of Biosciences, Cardiff University, Park Place, Cardiff CF10 3AX, UKLaboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USALaboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USASchool of Biosciences, Cardiff University, Park Place, Cardiff CF10 3AX, UKSchool of Medicine, Cardiff University, Heath Campus, Cardiff CF14 4XN, UKSchool of Biosciences, Cardiff University, Park Place, Cardiff CF10 3AX, UKThe Hbl toxin is a three-component haemolytic complex produced by <i>Bacillus cereus sensu lato</i> strains and implicated as a cause of diarrhoea in <i>B. cereus</i> food poisoning. While the structure of the HblB component of this toxin is known, the structures of the other components are unresolved. Here, we describe the expression of the recombinant HblL<sub>1</sub> component and the elucidation of its structure to 1.36 Å. Like HblB, it is a member of the alpha-helical pore-forming toxin family. In comparison to other members of this group, it has an extended hydrophobic beta tongue region that may be involved in pore formation. Molecular docking was used to predict possible interactions between HblL<sub>1</sub> and HblB, and suggests a head to tail dimer might form, burying the HblL<sub>1</sub> beta tongue region.https://www.mdpi.com/2072-6651/13/4/253tripartite toxinhemolytic toxinhaemolytic toxin<i>Bacillus cereus</i> |
| spellingShingle | Harley L. Worthy Lainey J. Williamson Husam Sabah Auhim Stephen H. Leppla Inka Sastalla D. Dafydd Jones Pierre J. Rizkallah Colin Berry The Crystal Structure of <i>Bacillus cereus</i> HblL<sub>1</sub> Toxins tripartite toxin hemolytic toxin haemolytic toxin <i>Bacillus cereus</i> |
| title | The Crystal Structure of <i>Bacillus cereus</i> HblL<sub>1</sub> |
| title_full | The Crystal Structure of <i>Bacillus cereus</i> HblL<sub>1</sub> |
| title_fullStr | The Crystal Structure of <i>Bacillus cereus</i> HblL<sub>1</sub> |
| title_full_unstemmed | The Crystal Structure of <i>Bacillus cereus</i> HblL<sub>1</sub> |
| title_short | The Crystal Structure of <i>Bacillus cereus</i> HblL<sub>1</sub> |
| title_sort | crystal structure of i bacillus cereus i hbll sub 1 sub |
| topic | tripartite toxin hemolytic toxin haemolytic toxin <i>Bacillus cereus</i> |
| url | https://www.mdpi.com/2072-6651/13/4/253 |
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