In Vitro Micropropagation of Commercial Ginseng Cultivars (<i>Panax ginseng</i> Meyer) via Somatic Embryogenesis Compared to Traditional Seed Production
<i>Panax ginseng</i> Meyer is an important medicinal crop; however, most ginseng farmers cultivate native species that are not genetically fixed. Although several cultivars have been developed in Korea, distribution to farmers remains insufficient given their low propagation characterist...
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MDPI AG
2023-03-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/4/435 |
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| author | Jung-Woo Lee Nayeong Kwon Jang-Uk Kim Kyong-Hwan Bang Sung Min Jung Sung Woo Lee Dong-Hwi Kim Young-Chang Kim Ick-Hyun Jo Young-Doo Park |
| author_facet | Jung-Woo Lee Nayeong Kwon Jang-Uk Kim Kyong-Hwan Bang Sung Min Jung Sung Woo Lee Dong-Hwi Kim Young-Chang Kim Ick-Hyun Jo Young-Doo Park |
| author_sort | Jung-Woo Lee |
| collection | DOAJ |
| description | <i>Panax ginseng</i> Meyer is an important medicinal crop; however, most ginseng farmers cultivate native species that are not genetically fixed. Although several cultivars have been developed in Korea, distribution to farmers remains insufficient given their low propagation characteristics. This study compared the efficiency of seed production and micropropagation via somatic embryogenesis. Seeds were collected from cultivars, and zygotic embryo-derived explants were inoculated and cultured in a series of media for micropropagation. Seed production and characteristics of commercial cultivars were evaluated. The number of seeds from a 4-year-old individual cultivar was 23.1–58.8, and seed characteristics varied with cultivars. The genotype had a notable effect on somatic embryogenesis and plant regeneration. The number of somatic embryos and shoots obtained from a single seed of cultivars was 71.3–100.2 and 50.7–61.3, respectively. The number of in vitro grown roots (IGRs) per a single seed was 37.1–41.1 in one year. IGRs were successfully acclimatized and sprouted normally in the field. Flow cytometry analysis suggested that micropropagated plants had no ploidy variations. The results demonstrated the utility of somatic embryogenesis in the in vitro micropropagation of <i>P. ginseng</i> cultivars. Our findings can enhance the distribution of cultivars among farmers in the future. |
| first_indexed | 2024-03-11T04:58:19Z |
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| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-11T04:58:19Z |
| publishDate | 2023-03-01 |
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| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-b0dd3530194d4dd2aef0d03f6f7b08602023-11-17T19:28:41ZengMDPI AGHorticulturae2311-75242023-03-019443510.3390/horticulturae9040435In Vitro Micropropagation of Commercial Ginseng Cultivars (<i>Panax ginseng</i> Meyer) via Somatic Embryogenesis Compared to Traditional Seed ProductionJung-Woo Lee0Nayeong Kwon1Jang-Uk Kim2Kyong-Hwan Bang3Sung Min Jung4Sung Woo Lee5Dong-Hwi Kim6Young-Chang Kim7Ick-Hyun Jo8Young-Doo Park9Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Republic of KoreaDepartment of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Republic of KoreaDepartment of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Republic of KoreaDepartment of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Republic of KoreaDepartment of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Republic of KoreaDepartment of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Republic of KoreaDepartment of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Republic of KoreaResearch Policy Bureau, Rural Development Administration, Jeonju 54875, Republic of KoreaDepartment of Crop Science and Biotechnology, Dankook University, Cheonan 31116, Republic of KoreaDepartment of Horticultural Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea<i>Panax ginseng</i> Meyer is an important medicinal crop; however, most ginseng farmers cultivate native species that are not genetically fixed. Although several cultivars have been developed in Korea, distribution to farmers remains insufficient given their low propagation characteristics. This study compared the efficiency of seed production and micropropagation via somatic embryogenesis. Seeds were collected from cultivars, and zygotic embryo-derived explants were inoculated and cultured in a series of media for micropropagation. Seed production and characteristics of commercial cultivars were evaluated. The number of seeds from a 4-year-old individual cultivar was 23.1–58.8, and seed characteristics varied with cultivars. The genotype had a notable effect on somatic embryogenesis and plant regeneration. The number of somatic embryos and shoots obtained from a single seed of cultivars was 71.3–100.2 and 50.7–61.3, respectively. The number of in vitro grown roots (IGRs) per a single seed was 37.1–41.1 in one year. IGRs were successfully acclimatized and sprouted normally in the field. Flow cytometry analysis suggested that micropropagated plants had no ploidy variations. The results demonstrated the utility of somatic embryogenesis in the in vitro micropropagation of <i>P. ginseng</i> cultivars. Our findings can enhance the distribution of cultivars among farmers in the future.https://www.mdpi.com/2311-7524/9/4/435<i>Panax ginseng</i> Meyersomatic embryogenesisin vitro grown rootsmicropropagationcommercial propagationmedicinal crop |
| spellingShingle | Jung-Woo Lee Nayeong Kwon Jang-Uk Kim Kyong-Hwan Bang Sung Min Jung Sung Woo Lee Dong-Hwi Kim Young-Chang Kim Ick-Hyun Jo Young-Doo Park In Vitro Micropropagation of Commercial Ginseng Cultivars (<i>Panax ginseng</i> Meyer) via Somatic Embryogenesis Compared to Traditional Seed Production Horticulturae <i>Panax ginseng</i> Meyer somatic embryogenesis in vitro grown roots micropropagation commercial propagation medicinal crop |
| title | In Vitro Micropropagation of Commercial Ginseng Cultivars (<i>Panax ginseng</i> Meyer) via Somatic Embryogenesis Compared to Traditional Seed Production |
| title_full | In Vitro Micropropagation of Commercial Ginseng Cultivars (<i>Panax ginseng</i> Meyer) via Somatic Embryogenesis Compared to Traditional Seed Production |
| title_fullStr | In Vitro Micropropagation of Commercial Ginseng Cultivars (<i>Panax ginseng</i> Meyer) via Somatic Embryogenesis Compared to Traditional Seed Production |
| title_full_unstemmed | In Vitro Micropropagation of Commercial Ginseng Cultivars (<i>Panax ginseng</i> Meyer) via Somatic Embryogenesis Compared to Traditional Seed Production |
| title_short | In Vitro Micropropagation of Commercial Ginseng Cultivars (<i>Panax ginseng</i> Meyer) via Somatic Embryogenesis Compared to Traditional Seed Production |
| title_sort | in vitro micropropagation of commercial ginseng cultivars i panax ginseng i meyer via somatic embryogenesis compared to traditional seed production |
| topic | <i>Panax ginseng</i> Meyer somatic embryogenesis in vitro grown roots micropropagation commercial propagation medicinal crop |
| url | https://www.mdpi.com/2311-7524/9/4/435 |
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