Degradation of azo dye by bacterium, Alishewanella sp. CBL-2 isolated from industrial effluent and its potential use in decontamination of wastewater
In the present study, Alishewanella sp. CBL-2 was characterized on the basis of biochemical and 16S rRNA sequencing. The bacterium was found to decolorize 83% Sumifex Tourqi blue within a period of 6 days at 37 °C and pH 7. The predominant form of the enzyme was found to be extracellular (78%) as co...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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IWA Publishing
2018-12-01
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| Series: | Journal of Water Reuse and Desalination |
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| Online Access: | http://jwrd.iwaponline.com/content/8/4/507 |
| _version_ | 1819226621579624448 |
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| author | Mehvish Ajaz Amina Elahi Abdul Rehman |
| author_facet | Mehvish Ajaz Amina Elahi Abdul Rehman |
| author_sort | Mehvish Ajaz |
| collection | DOAJ |
| description | In the present study, Alishewanella sp. CBL-2 was characterized on the basis of biochemical and 16S rRNA sequencing. The bacterium was found to decolorize 83% Sumifex Tourqi blue within a period of 6 days at 37 °C and pH 7. The predominant form of the enzyme was found to be extracellular (78%) as compared to intracellular (16%). The breakdown of azo bond was confirmed through thin layer chromatography (TLC) analysis as new bands and peaks were observed in chromatograms of extracted metabolites of decolorized samples. Growth of beneficial microbial fauna in the presence of bacterial decolorized wastewater indicates that this wastewater is safe for the survival of microorganisms. High bioremediation potential makes Alishewanella sp. CBL-2 an impending foundation for green chemistry to remove azo dyes from industrial wastewater. |
| first_indexed | 2024-12-23T10:28:24Z |
| format | Article |
| id | doaj.art-b0de596204a847859a56c534ce14ef40 |
| institution | Directory Open Access Journal |
| issn | 2220-1319 2408-9370 |
| language | English |
| last_indexed | 2024-12-23T10:28:24Z |
| publishDate | 2018-12-01 |
| publisher | IWA Publishing |
| record_format | Article |
| series | Journal of Water Reuse and Desalination |
| spelling | doaj.art-b0de596204a847859a56c534ce14ef402022-12-21T17:50:29ZengIWA PublishingJournal of Water Reuse and Desalination2220-13192408-93702018-12-018450751510.2166/wrd.2018.065065Degradation of azo dye by bacterium, Alishewanella sp. CBL-2 isolated from industrial effluent and its potential use in decontamination of wastewaterMehvish Ajaz0Amina Elahi1Abdul Rehman2 Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Lahore 54590, Pakistan Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Lahore 54590, Pakistan Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Lahore 54590, Pakistan In the present study, Alishewanella sp. CBL-2 was characterized on the basis of biochemical and 16S rRNA sequencing. The bacterium was found to decolorize 83% Sumifex Tourqi blue within a period of 6 days at 37 °C and pH 7. The predominant form of the enzyme was found to be extracellular (78%) as compared to intracellular (16%). The breakdown of azo bond was confirmed through thin layer chromatography (TLC) analysis as new bands and peaks were observed in chromatograms of extracted metabolites of decolorized samples. Growth of beneficial microbial fauna in the presence of bacterial decolorized wastewater indicates that this wastewater is safe for the survival of microorganisms. High bioremediation potential makes Alishewanella sp. CBL-2 an impending foundation for green chemistry to remove azo dyes from industrial wastewater.http://jwrd.iwaponline.com/content/8/4/507Alishewanella sp. CBL-2azo dyedecolorizationTLC |
| spellingShingle | Mehvish Ajaz Amina Elahi Abdul Rehman Degradation of azo dye by bacterium, Alishewanella sp. CBL-2 isolated from industrial effluent and its potential use in decontamination of wastewater Journal of Water Reuse and Desalination Alishewanella sp. CBL-2 azo dye decolorization TLC |
| title | Degradation of azo dye by bacterium, Alishewanella sp. CBL-2 isolated from industrial effluent and its potential use in decontamination of wastewater |
| title_full | Degradation of azo dye by bacterium, Alishewanella sp. CBL-2 isolated from industrial effluent and its potential use in decontamination of wastewater |
| title_fullStr | Degradation of azo dye by bacterium, Alishewanella sp. CBL-2 isolated from industrial effluent and its potential use in decontamination of wastewater |
| title_full_unstemmed | Degradation of azo dye by bacterium, Alishewanella sp. CBL-2 isolated from industrial effluent and its potential use in decontamination of wastewater |
| title_short | Degradation of azo dye by bacterium, Alishewanella sp. CBL-2 isolated from industrial effluent and its potential use in decontamination of wastewater |
| title_sort | degradation of azo dye by bacterium alishewanella sp cbl 2 isolated from industrial effluent and its potential use in decontamination of wastewater |
| topic | Alishewanella sp. CBL-2 azo dye decolorization TLC |
| url | http://jwrd.iwaponline.com/content/8/4/507 |
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