Isolation and characterization of bioactive lactoferrin from camel milk by novel pH-dependent method for large scale production
The present article exemplifies a novel method to isolate highly purified bioactive lactoferrin from camel milk. Cytotoxicity of lactoferrin against the Hela cells was used to evaluate its bioactivity. SDS-PAGE and LC-MS analysis was done for its identification and characterization. The purified cam...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Biotechnology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X22000637 |
| _version_ | 1811258555560361984 |
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| author | Neelam Mahala Aastha Mittal Manohar Lal Uma S. Dubey |
| author_facet | Neelam Mahala Aastha Mittal Manohar Lal Uma S. Dubey |
| author_sort | Neelam Mahala |
| collection | DOAJ |
| description | The present article exemplifies a novel method to isolate highly purified bioactive lactoferrin from camel milk. Cytotoxicity of lactoferrin against the Hela cells was used to evaluate its bioactivity. SDS-PAGE and LC-MS analysis was done for its identification and characterization. The purified camel milk lactoferrin was found to be 708 amino acids in length with a molecular weight of 77.3 kDa and a pI value of 8.24. This pH-dependent isolation procedure ensures the retention of bioactive lactoferrin from camel milk. The importance of the present work lies in its simplicity and scalability for manufacturing bioactive lactoferrin at an industrial level. |
| first_indexed | 2024-04-12T18:16:13Z |
| format | Article |
| id | doaj.art-b0e0b4a4a07c4d4aaf7313380ceb0e08 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-04-12T18:16:13Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-b0e0b4a4a07c4d4aaf7313380ceb0e082022-12-22T03:21:37ZengElsevierBiotechnology Reports2215-017X2022-12-0136e00765Isolation and characterization of bioactive lactoferrin from camel milk by novel pH-dependent method for large scale productionNeelam Mahala0Aastha Mittal1Manohar Lal2Uma S. Dubey3Department of Biological Sciences, Birla Institute of Technology of Science, Pilani (BITS Pilani), Pilani Campus, Rajasthan 333031, IndiaDepartment of Biological Sciences, Birla Institute of Technology of Science, Pilani (BITS Pilani), Pilani Campus, Rajasthan 333031, IndiaDepartment of Biological Sciences, Birla Institute of Technology of Science, Pilani (BITS Pilani), Pilani Campus, Rajasthan 333031, IndiaCorresponding author.; Department of Biological Sciences, Birla Institute of Technology of Science, Pilani (BITS Pilani), Pilani Campus, Rajasthan 333031, IndiaThe present article exemplifies a novel method to isolate highly purified bioactive lactoferrin from camel milk. Cytotoxicity of lactoferrin against the Hela cells was used to evaluate its bioactivity. SDS-PAGE and LC-MS analysis was done for its identification and characterization. The purified camel milk lactoferrin was found to be 708 amino acids in length with a molecular weight of 77.3 kDa and a pI value of 8.24. This pH-dependent isolation procedure ensures the retention of bioactive lactoferrin from camel milk. The importance of the present work lies in its simplicity and scalability for manufacturing bioactive lactoferrin at an industrial level.http://www.sciencedirect.com/science/article/pii/S2215017X22000637Camel milkWheyLactoferrinpH-dependent isolationLiquid chromatography-mass spectrometry (LC-MS)Cytotoxicity assay |
| spellingShingle | Neelam Mahala Aastha Mittal Manohar Lal Uma S. Dubey Isolation and characterization of bioactive lactoferrin from camel milk by novel pH-dependent method for large scale production Biotechnology Reports Camel milk Whey Lactoferrin pH-dependent isolation Liquid chromatography-mass spectrometry (LC-MS) Cytotoxicity assay |
| title | Isolation and characterization of bioactive lactoferrin from camel milk by novel pH-dependent method for large scale production |
| title_full | Isolation and characterization of bioactive lactoferrin from camel milk by novel pH-dependent method for large scale production |
| title_fullStr | Isolation and characterization of bioactive lactoferrin from camel milk by novel pH-dependent method for large scale production |
| title_full_unstemmed | Isolation and characterization of bioactive lactoferrin from camel milk by novel pH-dependent method for large scale production |
| title_short | Isolation and characterization of bioactive lactoferrin from camel milk by novel pH-dependent method for large scale production |
| title_sort | isolation and characterization of bioactive lactoferrin from camel milk by novel ph dependent method for large scale production |
| topic | Camel milk Whey Lactoferrin pH-dependent isolation Liquid chromatography-mass spectrometry (LC-MS) Cytotoxicity assay |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X22000637 |
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