Characterisation of Collagen Re-Modelling in Localised Prostate Cancer Using Second-Generation Harmonic Imaging and Transrectal Ultrasound Shear Wave Elastography
Prostate cancer has a poor prognosis and high mortality rate due to metastases. Extracellular matrix (ECM) re-modelling and stroma composition have been linked to cancer progression, including key components of cell migration, tumour metastasis, and tissue modulus. Moreover, collagens are one of the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/13/21/5553 |
| _version_ | 1797512729946226688 |
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| author | Wael Ageeli Xinyu Zhang Chidozie N. Ogbonnaya Yuting Ling Jennifer Wilson Chunhui Li Ghulam Nabi |
| author_facet | Wael Ageeli Xinyu Zhang Chidozie N. Ogbonnaya Yuting Ling Jennifer Wilson Chunhui Li Ghulam Nabi |
| author_sort | Wael Ageeli |
| collection | DOAJ |
| description | Prostate cancer has a poor prognosis and high mortality rate due to metastases. Extracellular matrix (ECM) re-modelling and stroma composition have been linked to cancer progression, including key components of cell migration, tumour metastasis, and tissue modulus. Moreover, collagens are one of the most significant components of the extracellular matrix and have been ascribed to many aspects of neoplastic transformation. This study characterises collagen re-modelling around localised prostate cancer using the second harmonic generation of collagen (SHG), genotyping and ultrasound shear wave elastography (USWE) measured modulus in men with clinically localised prostate cancer. Tempo-sequence assay for gene expression of COL1A1 and COL3A1 was used to confirm the expression of collagen. Second-harmonic generation imaging and genotyping of ECM around prostate cancer showed changes in content, orientation, and type of collagen according to Gleason grades (cancer aggressivity), and this correlated with the tissue modulus measured by USWE in kilopascals. Furthermore, there were clear differences between collagen orientation and type around normal and cancer tissues. |
| first_indexed | 2024-03-10T06:04:53Z |
| format | Article |
| id | doaj.art-b0e23d298f2b47ccb60e4ea65d6c56d3 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T06:04:53Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-b0e23d298f2b47ccb60e4ea65d6c56d32023-11-22T20:36:57ZengMDPI AGCancers2072-66942021-11-011321555310.3390/cancers13215553Characterisation of Collagen Re-Modelling in Localised Prostate Cancer Using Second-Generation Harmonic Imaging and Transrectal Ultrasound Shear Wave ElastographyWael Ageeli0Xinyu Zhang1Chidozie N. Ogbonnaya2Yuting Ling3Jennifer Wilson4Chunhui Li5Ghulam Nabi6Division of Imaging Sciences and Technology, School of Medicine, University of Dundee, Ninewells Hospital, Dundee DD1 9SY, UKDivision of Population Health and Genomics, School of Medicine, University of Dundee, Dundee DD1 9SY, UKDivision of Imaging Sciences and Technology, School of Medicine, University of Dundee, Ninewells Hospital, Dundee DD1 9SY, UKImaging Science and Technology, School of Medicine, University of Dundee, Dundee DD1 9SY, UKDepartment of Pathology, Ninewells Hospital, Dundee DD1 9SY, UKSchool of Science and Engineering, University of Dundee, Dundee DD1 4HN, UKDivision of Imaging Sciences and Technology, School of Medicine, University of Dundee, Ninewells Hospital, Dundee DD1 9SY, UKProstate cancer has a poor prognosis and high mortality rate due to metastases. Extracellular matrix (ECM) re-modelling and stroma composition have been linked to cancer progression, including key components of cell migration, tumour metastasis, and tissue modulus. Moreover, collagens are one of the most significant components of the extracellular matrix and have been ascribed to many aspects of neoplastic transformation. This study characterises collagen re-modelling around localised prostate cancer using the second harmonic generation of collagen (SHG), genotyping and ultrasound shear wave elastography (USWE) measured modulus in men with clinically localised prostate cancer. Tempo-sequence assay for gene expression of COL1A1 and COL3A1 was used to confirm the expression of collagen. Second-harmonic generation imaging and genotyping of ECM around prostate cancer showed changes in content, orientation, and type of collagen according to Gleason grades (cancer aggressivity), and this correlated with the tissue modulus measured by USWE in kilopascals. Furthermore, there were clear differences between collagen orientation and type around normal and cancer tissues.https://www.mdpi.com/2072-6694/13/21/5553prostatecollagensecond-generation harmonicsshear waveelastographyultrasound |
| spellingShingle | Wael Ageeli Xinyu Zhang Chidozie N. Ogbonnaya Yuting Ling Jennifer Wilson Chunhui Li Ghulam Nabi Characterisation of Collagen Re-Modelling in Localised Prostate Cancer Using Second-Generation Harmonic Imaging and Transrectal Ultrasound Shear Wave Elastography Cancers prostate collagen second-generation harmonics shear wave elastography ultrasound |
| title | Characterisation of Collagen Re-Modelling in Localised Prostate Cancer Using Second-Generation Harmonic Imaging and Transrectal Ultrasound Shear Wave Elastography |
| title_full | Characterisation of Collagen Re-Modelling in Localised Prostate Cancer Using Second-Generation Harmonic Imaging and Transrectal Ultrasound Shear Wave Elastography |
| title_fullStr | Characterisation of Collagen Re-Modelling in Localised Prostate Cancer Using Second-Generation Harmonic Imaging and Transrectal Ultrasound Shear Wave Elastography |
| title_full_unstemmed | Characterisation of Collagen Re-Modelling in Localised Prostate Cancer Using Second-Generation Harmonic Imaging and Transrectal Ultrasound Shear Wave Elastography |
| title_short | Characterisation of Collagen Re-Modelling in Localised Prostate Cancer Using Second-Generation Harmonic Imaging and Transrectal Ultrasound Shear Wave Elastography |
| title_sort | characterisation of collagen re modelling in localised prostate cancer using second generation harmonic imaging and transrectal ultrasound shear wave elastography |
| topic | prostate collagen second-generation harmonics shear wave elastography ultrasound |
| url | https://www.mdpi.com/2072-6694/13/21/5553 |
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