Immune infiltrating cells in cholangiocarcinoma may become clinical diagnostic markers: based on bioinformatics analysis
Abstract Background Intrahepatic cholangiocarcinoma (ICC) is a malignant tumor originating from the secondary bile duct and its branch epithelium. Among primary liver tumors, the incidence of ICC is second only to hepatocellular carcinoma. Tumor microenvironment can regulate the occurrence and devel...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-02-01
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| Series: | World Journal of Surgical Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12957-021-02168-8 |
| _version_ | 1818849887491457024 |
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| author | Yongwei Zhang Sihan Chen Jun Li Wei Dai Yeben Qian |
| author_facet | Yongwei Zhang Sihan Chen Jun Li Wei Dai Yeben Qian |
| author_sort | Yongwei Zhang |
| collection | DOAJ |
| description | Abstract Background Intrahepatic cholangiocarcinoma (ICC) is a malignant tumor originating from the secondary bile duct and its branch epithelium. Among primary liver tumors, the incidence of ICC is second only to hepatocellular carcinoma. Tumor microenvironment can regulate the occurrence and development of tumors. This study is dedicated to finding more markers that can diagnose ICC by finding the differential tumor microenvironment cells between ICC and normal tissues. Methods We wanted to study the infiltration of immune cells between the cholangiocarcinoma of the same patient and its paired non-tumor tissues, to explore the difference of immune cells in the tumor microenvironment and adjacent non-tumor tissues in the same organism. So, we searched the relevant data of patients with ICC from the GEO database and found that the GSE45001 data set meets our research needs. CIBERSORT database is used to calculate immune cell composition. Finally, perform visual analysis and data statistics to find out the differentially expressed immune cells. Results We found that the expression levels of dendritic cells activated, macrophages M2, and T cells regulatory (Tregs) in ICC were higher than normal tissues, and the expression levels of macrophages M1, monocytes, and T cells follicular helper in ICC were lower than normal tissues. Conclusion These 6 types of immune cells are expected to become molecular markers for clinical diagnosis of ICC. |
| first_indexed | 2024-12-19T06:40:23Z |
| format | Article |
| id | doaj.art-b0e9e3bc2594418dabe13c5717a3593b |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-12-19T06:40:23Z |
| publishDate | 2021-02-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-b0e9e3bc2594418dabe13c5717a3593b2022-12-21T20:32:06ZengBMCWorld Journal of Surgical Oncology1477-78192021-02-011911810.1186/s12957-021-02168-8Immune infiltrating cells in cholangiocarcinoma may become clinical diagnostic markers: based on bioinformatics analysisYongwei Zhang0Sihan Chen1Jun Li2Wei Dai3Yeben Qian4Department of General Surgery, The First Affiliated Hospital of Anhui Medical UniversityDepartment of General Surgery, The First Affiliated Hospital of Anhui Medical UniversityDepartment of General Surgery, The First Affiliated Hospital of Anhui Medical UniversityDepartment of General Surgery, The First Affiliated Hospital of Anhui Medical UniversityDepartment of General Surgery, The First Affiliated Hospital of Anhui Medical UniversityAbstract Background Intrahepatic cholangiocarcinoma (ICC) is a malignant tumor originating from the secondary bile duct and its branch epithelium. Among primary liver tumors, the incidence of ICC is second only to hepatocellular carcinoma. Tumor microenvironment can regulate the occurrence and development of tumors. This study is dedicated to finding more markers that can diagnose ICC by finding the differential tumor microenvironment cells between ICC and normal tissues. Methods We wanted to study the infiltration of immune cells between the cholangiocarcinoma of the same patient and its paired non-tumor tissues, to explore the difference of immune cells in the tumor microenvironment and adjacent non-tumor tissues in the same organism. So, we searched the relevant data of patients with ICC from the GEO database and found that the GSE45001 data set meets our research needs. CIBERSORT database is used to calculate immune cell composition. Finally, perform visual analysis and data statistics to find out the differentially expressed immune cells. Results We found that the expression levels of dendritic cells activated, macrophages M2, and T cells regulatory (Tregs) in ICC were higher than normal tissues, and the expression levels of macrophages M1, monocytes, and T cells follicular helper in ICC were lower than normal tissues. Conclusion These 6 types of immune cells are expected to become molecular markers for clinical diagnosis of ICC.https://doi.org/10.1186/s12957-021-02168-8Bioinformatics analysisPrognosisIntrahepatic cholangiocarcinomaImmune cells |
| spellingShingle | Yongwei Zhang Sihan Chen Jun Li Wei Dai Yeben Qian Immune infiltrating cells in cholangiocarcinoma may become clinical diagnostic markers: based on bioinformatics analysis World Journal of Surgical Oncology Bioinformatics analysis Prognosis Intrahepatic cholangiocarcinoma Immune cells |
| title | Immune infiltrating cells in cholangiocarcinoma may become clinical diagnostic markers: based on bioinformatics analysis |
| title_full | Immune infiltrating cells in cholangiocarcinoma may become clinical diagnostic markers: based on bioinformatics analysis |
| title_fullStr | Immune infiltrating cells in cholangiocarcinoma may become clinical diagnostic markers: based on bioinformatics analysis |
| title_full_unstemmed | Immune infiltrating cells in cholangiocarcinoma may become clinical diagnostic markers: based on bioinformatics analysis |
| title_short | Immune infiltrating cells in cholangiocarcinoma may become clinical diagnostic markers: based on bioinformatics analysis |
| title_sort | immune infiltrating cells in cholangiocarcinoma may become clinical diagnostic markers based on bioinformatics analysis |
| topic | Bioinformatics analysis Prognosis Intrahepatic cholangiocarcinoma Immune cells |
| url | https://doi.org/10.1186/s12957-021-02168-8 |
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