Generation of miR-15a/16-1 cluster-deficient human induced pluripotent stem cell line (DMBi001-A-2) using CRISPR/Cas9 gene editing
miR-15a/16-1 cluster, composed of MIR15A and MIR16-1 genes located in close proximity on chromosome 13 was described to regulate post-natal cell cycle withdrawal of cardiomyocytes in mice. In humans, on the other hand, the level of miR-15a-5p and miR-16-p was negatively associated with the severity...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Stem Cell Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506123000326 |
| _version_ | 1797870608927686656 |
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| author | Jacek Stępniewski Mateusz Jeż Józef Dulak |
| author_facet | Jacek Stępniewski Mateusz Jeż Józef Dulak |
| author_sort | Jacek Stępniewski |
| collection | DOAJ |
| description | miR-15a/16-1 cluster, composed of MIR15A and MIR16-1 genes located in close proximity on chromosome 13 was described to regulate post-natal cell cycle withdrawal of cardiomyocytes in mice. In humans, on the other hand, the level of miR-15a-5p and miR-16-p was negatively associated with the severity of cardiac hypertrophy. Therefore, to better understand the role of these microRNAs in human cardiomyocytes in regard to their proliferative potential and hypertrophic growth, we generated hiPSC line with complete deletion of miR-15a/16-1 cluster using CRISPR/Cas9 gene editing. Obtained cells demonstrate expression of pluripotency markers, differentiation capacity into all three germ layers and normal karyotype. |
| first_indexed | 2024-04-10T00:31:10Z |
| format | Article |
| id | doaj.art-b0ec8889b4194714b81ed9bc3c17ec32 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-04-10T00:31:10Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-b0ec8889b4194714b81ed9bc3c17ec322023-03-15T04:27:29ZengElsevierStem Cell Research1873-50612023-04-0168103046Generation of miR-15a/16-1 cluster-deficient human induced pluripotent stem cell line (DMBi001-A-2) using CRISPR/Cas9 gene editingJacek Stępniewski0Mateusz Jeż1Józef Dulak2Corresponding authors.; Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, PolandDepartment of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, PolandCorresponding authors.; Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, PolandmiR-15a/16-1 cluster, composed of MIR15A and MIR16-1 genes located in close proximity on chromosome 13 was described to regulate post-natal cell cycle withdrawal of cardiomyocytes in mice. In humans, on the other hand, the level of miR-15a-5p and miR-16-p was negatively associated with the severity of cardiac hypertrophy. Therefore, to better understand the role of these microRNAs in human cardiomyocytes in regard to their proliferative potential and hypertrophic growth, we generated hiPSC line with complete deletion of miR-15a/16-1 cluster using CRISPR/Cas9 gene editing. Obtained cells demonstrate expression of pluripotency markers, differentiation capacity into all three germ layers and normal karyotype.http://www.sciencedirect.com/science/article/pii/S1873506123000326hiPSCmicroRNA-15amicroRNA-16-1CRISPR/Cas9CardiomyocytesCardiac hypertrophy |
| spellingShingle | Jacek Stępniewski Mateusz Jeż Józef Dulak Generation of miR-15a/16-1 cluster-deficient human induced pluripotent stem cell line (DMBi001-A-2) using CRISPR/Cas9 gene editing Stem Cell Research hiPSC microRNA-15a microRNA-16-1 CRISPR/Cas9 Cardiomyocytes Cardiac hypertrophy |
| title | Generation of miR-15a/16-1 cluster-deficient human induced pluripotent stem cell line (DMBi001-A-2) using CRISPR/Cas9 gene editing |
| title_full | Generation of miR-15a/16-1 cluster-deficient human induced pluripotent stem cell line (DMBi001-A-2) using CRISPR/Cas9 gene editing |
| title_fullStr | Generation of miR-15a/16-1 cluster-deficient human induced pluripotent stem cell line (DMBi001-A-2) using CRISPR/Cas9 gene editing |
| title_full_unstemmed | Generation of miR-15a/16-1 cluster-deficient human induced pluripotent stem cell line (DMBi001-A-2) using CRISPR/Cas9 gene editing |
| title_short | Generation of miR-15a/16-1 cluster-deficient human induced pluripotent stem cell line (DMBi001-A-2) using CRISPR/Cas9 gene editing |
| title_sort | generation of mir 15a 16 1 cluster deficient human induced pluripotent stem cell line dmbi001 a 2 using crispr cas9 gene editing |
| topic | hiPSC microRNA-15a microRNA-16-1 CRISPR/Cas9 Cardiomyocytes Cardiac hypertrophy |
| url | http://www.sciencedirect.com/science/article/pii/S1873506123000326 |
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