Natural Antioxidant Evaluation: A Review of Detection Methods
Antioxidants have drawn the attention of the scientific community due to being related to the prevention of various degenerative diseases. The antioxidant capacity has been extensively studied in vitro, and different methods have been used to assess its activity. However, the main issues related to...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/11/3563 |
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| author | Jenifer da Silva Mendonça Rita de Cássia Avellaneda Guimarães Verônica Assalin Zorgetto-Pinheiro Carolina Di Pietro Fernandes Gabriela Marcelino Danielle Bogo Karine de Cássia Freitas Priscila Aiko Hiane Elaine Silva de Pádua Melo Marcelo Luiz Brandão Vilela Valter Aragão do Nascimento |
| author_facet | Jenifer da Silva Mendonça Rita de Cássia Avellaneda Guimarães Verônica Assalin Zorgetto-Pinheiro Carolina Di Pietro Fernandes Gabriela Marcelino Danielle Bogo Karine de Cássia Freitas Priscila Aiko Hiane Elaine Silva de Pádua Melo Marcelo Luiz Brandão Vilela Valter Aragão do Nascimento |
| author_sort | Jenifer da Silva Mendonça |
| collection | DOAJ |
| description | Antioxidants have drawn the attention of the scientific community due to being related to the prevention of various degenerative diseases. The antioxidant capacity has been extensively studied in vitro, and different methods have been used to assess its activity. However, the main issues related to studying natural antioxidants are evaluating whether these antioxidants demonstrate a key role in the biological system and assessing their bioavailability in the organism. The majority of outcomes in the literature are controversial due to a lack of method standardization and their proper application. Therefore, this study aims to compile the main issues concerning the natural antioxidant field of study, comparing the most common in vitro methods to evaluate the antioxidant activity of natural compounds, demonstrating the antioxidant activity in biological systems and the role of the main antioxidant enzymes of redox cellular signaling and explaining how the bioavailability of bioactive compounds is evaluated in animal models and human clinical trials. |
| first_indexed | 2024-03-10T01:03:46Z |
| format | Article |
| id | doaj.art-b0eefe43b56342b4b8f11ff458af0799 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T01:03:46Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b0eefe43b56342b4b8f11ff458af07992023-11-23T14:30:49ZengMDPI AGMolecules1420-30492022-06-012711356310.3390/molecules27113563Natural Antioxidant Evaluation: A Review of Detection MethodsJenifer da Silva Mendonça0Rita de Cássia Avellaneda Guimarães1Verônica Assalin Zorgetto-Pinheiro2Carolina Di Pietro Fernandes3Gabriela Marcelino4Danielle Bogo5Karine de Cássia Freitas6Priscila Aiko Hiane7Elaine Silva de Pádua Melo8Marcelo Luiz Brandão Vilela9Valter Aragão do Nascimento10Graduate Program in Health and Development in the Central-West Region of Brazil, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, BrazilGraduate Program in Health and Development in the Central-West Region of Brazil, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, BrazilGraduate Program in Health and Development in the Central-West Region of Brazil, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, BrazilGroup of Spectroscopy and Bioinformatics Applied Biodiversity and Health (GEBABS), Federal University of Mato Grosso do Sul, Campo Grande 79070-900, BrazilGraduate Program in Health and Development in the Central-West Region of Brazil, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, BrazilGraduate Program in Health and Development in the Central-West Region of Brazil, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, BrazilGraduate Program in Health and Development in the Central-West Region of Brazil, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, BrazilGraduate Program in Health and Development in the Central-West Region of Brazil, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, BrazilGraduate Program in Health and Development in the Central-West Region of Brazil, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, BrazilSchool of Medicine, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, BrazilGraduate Program in Health and Development in the Central-West Region of Brazil, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, BrazilAntioxidants have drawn the attention of the scientific community due to being related to the prevention of various degenerative diseases. The antioxidant capacity has been extensively studied in vitro, and different methods have been used to assess its activity. However, the main issues related to studying natural antioxidants are evaluating whether these antioxidants demonstrate a key role in the biological system and assessing their bioavailability in the organism. The majority of outcomes in the literature are controversial due to a lack of method standardization and their proper application. Therefore, this study aims to compile the main issues concerning the natural antioxidant field of study, comparing the most common in vitro methods to evaluate the antioxidant activity of natural compounds, demonstrating the antioxidant activity in biological systems and the role of the main antioxidant enzymes of redox cellular signaling and explaining how the bioavailability of bioactive compounds is evaluated in animal models and human clinical trials.https://www.mdpi.com/1420-3049/27/11/3563antioxidantbioactive compoundssecondary metabolitesantioxidant assayin vitro modelsin vivo models |
| spellingShingle | Jenifer da Silva Mendonça Rita de Cássia Avellaneda Guimarães Verônica Assalin Zorgetto-Pinheiro Carolina Di Pietro Fernandes Gabriela Marcelino Danielle Bogo Karine de Cássia Freitas Priscila Aiko Hiane Elaine Silva de Pádua Melo Marcelo Luiz Brandão Vilela Valter Aragão do Nascimento Natural Antioxidant Evaluation: A Review of Detection Methods Molecules antioxidant bioactive compounds secondary metabolites antioxidant assay in vitro models in vivo models |
| title | Natural Antioxidant Evaluation: A Review of Detection Methods |
| title_full | Natural Antioxidant Evaluation: A Review of Detection Methods |
| title_fullStr | Natural Antioxidant Evaluation: A Review of Detection Methods |
| title_full_unstemmed | Natural Antioxidant Evaluation: A Review of Detection Methods |
| title_short | Natural Antioxidant Evaluation: A Review of Detection Methods |
| title_sort | natural antioxidant evaluation a review of detection methods |
| topic | antioxidant bioactive compounds secondary metabolites antioxidant assay in vitro models in vivo models |
| url | https://www.mdpi.com/1420-3049/27/11/3563 |
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