Trypanosoma cruzi strain TcI is associated with chronic Chagas disease in the Brazilian Amazon
Abstract Background Chagas disease in the Amazon region is considered an emerging anthropozoonosis with a predominance of the discrete typing units (DTUs) TcI and TcIV. These DTUs are responsible for cases of acute disease associated with oral transmission. Chronic disease cases have been detected t...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2014-06-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/1756-3305-7-267 |
| _version_ | 1797811774882316288 |
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| author | Rosa Amélia Gonçalves Santana Laylah Kelre Costa Magalhães Laise Kelman Costa Magalhães Suzane Ribeiro Prestes Marcel Gonçalves Maciel George Allan Villarouco da Silva Wuelton Marcelo Monteiro Felipe Rocha de Brito Leila Inês de Aguiar Raposo Câmara Coelho João Marcos Barbosa-Ferreira Jorge Augusto Oliveira Guerra Henrique Silveira Maria das Graças Vale Barbosa |
| author_facet | Rosa Amélia Gonçalves Santana Laylah Kelre Costa Magalhães Laise Kelman Costa Magalhães Suzane Ribeiro Prestes Marcel Gonçalves Maciel George Allan Villarouco da Silva Wuelton Marcelo Monteiro Felipe Rocha de Brito Leila Inês de Aguiar Raposo Câmara Coelho João Marcos Barbosa-Ferreira Jorge Augusto Oliveira Guerra Henrique Silveira Maria das Graças Vale Barbosa |
| author_sort | Rosa Amélia Gonçalves Santana |
| collection | DOAJ |
| description | Abstract Background Chagas disease in the Amazon region is considered an emerging anthropozoonosis with a predominance of the discrete typing units (DTUs) TcI and TcIV. These DTUs are responsible for cases of acute disease associated with oral transmission. Chronic disease cases have been detected through serological surveys. However, the mode of transmission could not be determined, or any association of chronic disease with a specific T. cruzi DTU’s. The aim of this study was to characterize Trypanosoma cruzi in patients with chronic Chagas disease in the State of Amazonas, Brazil. Methods Blood culture and xenodiagnosis were performed in 36 patients with positive serology for Chagas disease who participated in a serological survey performed in urban and rural areas of Manaus, Amazonas. DNA samples were extracted from the feces of triatomines used for xenodiagnosis, and the nontranscribed spacer of the mini-exon gene and the mitochondrial gene cytochrome oxidase subunit II (COII) were amplified by PCR and sequenced. Results Blood culture and xenodiagnosis were negative in 100% of samples; however, molecular techniques revealed that in 13 out of 36 (36%) fecal samples from xenodiagnosis, T. cruzi was characterized as the DTU TcI, and different haplotypes were identified within the same DTU. Conclusion The DTU TcI, which is mainly associated with acute cases of Chagas disease in the Amazon region, is also responsible for chronic infection in patients from a region in the State of Amazonas. |
| first_indexed | 2024-03-13T07:27:41Z |
| format | Article |
| id | doaj.art-b0f15cb39c294947b2ea7d1e5754ffff |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:27:41Z |
| publishDate | 2014-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-b0f15cb39c294947b2ea7d1e5754ffff2023-06-04T11:17:13ZengBMCParasites & Vectors1756-33052014-06-01711710.1186/1756-3305-7-267Trypanosoma cruzi strain TcI is associated with chronic Chagas disease in the Brazilian AmazonRosa Amélia Gonçalves Santana0Laylah Kelre Costa Magalhães1Laise Kelman Costa Magalhães2Suzane Ribeiro Prestes3Marcel Gonçalves Maciel4George Allan Villarouco da Silva5Wuelton Marcelo Monteiro6Felipe Rocha de Brito7Leila Inês de Aguiar Raposo Câmara Coelho8João Marcos Barbosa-Ferreira9Jorge Augusto Oliveira Guerra10Henrique Silveira11Maria das Graças Vale Barbosa12University of the State of Amazonas (Universidade do Estado do Amazonas)University of the State of Amazonas (Universidade do Estado do Amazonas)University of the State of Amazonas (Universidade do Estado do Amazonas)University of the State of Amazonas (Universidade do Estado do Amazonas)University of the State of Amazonas (Universidade do Estado do Amazonas)Leônidas and Maria Deane InstituteUniversity of the State of Amazonas (Universidade do Estado do Amazonas)Heitor Vieira Dourado Tropical Medicine Foundation (Fundação de Medicina Tropical Heitor Vieira Dourado)Federal University of Amazonas (Universidade Federal do Amazonas)University of the State of Amazonas (Universidade do Estado do Amazonas)Heitor Vieira Dourado Tropical Medicine Foundation (Fundação de Medicina Tropical Heitor Vieira Dourado)Institute of Hygiene and Tropical Medicine (Instituto de Higiene e Medicina Tropical), New University of Lisbon (Universidade Nova de Lisboa)University of the State of Amazonas (Universidade do Estado do Amazonas)Abstract Background Chagas disease in the Amazon region is considered an emerging anthropozoonosis with a predominance of the discrete typing units (DTUs) TcI and TcIV. These DTUs are responsible for cases of acute disease associated with oral transmission. Chronic disease cases have been detected through serological surveys. However, the mode of transmission could not be determined, or any association of chronic disease with a specific T. cruzi DTU’s. The aim of this study was to characterize Trypanosoma cruzi in patients with chronic Chagas disease in the State of Amazonas, Brazil. Methods Blood culture and xenodiagnosis were performed in 36 patients with positive serology for Chagas disease who participated in a serological survey performed in urban and rural areas of Manaus, Amazonas. DNA samples were extracted from the feces of triatomines used for xenodiagnosis, and the nontranscribed spacer of the mini-exon gene and the mitochondrial gene cytochrome oxidase subunit II (COII) were amplified by PCR and sequenced. Results Blood culture and xenodiagnosis were negative in 100% of samples; however, molecular techniques revealed that in 13 out of 36 (36%) fecal samples from xenodiagnosis, T. cruzi was characterized as the DTU TcI, and different haplotypes were identified within the same DTU. Conclusion The DTU TcI, which is mainly associated with acute cases of Chagas disease in the Amazon region, is also responsible for chronic infection in patients from a region in the State of Amazonas.https://doi.org/10.1186/1756-3305-7-267Amazon RegionTrypanosoma CruziSerological SurveyNontranscribed SpacerDiscrete Typing Unit |
| spellingShingle | Rosa Amélia Gonçalves Santana Laylah Kelre Costa Magalhães Laise Kelman Costa Magalhães Suzane Ribeiro Prestes Marcel Gonçalves Maciel George Allan Villarouco da Silva Wuelton Marcelo Monteiro Felipe Rocha de Brito Leila Inês de Aguiar Raposo Câmara Coelho João Marcos Barbosa-Ferreira Jorge Augusto Oliveira Guerra Henrique Silveira Maria das Graças Vale Barbosa Trypanosoma cruzi strain TcI is associated with chronic Chagas disease in the Brazilian Amazon Parasites & Vectors Amazon Region Trypanosoma Cruzi Serological Survey Nontranscribed Spacer Discrete Typing Unit |
| title | Trypanosoma cruzi strain TcI is associated with chronic Chagas disease in the Brazilian Amazon |
| title_full | Trypanosoma cruzi strain TcI is associated with chronic Chagas disease in the Brazilian Amazon |
| title_fullStr | Trypanosoma cruzi strain TcI is associated with chronic Chagas disease in the Brazilian Amazon |
| title_full_unstemmed | Trypanosoma cruzi strain TcI is associated with chronic Chagas disease in the Brazilian Amazon |
| title_short | Trypanosoma cruzi strain TcI is associated with chronic Chagas disease in the Brazilian Amazon |
| title_sort | trypanosoma cruzi strain tci is associated with chronic chagas disease in the brazilian amazon |
| topic | Amazon Region Trypanosoma Cruzi Serological Survey Nontranscribed Spacer Discrete Typing Unit |
| url | https://doi.org/10.1186/1756-3305-7-267 |
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