Introducing <i>Cyanodorina</i> gen. nov. and <i>Cyanodorina ovale</i> sp. nov. (Microcystaceae, Chroococcales), a Novel Coccoid Cyanobacterium Isolated from Caohai Lake in China Based on a Polyphasic Approach
The Chroococcales is one of the least studied cyanobacterial orders comprising the non-baeocyte-producing coccoids cyanobacteria with stacked and fasciculated thylakoids. During a survey of aquatic biodiversity in Caohai Lake in Guizhou Province, China, a coccoid-like cyanobacterium was isolated. It...
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MDPI AG
2023-02-01
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| author | Wei Chen Shuyin Li Yuanzhao Xu Ruozhen Geng Gaofei Song Peiming Ma |
| author_facet | Wei Chen Shuyin Li Yuanzhao Xu Ruozhen Geng Gaofei Song Peiming Ma |
| author_sort | Wei Chen |
| collection | DOAJ |
| description | The Chroococcales is one of the least studied cyanobacterial orders comprising the non-baeocyte-producing coccoids cyanobacteria with stacked and fasciculated thylakoids. During a survey of aquatic biodiversity in Caohai Lake in Guizhou Province, China, a coccoid-like cyanobacterium was isolated. It was characterized using a polyphasic approach, based on morphology, electron microscopy, and molecular phylogenetic analyses. This species’ colonies exhibited morphological similarity to those of <i>Microcystis</i> species but differed in their larger colony sizes and widely oval cells. The 16S rRNA gene sequence of this species had the maximum homology, corresponding to 93.10%, to that of the genus <i>Microcystis</i>. The results of 16S rRNA gene threshold value and 16S rRNA phylogenetic analyses confirmed that the studied species belongs to the family Microcystaceae but is phylogenetically distinct from the other species of Microcystaceae. Furthermore, The D1–D1′, Box–B helix, and V3 helix of the 16S–23S ITS region were also different from those previously described in Microcystaceae taxa. Combining the morphological, ecological, and molecular features of the coccoid-like cyanobacterium, we here propose the establishment of the <i>Cyanodorina</i> gen. nov. and the <i>Cyanodorina ovale</i> sp. nov. |
| first_indexed | 2024-03-11T06:41:06Z |
| format | Article |
| id | doaj.art-b0f18df7c5494305984b961495dd4d85 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-11T06:41:06Z |
| publishDate | 2023-02-01 |
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| spelling | doaj.art-b0f18df7c5494305984b961495dd4d852023-11-17T10:37:09ZengMDPI AGDiversity1424-28182023-02-0115332910.3390/d15030329Introducing <i>Cyanodorina</i> gen. nov. and <i>Cyanodorina ovale</i> sp. nov. (Microcystaceae, Chroococcales), a Novel Coccoid Cyanobacterium Isolated from Caohai Lake in China Based on a Polyphasic ApproachWei Chen0Shuyin Li1Yuanzhao Xu2Ruozhen Geng3Gaofei Song4Peiming Ma5Key Laboratory of Ecological Impacts of Hydraulic Projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources & Chinese Academy of Sciences, Wuhan 430079, ChinaYangtze River Basin Ecological Environment Monitoring and Scientific Research Center, Yangtze River Basin Ecological Environment Supervision and Administration Bureau, Ministry of Ecological Environment, Wuhan 430010, ChinaYangtze River Basin Ecological Environment Monitoring and Scientific Research Center, Yangtze River Basin Ecological Environment Supervision and Administration Bureau, Ministry of Ecological Environment, Wuhan 430010, ChinaCollege of Life and Environmental Sciences, Wenzhou University, Wenzhou 325035, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaKey Laboratory of Ecological Impacts of Hydraulic Projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources & Chinese Academy of Sciences, Wuhan 430079, ChinaThe Chroococcales is one of the least studied cyanobacterial orders comprising the non-baeocyte-producing coccoids cyanobacteria with stacked and fasciculated thylakoids. During a survey of aquatic biodiversity in Caohai Lake in Guizhou Province, China, a coccoid-like cyanobacterium was isolated. It was characterized using a polyphasic approach, based on morphology, electron microscopy, and molecular phylogenetic analyses. This species’ colonies exhibited morphological similarity to those of <i>Microcystis</i> species but differed in their larger colony sizes and widely oval cells. The 16S rRNA gene sequence of this species had the maximum homology, corresponding to 93.10%, to that of the genus <i>Microcystis</i>. The results of 16S rRNA gene threshold value and 16S rRNA phylogenetic analyses confirmed that the studied species belongs to the family Microcystaceae but is phylogenetically distinct from the other species of Microcystaceae. Furthermore, The D1–D1′, Box–B helix, and V3 helix of the 16S–23S ITS region were also different from those previously described in Microcystaceae taxa. Combining the morphological, ecological, and molecular features of the coccoid-like cyanobacterium, we here propose the establishment of the <i>Cyanodorina</i> gen. nov. and the <i>Cyanodorina ovale</i> sp. nov.https://www.mdpi.com/1424-2818/15/3/329coccoid cyanobacteriamorphologyphylogeny16S rRNA gene16S–23S ITS |
| spellingShingle | Wei Chen Shuyin Li Yuanzhao Xu Ruozhen Geng Gaofei Song Peiming Ma Introducing <i>Cyanodorina</i> gen. nov. and <i>Cyanodorina ovale</i> sp. nov. (Microcystaceae, Chroococcales), a Novel Coccoid Cyanobacterium Isolated from Caohai Lake in China Based on a Polyphasic Approach Diversity coccoid cyanobacteria morphology phylogeny 16S rRNA gene 16S–23S ITS |
| title | Introducing <i>Cyanodorina</i> gen. nov. and <i>Cyanodorina ovale</i> sp. nov. (Microcystaceae, Chroococcales), a Novel Coccoid Cyanobacterium Isolated from Caohai Lake in China Based on a Polyphasic Approach |
| title_full | Introducing <i>Cyanodorina</i> gen. nov. and <i>Cyanodorina ovale</i> sp. nov. (Microcystaceae, Chroococcales), a Novel Coccoid Cyanobacterium Isolated from Caohai Lake in China Based on a Polyphasic Approach |
| title_fullStr | Introducing <i>Cyanodorina</i> gen. nov. and <i>Cyanodorina ovale</i> sp. nov. (Microcystaceae, Chroococcales), a Novel Coccoid Cyanobacterium Isolated from Caohai Lake in China Based on a Polyphasic Approach |
| title_full_unstemmed | Introducing <i>Cyanodorina</i> gen. nov. and <i>Cyanodorina ovale</i> sp. nov. (Microcystaceae, Chroococcales), a Novel Coccoid Cyanobacterium Isolated from Caohai Lake in China Based on a Polyphasic Approach |
| title_short | Introducing <i>Cyanodorina</i> gen. nov. and <i>Cyanodorina ovale</i> sp. nov. (Microcystaceae, Chroococcales), a Novel Coccoid Cyanobacterium Isolated from Caohai Lake in China Based on a Polyphasic Approach |
| title_sort | introducing i cyanodorina i gen nov and i cyanodorina ovale i sp nov microcystaceae chroococcales a novel coccoid cyanobacterium isolated from caohai lake in china based on a polyphasic approach |
| topic | coccoid cyanobacteria morphology phylogeny 16S rRNA gene 16S–23S ITS |
| url | https://www.mdpi.com/1424-2818/15/3/329 |
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