A novel bacteriocin against multiple foodborne pathogens from Lacticaseibacillus rhamnosus isolated from juice ferments: ATF perfusion-based preparation of viable cells, characterization, antibacterial and antibiofilm activity
Foodborne pathogens and their biofilms pose a risk to human health through food chain. However, the bacteriocin resources combating this threat are still limited. Here, Lacticaseibacillus rhamnosus, one of the most used probiotics in food industry, was prepared on a large scale using alternating tan...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Current Research in Food Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665927123000527 |
| _version_ | 1827919347510673408 |
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| author | Shi-Yu Chen Rui-Si Yang Bai-Quan Ci Wei-Gang Xin Qi-Lin Zhang Lian-Bing Lin Feng Wang |
| author_facet | Shi-Yu Chen Rui-Si Yang Bai-Quan Ci Wei-Gang Xin Qi-Lin Zhang Lian-Bing Lin Feng Wang |
| author_sort | Shi-Yu Chen |
| collection | DOAJ |
| description | Foodborne pathogens and their biofilms pose a risk to human health through food chain. However, the bacteriocin resources combating this threat are still limited. Here, Lacticaseibacillus rhamnosus, one of the most used probiotics in food industry, was prepared on a large scale using alternating tangential flow (ATF) perfusion-based technology. Compared to the conventional fed-batch approach, ATF perfusion remarkably increased the viable cells of L. rhamnosus CLK 101 to 11.93 ± 0.14 log CFU/mL. Based on obtained viable cells, we purified and characterized a novel bacteriocin CLK_01 with a broad spectrum of activity against both Gram-positive and Gram-negative foodborne pathogens. LC-MS/MS analysis revealed that CLK_01 has a molecular mass of 701.49 Da and a hydrophobic amino acid composition of I–K–K–V-T-I. As a novel bacteriocin, CLK_01 showed high thermal stability and acid-base tolerance over 25–121 °C and pH 2–10. It significantly reduced cell viability of bacterial pathogens (p < 0.001), and strongly inhibited their biofilm formation. Scanning electron microscopy demonstrated deformation of pathogenic cells caused by CLK_01, leading to cytoplasmic content leakage and bacterial death. Summarily, we employed ATF perfusion to obtain viable L. rhamnosus, and presented that bacteriocin CLK_01 could serve as a promising biopreservative for controlling foodborne pathogenic bacteria and their biofilms. |
| first_indexed | 2024-03-13T03:57:14Z |
| format | Article |
| id | doaj.art-b0f1ab26828c4fb5b001562dc65f2443 |
| institution | Directory Open Access Journal |
| issn | 2665-9271 |
| language | English |
| last_indexed | 2024-03-13T03:57:14Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj.art-b0f1ab26828c4fb5b001562dc65f24432023-06-22T05:05:04ZengElsevierCurrent Research in Food Science2665-92712023-01-016100484A novel bacteriocin against multiple foodborne pathogens from Lacticaseibacillus rhamnosus isolated from juice ferments: ATF perfusion-based preparation of viable cells, characterization, antibacterial and antibiofilm activityShi-Yu Chen0Rui-Si Yang1Bai-Quan Ci2Wei-Gang Xin3Qi-Lin Zhang4Lian-Bing Lin5Feng Wang6Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China; Engineering Research Center for Replacement Technology of Feed Antibiotics of Yunnan College, Kunming, 650500, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China; Engineering Research Center for Replacement Technology of Feed Antibiotics of Yunnan College, Kunming, 650500, China; Corresponding author. Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan, Kunming, 650500, China.Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China; Engineering Research Center for Replacement Technology of Feed Antibiotics of Yunnan College, Kunming, 650500, China; Corresponding author. Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan, Kunming, 650500, China.Foodborne pathogens and their biofilms pose a risk to human health through food chain. However, the bacteriocin resources combating this threat are still limited. Here, Lacticaseibacillus rhamnosus, one of the most used probiotics in food industry, was prepared on a large scale using alternating tangential flow (ATF) perfusion-based technology. Compared to the conventional fed-batch approach, ATF perfusion remarkably increased the viable cells of L. rhamnosus CLK 101 to 11.93 ± 0.14 log CFU/mL. Based on obtained viable cells, we purified and characterized a novel bacteriocin CLK_01 with a broad spectrum of activity against both Gram-positive and Gram-negative foodborne pathogens. LC-MS/MS analysis revealed that CLK_01 has a molecular mass of 701.49 Da and a hydrophobic amino acid composition of I–K–K–V-T-I. As a novel bacteriocin, CLK_01 showed high thermal stability and acid-base tolerance over 25–121 °C and pH 2–10. It significantly reduced cell viability of bacterial pathogens (p < 0.001), and strongly inhibited their biofilm formation. Scanning electron microscopy demonstrated deformation of pathogenic cells caused by CLK_01, leading to cytoplasmic content leakage and bacterial death. Summarily, we employed ATF perfusion to obtain viable L. rhamnosus, and presented that bacteriocin CLK_01 could serve as a promising biopreservative for controlling foodborne pathogenic bacteria and their biofilms.http://www.sciencedirect.com/science/article/pii/S2665927123000527L. rhamnosusFoodborne pathogenBacteriocinAlternating tangential flowAntimicrobial activity |
| spellingShingle | Shi-Yu Chen Rui-Si Yang Bai-Quan Ci Wei-Gang Xin Qi-Lin Zhang Lian-Bing Lin Feng Wang A novel bacteriocin against multiple foodborne pathogens from Lacticaseibacillus rhamnosus isolated from juice ferments: ATF perfusion-based preparation of viable cells, characterization, antibacterial and antibiofilm activity Current Research in Food Science L. rhamnosus Foodborne pathogen Bacteriocin Alternating tangential flow Antimicrobial activity |
| title | A novel bacteriocin against multiple foodborne pathogens from Lacticaseibacillus rhamnosus isolated from juice ferments: ATF perfusion-based preparation of viable cells, characterization, antibacterial and antibiofilm activity |
| title_full | A novel bacteriocin against multiple foodborne pathogens from Lacticaseibacillus rhamnosus isolated from juice ferments: ATF perfusion-based preparation of viable cells, characterization, antibacterial and antibiofilm activity |
| title_fullStr | A novel bacteriocin against multiple foodborne pathogens from Lacticaseibacillus rhamnosus isolated from juice ferments: ATF perfusion-based preparation of viable cells, characterization, antibacterial and antibiofilm activity |
| title_full_unstemmed | A novel bacteriocin against multiple foodborne pathogens from Lacticaseibacillus rhamnosus isolated from juice ferments: ATF perfusion-based preparation of viable cells, characterization, antibacterial and antibiofilm activity |
| title_short | A novel bacteriocin against multiple foodborne pathogens from Lacticaseibacillus rhamnosus isolated from juice ferments: ATF perfusion-based preparation of viable cells, characterization, antibacterial and antibiofilm activity |
| title_sort | novel bacteriocin against multiple foodborne pathogens from lacticaseibacillus rhamnosus isolated from juice ferments atf perfusion based preparation of viable cells characterization antibacterial and antibiofilm activity |
| topic | L. rhamnosus Foodborne pathogen Bacteriocin Alternating tangential flow Antimicrobial activity |
| url | http://www.sciencedirect.com/science/article/pii/S2665927123000527 |
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