Screening of Pig-Derived Zearalenone-Degrading Bacteria through the Zearalenone Challenge Model, and Their Degradation Characteristics
Zearalenone (ZEN) is widely found in food and feed. Its cytotoxicity, reproductive toxicity, genetic toxicity, immunotoxicity and hepatorenal toxicity have serious impacts on human and animal health. In order to help animals avoid ZEN poisoning in feed, ZEN-degrading bacterial strains were screened...
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MDPI AG
2022-03-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/3/224 |
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| author | Xue Yang Feng Li Hangyi Ning Wei Zhang Dongyan Niu Zhuo Shi Sa Chai Anshan Shan |
| author_facet | Xue Yang Feng Li Hangyi Ning Wei Zhang Dongyan Niu Zhuo Shi Sa Chai Anshan Shan |
| author_sort | Xue Yang |
| collection | DOAJ |
| description | Zearalenone (ZEN) is widely found in food and feed. Its cytotoxicity, reproductive toxicity, genetic toxicity, immunotoxicity and hepatorenal toxicity have serious impacts on human and animal health. In order to help animals avoid ZEN poisoning in feed, ZEN-degrading bacterial strains were screened from fecal samples through a zearalenone challenge pig model, and their degradation characteristics were researched. Through the optimization of parameters such as the culture time, pH value, temperature, and strain concentration, the optimal conditions for the ZEN-degrading ability of these strains were preliminarily determined, and the active site of the ZEN degradation was explored. In this study, three strains (SY-3, SY-14, SY-20) with high ZEN degradation capacities were obtained. SY-3 was identified as <i>Proteus mirabilis</i>, and its main degrading component was the supernatant. SY-14 and SY-20 were identified as <i>Bacillus subtilis</i>. Their main degrading components were the intracellular fluid of SY-14, and the intracellular fluid and cell wall of SY-20. The above results showed that the ZEN challenge model was an effective way to screen ZEN-degrading bacteria. |
| first_indexed | 2024-03-09T12:22:04Z |
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| id | doaj.art-b0f1b7fc64f24b7ba808ffbdcbda9021 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T12:22:04Z |
| publishDate | 2022-03-01 |
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| series | Toxins |
| spelling | doaj.art-b0f1b7fc64f24b7ba808ffbdcbda90212023-11-30T22:39:48ZengMDPI AGToxins2072-66512022-03-0114322410.3390/toxins14030224Screening of Pig-Derived Zearalenone-Degrading Bacteria through the Zearalenone Challenge Model, and Their Degradation CharacteristicsXue Yang0Feng Li1Hangyi Ning2Wei Zhang3Dongyan Niu4Zhuo Shi5Sa Chai6Anshan Shan7Institute of Animal Nutrition, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaInstitute of Animal Nutrition, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaInstitute of Animal Nutrition, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaInstitute of Animal Nutrition, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaDepartment of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 4Z6, CanadaInstitute of Animal Nutrition, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaInstitute of Animal Nutrition, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaInstitute of Animal Nutrition, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaZearalenone (ZEN) is widely found in food and feed. Its cytotoxicity, reproductive toxicity, genetic toxicity, immunotoxicity and hepatorenal toxicity have serious impacts on human and animal health. In order to help animals avoid ZEN poisoning in feed, ZEN-degrading bacterial strains were screened from fecal samples through a zearalenone challenge pig model, and their degradation characteristics were researched. Through the optimization of parameters such as the culture time, pH value, temperature, and strain concentration, the optimal conditions for the ZEN-degrading ability of these strains were preliminarily determined, and the active site of the ZEN degradation was explored. In this study, three strains (SY-3, SY-14, SY-20) with high ZEN degradation capacities were obtained. SY-3 was identified as <i>Proteus mirabilis</i>, and its main degrading component was the supernatant. SY-14 and SY-20 were identified as <i>Bacillus subtilis</i>. Their main degrading components were the intracellular fluid of SY-14, and the intracellular fluid and cell wall of SY-20. The above results showed that the ZEN challenge model was an effective way to screen ZEN-degrading bacteria.https://www.mdpi.com/2072-6651/14/3/224zearalenone<i>Proteus mirabilis</i><i>Bacillus subtilis</i>pig-derived zearalenone-degrading strainszearalenone challenge pig model |
| spellingShingle | Xue Yang Feng Li Hangyi Ning Wei Zhang Dongyan Niu Zhuo Shi Sa Chai Anshan Shan Screening of Pig-Derived Zearalenone-Degrading Bacteria through the Zearalenone Challenge Model, and Their Degradation Characteristics Toxins zearalenone <i>Proteus mirabilis</i> <i>Bacillus subtilis</i> pig-derived zearalenone-degrading strains zearalenone challenge pig model |
| title | Screening of Pig-Derived Zearalenone-Degrading Bacteria through the Zearalenone Challenge Model, and Their Degradation Characteristics |
| title_full | Screening of Pig-Derived Zearalenone-Degrading Bacteria through the Zearalenone Challenge Model, and Their Degradation Characteristics |
| title_fullStr | Screening of Pig-Derived Zearalenone-Degrading Bacteria through the Zearalenone Challenge Model, and Their Degradation Characteristics |
| title_full_unstemmed | Screening of Pig-Derived Zearalenone-Degrading Bacteria through the Zearalenone Challenge Model, and Their Degradation Characteristics |
| title_short | Screening of Pig-Derived Zearalenone-Degrading Bacteria through the Zearalenone Challenge Model, and Their Degradation Characteristics |
| title_sort | screening of pig derived zearalenone degrading bacteria through the zearalenone challenge model and their degradation characteristics |
| topic | zearalenone <i>Proteus mirabilis</i> <i>Bacillus subtilis</i> pig-derived zearalenone-degrading strains zearalenone challenge pig model |
| url | https://www.mdpi.com/2072-6651/14/3/224 |
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