Wheat straw delignification by Djerkandera adusta (Willd.) P. Karst. 1879: The effect on enzymatic hydrolysis
The use of lignocellulosic materials in the production of biofuels and biochemicals holds a huge prospect since wood and agricultural residues represent the most abundant global source of renewable biomass. However, delignification is an inevitable step in lignocellulose pre-treatment re...
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| Format: | Article |
| Language: | English |
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Matica srpska
2023-01-01
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| Series: | Zbornik Matice Srpske za Prirodne Nauke |
| Subjects: | |
| Online Access: | https://doiserbia.nb.rs/img/doi/0352-4906/2023/0352-49062344073D.pdf |
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| author | Đokić Ivana Z. Knežević Aleksandar Z. Savković Željko D. Vukojević Jelena B. |
| author_facet | Đokić Ivana Z. Knežević Aleksandar Z. Savković Željko D. Vukojević Jelena B. |
| author_sort | Đokić Ivana Z. |
| collection | DOAJ |
| description | The use of lignocellulosic materials in the production of biofuels and
biochemicals holds a huge prospect since wood and agricultural residues
represent the most abundant global source of renewable biomass. However,
delignification is an inevitable step in lignocellulose pre-treatment
rendering the cellulose and hemicellulose more exposed to enzymatic
saccharification. The aim of this study was to assess the potential of
different Bjerkandera adusta strains to enhance the efficiency of enzymatic
saccharification of wheat straw after solid-state culturing. Three white-rot
fungal strains of Bjerkandera adusta (Willd.) P. Karst. 1879, (BEOFB1601,
BEOFB1602 and BEOFB1603) were used for partial delignification of wheat
straw during solid-state cultivation. Activity of ligninolytic enzymes were
measured spectrophotometrically while wheat straw residues were used for
determination of hemicelluloses, cellulose and lignin contents. Enzymatic
hydrolysis of pre-treated wheat straw was conducted using commercial
cellulase in loadings of 60 U g-1 of solid substrate. The content of
reducing sugars was measured calorimetrically using 1,4-dinitrosalycilic
acid. Enzymes predominantly responsible for lignin degradation by tested
fungal strains were peroxidases. The highest rate of lignin degradation was
noticed in samples pretreated with the strain BEOFB1601 (42.3 ± 3.7%). The
highest reducing sugars yield (8.6 ± 0.3 gGE L-1) was achieved after
enzymatic saccharification of samples pre-treated with the strain BEOFB1601,
as the most selective lignin degrader. The obtained results suggest that
fungal culturing as a biological pre-treatment method can be significantly
strain specific. A key mechanism which enhances convertibility of
carbohydrates is selective lignin degradation of the biomass. |
| first_indexed | 2024-03-09T00:07:26Z |
| format | Article |
| id | doaj.art-b0fc8f80ac0b460c91bb637b627e654d |
| institution | Directory Open Access Journal |
| issn | 0352-4906 2406-0828 |
| language | English |
| last_indexed | 2024-03-09T00:07:26Z |
| publishDate | 2023-01-01 |
| publisher | Matica srpska |
| record_format | Article |
| series | Zbornik Matice Srpske za Prirodne Nauke |
| spelling | doaj.art-b0fc8f80ac0b460c91bb637b627e654d2023-12-12T13:13:20ZengMatica srpskaZbornik Matice Srpske za Prirodne Nauke0352-49062406-08282023-01-012023144738310.2298/ZMSPN2344073D0352-49062344073DWheat straw delignification by Djerkandera adusta (Willd.) P. Karst. 1879: The effect on enzymatic hydrolysisĐokić Ivana Z.0Knežević Aleksandar Z.1https://orcid.org/0000-0002-2776-9675Savković Željko D.2https://orcid.org/0000-0002-6678-4958Vukojević Jelena B.3https://orcid.org/0000-0002-6396-9789University of Belgrade, Faculty of Biology, Belgrade, SerbiaUniversity of Belgrade, Faculty of Biology, Belgrade, SerbiaUniversity of Belgrade, Faculty of Biology, Belgrade, SerbiaUniversity of Belgrade, Faculty of Biology, Belgrade, SerbiaThe use of lignocellulosic materials in the production of biofuels and biochemicals holds a huge prospect since wood and agricultural residues represent the most abundant global source of renewable biomass. However, delignification is an inevitable step in lignocellulose pre-treatment rendering the cellulose and hemicellulose more exposed to enzymatic saccharification. The aim of this study was to assess the potential of different Bjerkandera adusta strains to enhance the efficiency of enzymatic saccharification of wheat straw after solid-state culturing. Three white-rot fungal strains of Bjerkandera adusta (Willd.) P. Karst. 1879, (BEOFB1601, BEOFB1602 and BEOFB1603) were used for partial delignification of wheat straw during solid-state cultivation. Activity of ligninolytic enzymes were measured spectrophotometrically while wheat straw residues were used for determination of hemicelluloses, cellulose and lignin contents. Enzymatic hydrolysis of pre-treated wheat straw was conducted using commercial cellulase in loadings of 60 U g-1 of solid substrate. The content of reducing sugars was measured calorimetrically using 1,4-dinitrosalycilic acid. Enzymes predominantly responsible for lignin degradation by tested fungal strains were peroxidases. The highest rate of lignin degradation was noticed in samples pretreated with the strain BEOFB1601 (42.3 ± 3.7%). The highest reducing sugars yield (8.6 ± 0.3 gGE L-1) was achieved after enzymatic saccharification of samples pre-treated with the strain BEOFB1601, as the most selective lignin degrader. The obtained results suggest that fungal culturing as a biological pre-treatment method can be significantly strain specific. A key mechanism which enhances convertibility of carbohydrates is selective lignin degradation of the biomass.https://doiserbia.nb.rs/img/doi/0352-4906/2023/0352-49062344073D.pdfbjerkandera adustaligninolytic enzymeswheat strawfungal pretreatmentsaccharification |
| spellingShingle | Đokić Ivana Z. Knežević Aleksandar Z. Savković Željko D. Vukojević Jelena B. Wheat straw delignification by Djerkandera adusta (Willd.) P. Karst. 1879: The effect on enzymatic hydrolysis Zbornik Matice Srpske za Prirodne Nauke bjerkandera adusta ligninolytic enzymes wheat straw fungal pretreatment saccharification |
| title | Wheat straw delignification by Djerkandera adusta (Willd.) P. Karst. 1879: The effect on enzymatic hydrolysis |
| title_full | Wheat straw delignification by Djerkandera adusta (Willd.) P. Karst. 1879: The effect on enzymatic hydrolysis |
| title_fullStr | Wheat straw delignification by Djerkandera adusta (Willd.) P. Karst. 1879: The effect on enzymatic hydrolysis |
| title_full_unstemmed | Wheat straw delignification by Djerkandera adusta (Willd.) P. Karst. 1879: The effect on enzymatic hydrolysis |
| title_short | Wheat straw delignification by Djerkandera adusta (Willd.) P. Karst. 1879: The effect on enzymatic hydrolysis |
| title_sort | wheat straw delignification by djerkandera adusta willd p karst 1879 the effect on enzymatic hydrolysis |
| topic | bjerkandera adusta ligninolytic enzymes wheat straw fungal pretreatment saccharification |
| url | https://doiserbia.nb.rs/img/doi/0352-4906/2023/0352-49062344073D.pdf |
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