Phenotypic microarrays suggest Escherichia coli ST131 is not a metabolically distinct lineage of extra-intestinal pathogenic E. coli.

Extraintestinal pathogenic E. coli (ExPEC) are the major aetiological agent of urinary tract infections (UTIs) in humans. The emergence of the CTX-M producing clone E. coli ST131 represents a major challenge to public health worldwide. A recent study on the metabolic potential of E. coli isolates de...

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Main Authors: Abdulaziz Alqasim, Richard Emes, Gemma Clark, Jane Newcombe, Roberto La Ragione, Alan McNally
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24516644/pdf/?tool=EBI
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author Abdulaziz Alqasim
Richard Emes
Gemma Clark
Jane Newcombe
Roberto La Ragione
Alan McNally
author_facet Abdulaziz Alqasim
Richard Emes
Gemma Clark
Jane Newcombe
Roberto La Ragione
Alan McNally
author_sort Abdulaziz Alqasim
collection DOAJ
description Extraintestinal pathogenic E. coli (ExPEC) are the major aetiological agent of urinary tract infections (UTIs) in humans. The emergence of the CTX-M producing clone E. coli ST131 represents a major challenge to public health worldwide. A recent study on the metabolic potential of E. coli isolates demonstrated an association between the E. coli ST131 clone and enhanced utilisation of a panel of metabolic substrates. The studies presented here investigated the metabolic potential of ST131 and other major ExPEC ST isolates using 120 API test reagents and found that ST131 isolates demonstrated a lower metabolic activity for 5 of 120 biochemical tests in comparison to non-ST131 ExPEC isolates. Furthermore, comparative phenotypic microarray analysis showed a lack of specific metabolic profile for ST131 isolates countering the suggestion that these bacteria are metabolically fitter and therefore more successful human pathogens.
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spelling doaj.art-b0fd5dc1627b44d38dc18a0c4ecb49322022-12-21T21:43:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8837410.1371/journal.pone.0088374Phenotypic microarrays suggest Escherichia coli ST131 is not a metabolically distinct lineage of extra-intestinal pathogenic E. coli.Abdulaziz AlqasimRichard EmesGemma ClarkJane NewcombeRoberto La RagioneAlan McNallyExtraintestinal pathogenic E. coli (ExPEC) are the major aetiological agent of urinary tract infections (UTIs) in humans. The emergence of the CTX-M producing clone E. coli ST131 represents a major challenge to public health worldwide. A recent study on the metabolic potential of E. coli isolates demonstrated an association between the E. coli ST131 clone and enhanced utilisation of a panel of metabolic substrates. The studies presented here investigated the metabolic potential of ST131 and other major ExPEC ST isolates using 120 API test reagents and found that ST131 isolates demonstrated a lower metabolic activity for 5 of 120 biochemical tests in comparison to non-ST131 ExPEC isolates. Furthermore, comparative phenotypic microarray analysis showed a lack of specific metabolic profile for ST131 isolates countering the suggestion that these bacteria are metabolically fitter and therefore more successful human pathogens.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24516644/pdf/?tool=EBI
spellingShingle Abdulaziz Alqasim
Richard Emes
Gemma Clark
Jane Newcombe
Roberto La Ragione
Alan McNally
Phenotypic microarrays suggest Escherichia coli ST131 is not a metabolically distinct lineage of extra-intestinal pathogenic E. coli.
PLoS ONE
title Phenotypic microarrays suggest Escherichia coli ST131 is not a metabolically distinct lineage of extra-intestinal pathogenic E. coli.
title_full Phenotypic microarrays suggest Escherichia coli ST131 is not a metabolically distinct lineage of extra-intestinal pathogenic E. coli.
title_fullStr Phenotypic microarrays suggest Escherichia coli ST131 is not a metabolically distinct lineage of extra-intestinal pathogenic E. coli.
title_full_unstemmed Phenotypic microarrays suggest Escherichia coli ST131 is not a metabolically distinct lineage of extra-intestinal pathogenic E. coli.
title_short Phenotypic microarrays suggest Escherichia coli ST131 is not a metabolically distinct lineage of extra-intestinal pathogenic E. coli.
title_sort phenotypic microarrays suggest escherichia coli st131 is not a metabolically distinct lineage of extra intestinal pathogenic e coli
url https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24516644/pdf/?tool=EBI
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