MicroRNA-21 (miR-21) regulates cellular proliferation, invasion, migration, and apoptosis by targeting PTEN, RECK and Bcl-2 in lung squamous carcinoma, Gejiu City, China.
<h4>Background</h4>In South China (Gejiu City, Yunnan Province), lung cancer incidence and associated mortality rate is the most prevalent and observed forms of cancer. Lung cancer in this area is called Gejiu squamous cell lung carcinoma (GSQCLC). Research has demonstrated that overexpr...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25084400/pdf/?tool=EBI |
| _version_ | 1819320590083817472 |
|---|---|
| author | Long-Feng Xu Zhi-Ping Wu Yan Chen Qi-Shun Zhu Shoeleh Hamidi Roya Navab |
| author_facet | Long-Feng Xu Zhi-Ping Wu Yan Chen Qi-Shun Zhu Shoeleh Hamidi Roya Navab |
| author_sort | Long-Feng Xu |
| collection | DOAJ |
| description | <h4>Background</h4>In South China (Gejiu City, Yunnan Province), lung cancer incidence and associated mortality rate is the most prevalent and observed forms of cancer. Lung cancer in this area is called Gejiu squamous cell lung carcinoma (GSQCLC). Research has demonstrated that overexpression of miR-21 occurs in many cancers. However, the unique relationship between miR-21 and its target genes in GSQCLC has never been investigated. The molecular mechanism involved in GSQCLC must be compared to other non-small cell lung cancers in order to establish a relation and identify potential therapeutic targets.<h4>Methodology/principal findings</h4>In the current study, we initially found overexpression of miR-21 occurring in non-small cell lung cancer (NSCLC) cell lines when compared to the immortalized lung epithelial cell line BEAS-2B. We also demonstrated that high expression of miR-21 could increase tumor cell proliferation, invasion, viability, and migration in GSQCLC cell line (YTMLC-90) and NSCLC cell line (NCI-H157). Additionally, our results revealed that miR-21 could suppress YTMLC-90 and NCI-H157 cell apoptosis through arresting cell-cycle at G2/M phase. Furthermore, we demonstrated that PTEN, RECK and Bcl-2 are common target genes of miR-21 in NSCLC. Finally, our studies showed that down-regulation of miR-21 could lead to a significant increase in PTEN and RECK and decrease in Bcl-2 at the mRNA and protein level in YTMLC-90 and NCI-H157 cell lines. However, we have not observed any remarkable difference in the levels of miR-21 and its targets in YTMLC-90 cells when compared with NCI-H157 cells.<h4>Conclusions/significance</h4>miR-21 simultaneously regulates multiple programs that enhance cell proliferation, apoptosis and tumor invasiveness by targeting PTEN, RECK and Bcl-2 in GSQCLC. Our results demonstrated that miR-21 may play a vital role in tumorigenesis and progression of lung squamous cell carcinoma and suppression of miR-21 may be a novel approach for the treatment of lung squamous cell carcinoma. |
| first_indexed | 2024-12-24T11:21:57Z |
| format | Article |
| id | doaj.art-b0ffc815d4f6449c8ac03911eec706da |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-24T11:21:57Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b0ffc815d4f6449c8ac03911eec706da2022-12-21T16:58:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10369810.1371/journal.pone.0103698MicroRNA-21 (miR-21) regulates cellular proliferation, invasion, migration, and apoptosis by targeting PTEN, RECK and Bcl-2 in lung squamous carcinoma, Gejiu City, China.Long-Feng XuZhi-Ping WuYan ChenQi-Shun ZhuShoeleh HamidiRoya Navab<h4>Background</h4>In South China (Gejiu City, Yunnan Province), lung cancer incidence and associated mortality rate is the most prevalent and observed forms of cancer. Lung cancer in this area is called Gejiu squamous cell lung carcinoma (GSQCLC). Research has demonstrated that overexpression of miR-21 occurs in many cancers. However, the unique relationship between miR-21 and its target genes in GSQCLC has never been investigated. The molecular mechanism involved in GSQCLC must be compared to other non-small cell lung cancers in order to establish a relation and identify potential therapeutic targets.<h4>Methodology/principal findings</h4>In the current study, we initially found overexpression of miR-21 occurring in non-small cell lung cancer (NSCLC) cell lines when compared to the immortalized lung epithelial cell line BEAS-2B. We also demonstrated that high expression of miR-21 could increase tumor cell proliferation, invasion, viability, and migration in GSQCLC cell line (YTMLC-90) and NSCLC cell line (NCI-H157). Additionally, our results revealed that miR-21 could suppress YTMLC-90 and NCI-H157 cell apoptosis through arresting cell-cycle at G2/M phase. Furthermore, we demonstrated that PTEN, RECK and Bcl-2 are common target genes of miR-21 in NSCLC. Finally, our studies showed that down-regulation of miR-21 could lead to a significant increase in PTEN and RECK and decrease in Bcl-2 at the mRNA and protein level in YTMLC-90 and NCI-H157 cell lines. However, we have not observed any remarkable difference in the levels of miR-21 and its targets in YTMLC-90 cells when compared with NCI-H157 cells.<h4>Conclusions/significance</h4>miR-21 simultaneously regulates multiple programs that enhance cell proliferation, apoptosis and tumor invasiveness by targeting PTEN, RECK and Bcl-2 in GSQCLC. Our results demonstrated that miR-21 may play a vital role in tumorigenesis and progression of lung squamous cell carcinoma and suppression of miR-21 may be a novel approach for the treatment of lung squamous cell carcinoma.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25084400/pdf/?tool=EBI |
| spellingShingle | Long-Feng Xu Zhi-Ping Wu Yan Chen Qi-Shun Zhu Shoeleh Hamidi Roya Navab MicroRNA-21 (miR-21) regulates cellular proliferation, invasion, migration, and apoptosis by targeting PTEN, RECK and Bcl-2 in lung squamous carcinoma, Gejiu City, China. PLoS ONE |
| title | MicroRNA-21 (miR-21) regulates cellular proliferation, invasion, migration, and apoptosis by targeting PTEN, RECK and Bcl-2 in lung squamous carcinoma, Gejiu City, China. |
| title_full | MicroRNA-21 (miR-21) regulates cellular proliferation, invasion, migration, and apoptosis by targeting PTEN, RECK and Bcl-2 in lung squamous carcinoma, Gejiu City, China. |
| title_fullStr | MicroRNA-21 (miR-21) regulates cellular proliferation, invasion, migration, and apoptosis by targeting PTEN, RECK and Bcl-2 in lung squamous carcinoma, Gejiu City, China. |
| title_full_unstemmed | MicroRNA-21 (miR-21) regulates cellular proliferation, invasion, migration, and apoptosis by targeting PTEN, RECK and Bcl-2 in lung squamous carcinoma, Gejiu City, China. |
| title_short | MicroRNA-21 (miR-21) regulates cellular proliferation, invasion, migration, and apoptosis by targeting PTEN, RECK and Bcl-2 in lung squamous carcinoma, Gejiu City, China. |
| title_sort | microrna 21 mir 21 regulates cellular proliferation invasion migration and apoptosis by targeting pten reck and bcl 2 in lung squamous carcinoma gejiu city china |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25084400/pdf/?tool=EBI |
| work_keys_str_mv | AT longfengxu microrna21mir21regulatescellularproliferationinvasionmigrationandapoptosisbytargetingptenreckandbcl2inlungsquamouscarcinomagejiucitychina AT zhipingwu microrna21mir21regulatescellularproliferationinvasionmigrationandapoptosisbytargetingptenreckandbcl2inlungsquamouscarcinomagejiucitychina AT yanchen microrna21mir21regulatescellularproliferationinvasionmigrationandapoptosisbytargetingptenreckandbcl2inlungsquamouscarcinomagejiucitychina AT qishunzhu microrna21mir21regulatescellularproliferationinvasionmigrationandapoptosisbytargetingptenreckandbcl2inlungsquamouscarcinomagejiucitychina AT shoelehhamidi microrna21mir21regulatescellularproliferationinvasionmigrationandapoptosisbytargetingptenreckandbcl2inlungsquamouscarcinomagejiucitychina AT royanavab microrna21mir21regulatescellularproliferationinvasionmigrationandapoptosisbytargetingptenreckandbcl2inlungsquamouscarcinomagejiucitychina |