Elevated expression of Dickkopf-1 increases the sensitivity of human glioma cell line SHG<sub>44 </sub>to BCNU
<p>Abstract</p> <p>Background</p> <p>Studies have shown that Dickkopf-1 (DKK-1) is involved in tumorigenesis. Recently, we found that 9 out of 12 human glioma cell lines had high level of DKK-1 protein while the other 3 had very low or non-detectable level of DKK-1. The...
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| Format: | Article |
| Language: | English |
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BMC
2010-10-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
| Online Access: | http://www.jeccr.com/content/29/1/131 |
| _version_ | 1819027843123773440 |
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| author | Xu Qinian Li Wenshuai Zhou Youxin Huang Yulun |
| author_facet | Xu Qinian Li Wenshuai Zhou Youxin Huang Yulun |
| author_sort | Xu Qinian |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Studies have shown that Dickkopf-1 (DKK-1) is involved in tumorigenesis. Recently, we found that 9 out of 12 human glioma cell lines had high level of DKK-1 protein while the other 3 had very low or non-detectable level of DKK-1. The aim of this study is to further examine the function of DKK-1 in glioma cells.</p> <p>Materials and methods</p> <p>The glioma cell line SHG<sub>44 </sub>was obtained from a patient with grade II-III astrocytoma. SHG<sub>44 </sub>cells were transfected with a human DKK-1 gene. Transfection of the empty vector pcDNA3.1 was used as negative control. Sensitivity to BCNU was measured by Annexin-V staining. Expression of bax, bcl-2 and caspase-3 of three groups was determined by immunohistochemistry.</p> <p>Results</p> <p>The tranfection was confirmed by PCR, RT-PCR and Western blot. More apoptotic cell death was observed in the DKK-1 transfected cells, comparing to the non-transfected cells, or cells with empty vector. The expression of bax and caspase-3 of the SHG<sub>44 </sub>-DDK-1 increased, whereas the expression of bcl-2 decreased</p> <p>Conclusion</p> <p>Our results indicated that DKK-1 has a pro-apoptotic function of in glioma.</p> |
| first_indexed | 2024-12-21T05:48:54Z |
| format | Article |
| id | doaj.art-b101e14208db4fee96315187889028fe |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-12-21T05:48:54Z |
| publishDate | 2010-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-b101e14208db4fee96315187889028fe2022-12-21T19:14:03ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662010-10-0129113110.1186/1756-9966-29-131Elevated expression of Dickkopf-1 increases the sensitivity of human glioma cell line SHG<sub>44 </sub>to BCNUXu QinianLi WenshuaiZhou YouxinHuang Yulun<p>Abstract</p> <p>Background</p> <p>Studies have shown that Dickkopf-1 (DKK-1) is involved in tumorigenesis. Recently, we found that 9 out of 12 human glioma cell lines had high level of DKK-1 protein while the other 3 had very low or non-detectable level of DKK-1. The aim of this study is to further examine the function of DKK-1 in glioma cells.</p> <p>Materials and methods</p> <p>The glioma cell line SHG<sub>44 </sub>was obtained from a patient with grade II-III astrocytoma. SHG<sub>44 </sub>cells were transfected with a human DKK-1 gene. Transfection of the empty vector pcDNA3.1 was used as negative control. Sensitivity to BCNU was measured by Annexin-V staining. Expression of bax, bcl-2 and caspase-3 of three groups was determined by immunohistochemistry.</p> <p>Results</p> <p>The tranfection was confirmed by PCR, RT-PCR and Western blot. More apoptotic cell death was observed in the DKK-1 transfected cells, comparing to the non-transfected cells, or cells with empty vector. The expression of bax and caspase-3 of the SHG<sub>44 </sub>-DDK-1 increased, whereas the expression of bcl-2 decreased</p> <p>Conclusion</p> <p>Our results indicated that DKK-1 has a pro-apoptotic function of in glioma.</p>http://www.jeccr.com/content/29/1/131 |
| spellingShingle | Xu Qinian Li Wenshuai Zhou Youxin Huang Yulun Elevated expression of Dickkopf-1 increases the sensitivity of human glioma cell line SHG<sub>44 </sub>to BCNU Journal of Experimental & Clinical Cancer Research |
| title | Elevated expression of Dickkopf-1 increases the sensitivity of human glioma cell line SHG<sub>44 </sub>to BCNU |
| title_full | Elevated expression of Dickkopf-1 increases the sensitivity of human glioma cell line SHG<sub>44 </sub>to BCNU |
| title_fullStr | Elevated expression of Dickkopf-1 increases the sensitivity of human glioma cell line SHG<sub>44 </sub>to BCNU |
| title_full_unstemmed | Elevated expression of Dickkopf-1 increases the sensitivity of human glioma cell line SHG<sub>44 </sub>to BCNU |
| title_short | Elevated expression of Dickkopf-1 increases the sensitivity of human glioma cell line SHG<sub>44 </sub>to BCNU |
| title_sort | elevated expression of dickkopf 1 increases the sensitivity of human glioma cell line shg sub 44 sub to bcnu |
| url | http://www.jeccr.com/content/29/1/131 |
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