Detection of Y Chromosome Microdeletions and Hormonal Profile Analysis of Infertile Men undergoing Assisted Reproductive Technologies
Background Y chromosome deletions (YCDs) in azoospermia factor (AZF) region are associated with ab- normal spermatogenesis and may lead to azoospermia or severe oligozoospermia. Assisted reproductive tech- nologies (ART) by intracytoplasmic sperm injection (ICSI) and testicular sperm extraction (...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2018-07-01
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| Series: | International Journal of Fertility and Sterility |
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| Online Access: | http://www.ijfs.ir/article_45487_a8b0af5a844056de687e12246b7f0f2d.pdf |
| _version_ | 1818115188025458688 |
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| author | Ardeshir Bahmanimehr Shahryar Zeighami Bahia Namavar Jahromi Mohammad Ebrahim Parsanezhad Maryam Davari Somayeh Montazeri Najmeh Moein Vaziri Afsoon Zarei |
| author_facet | Ardeshir Bahmanimehr Shahryar Zeighami Bahia Namavar Jahromi Mohammad Ebrahim Parsanezhad Maryam Davari Somayeh Montazeri Najmeh Moein Vaziri Afsoon Zarei |
| author_sort | Ardeshir Bahmanimehr |
| collection | DOAJ |
| description | Background Y chromosome deletions (YCDs) in azoospermia factor (AZF) region are associated with ab- normal spermatogenesis and may lead to azoospermia or severe oligozoospermia. Assisted reproductive tech- nologies (ART) by intracytoplasmic sperm injection (ICSI) and testicular sperm extraction (TESE) are com- monly required for infertility management of patients carrying YCDs. The aim of this study was to estimate the frequency of YCDs, to find the most frequent variant in infertile men candidate for ART and to compare YCD distribution with a control fertile group. The semen parameters, hormonal profiles and ART outcomes of the infertile group were studied. Materials and Methods This case-control study consisted of 97 oligozoospermic or non-obstructive azoospermic (NOA) infertile men, who had undergone ART, as the case group and 100 fertile men as the control group. DNA samples were extracted from blood samples taken from all 197 participants and YCDs were identified by multiplex polymerase chain reaction (PCR) of eight known sequence-tagged sites. The chi-square test was used to compare the mean values of hormone and sperm parameters between the two groups. P < 0.05 was considered statistically significant. Results No YCD was detected in the control group. However, 20 out of 97 (20.6%) infertile men had a YCD. AZFc, AZFbc and AZFabc deletions were detected in 15 (75%), four (20%) and one (5%) YCD-positive patients. No fer- tilization or clinical pregnancy was seen following ICSI in this sub-group with YCD. The mean level of FSH was significantly higher in the group with YCD (28.45 ± 22.2 vs. 4.8 ± 3.17 and 10.83 ± 7.23 in YCD-negative patients with and without clinical pregnancy respectively). Conclusion YCD is frequent among NOA men and YCD screening before ART and patient counseling is thus strongly recommended. |
| first_indexed | 2024-12-11T04:02:39Z |
| format | Article |
| id | doaj.art-b104b964c3904dcface1c02af0933f49 |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-12-11T04:02:39Z |
| publishDate | 2018-07-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-b104b964c3904dcface1c02af0933f492022-12-22T01:21:36ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782018-07-0112217317710.22074/ijfs.2018.524445487Detection of Y Chromosome Microdeletions and Hormonal Profile Analysis of Infertile Men undergoing Assisted Reproductive TechnologiesArdeshir Bahmanimehr0Shahryar Zeighami1Bahia Namavar Jahromi2Mohammad Ebrahim Parsanezhad3Maryam Davari4Somayeh Montazeri5Najmeh Moein Vaziri6Afsoon Zarei7Thalassemia and Hemophilia Genetic, PND Research Center, Dastgheib Hospital, Shiraz University of Medical Science, Shiraz, IranInfertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;Depatment of Urology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranInfertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;4Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranInfertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;4Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran4Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;5IVF Center, Ghadir Mother and Child Hospital, Shiraz University of Medical Sciences, Shiraz, IranThalassemia and Hemophilia Genetic, PND Research Center, Dastgheib Hospital, Shiraz University of Medical Science, Shiraz, IranInfertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;4Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranInfertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;4Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranBackground Y chromosome deletions (YCDs) in azoospermia factor (AZF) region are associated with ab- normal spermatogenesis and may lead to azoospermia or severe oligozoospermia. Assisted reproductive tech- nologies (ART) by intracytoplasmic sperm injection (ICSI) and testicular sperm extraction (TESE) are com- monly required for infertility management of patients carrying YCDs. The aim of this study was to estimate the frequency of YCDs, to find the most frequent variant in infertile men candidate for ART and to compare YCD distribution with a control fertile group. The semen parameters, hormonal profiles and ART outcomes of the infertile group were studied. Materials and Methods This case-control study consisted of 97 oligozoospermic or non-obstructive azoospermic (NOA) infertile men, who had undergone ART, as the case group and 100 fertile men as the control group. DNA samples were extracted from blood samples taken from all 197 participants and YCDs were identified by multiplex polymerase chain reaction (PCR) of eight known sequence-tagged sites. The chi-square test was used to compare the mean values of hormone and sperm parameters between the two groups. P < 0.05 was considered statistically significant. Results No YCD was detected in the control group. However, 20 out of 97 (20.6%) infertile men had a YCD. AZFc, AZFbc and AZFabc deletions were detected in 15 (75%), four (20%) and one (5%) YCD-positive patients. No fer- tilization or clinical pregnancy was seen following ICSI in this sub-group with YCD. The mean level of FSH was significantly higher in the group with YCD (28.45 ± 22.2 vs. 4.8 ± 3.17 and 10.83 ± 7.23 in YCD-negative patients with and without clinical pregnancy respectively). Conclusion YCD is frequent among NOA men and YCD screening before ART and patient counseling is thus strongly recommended.http://www.ijfs.ir/article_45487_a8b0af5a844056de687e12246b7f0f2d.pdfassisted reproductive technologiesnonobstructive azoospermiay chromosome deletion |
| spellingShingle | Ardeshir Bahmanimehr Shahryar Zeighami Bahia Namavar Jahromi Mohammad Ebrahim Parsanezhad Maryam Davari Somayeh Montazeri Najmeh Moein Vaziri Afsoon Zarei Detection of Y Chromosome Microdeletions and Hormonal Profile Analysis of Infertile Men undergoing Assisted Reproductive Technologies International Journal of Fertility and Sterility assisted reproductive technologies non obstructive azoospermia y chromosome deletion |
| title | Detection of Y Chromosome Microdeletions and Hormonal Profile Analysis of Infertile Men undergoing Assisted Reproductive Technologies |
| title_full | Detection of Y Chromosome Microdeletions and Hormonal Profile Analysis of Infertile Men undergoing Assisted Reproductive Technologies |
| title_fullStr | Detection of Y Chromosome Microdeletions and Hormonal Profile Analysis of Infertile Men undergoing Assisted Reproductive Technologies |
| title_full_unstemmed | Detection of Y Chromosome Microdeletions and Hormonal Profile Analysis of Infertile Men undergoing Assisted Reproductive Technologies |
| title_short | Detection of Y Chromosome Microdeletions and Hormonal Profile Analysis of Infertile Men undergoing Assisted Reproductive Technologies |
| title_sort | detection of y chromosome microdeletions and hormonal profile analysis of infertile men undergoing assisted reproductive technologies |
| topic | assisted reproductive technologies non obstructive azoospermia y chromosome deletion |
| url | http://www.ijfs.ir/article_45487_a8b0af5a844056de687e12246b7f0f2d.pdf |
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