TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 Expression
TWIK-1 is the first identified member of the two-pore domain potassium (K2P) channels that are involved in neuronal excitability and astrocytic passive conductance in the brain. Despite the physiological roles of TWIK-1, there is still a lack of information on the basic expression patterns of TWIK-1...
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MDPI AG
2021-10-01
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| author | Osung Kwon Hayoung Yang Seung-Chan Kim Juhyun Kim Jaewon Sim Jiyoun Lee Eun-Mi Hwang Sungbo Shim Jae-Yong Park |
| author_facet | Osung Kwon Hayoung Yang Seung-Chan Kim Juhyun Kim Jaewon Sim Jiyoun Lee Eun-Mi Hwang Sungbo Shim Jae-Yong Park |
| author_sort | Osung Kwon |
| collection | DOAJ |
| description | TWIK-1 is the first identified member of the two-pore domain potassium (K2P) channels that are involved in neuronal excitability and astrocytic passive conductance in the brain. Despite the physiological roles of TWIK-1, there is still a lack of information on the basic expression patterns of TWIK-1 proteins in the brain. Here, using a modified bacterial artificial chromosome (BAC), we generated a transgenic mouse (Tg mouse) line expressing green fluorescent protein (GFP) under the control of the TWIK-1 promoter (TWIK-1 BAC-GFP Tg mice). We confirmed that nearly all GFP-producing cells co-expressed endogenous TWIK-1 in the brain of TWIK-1 BAC-GFP Tg mice. GFP signals were highly expressed in various brain areas, including the dentate gyrus (DG), lateral entorhinal cortex (LEC), and cerebellum (Cb). In addition, we found that GFP signals were highly expressed in immature granule cells in the DG. Finally, our TWIK-1 BAC-GFP Tg mice mimic the upregulation of TWIK-1 mRNA expression in the hippocampus following the injection of kainic acid (KA). Our data clearly showed that TWIK-1 BAC-GFP Tg mice are a useful animal model for studying the mechanisms regulating TWIK-1 gene expression and the physiological roles of TWIK-1 channels in the brain. |
| first_indexed | 2024-03-10T06:39:48Z |
| format | Article |
| id | doaj.art-b105be517146448ba9e402eda0d24cd3 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T06:39:48Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
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| series | Cells |
| spelling | doaj.art-b105be517146448ba9e402eda0d24cd32023-11-22T17:48:35ZengMDPI AGCells2073-44092021-10-011010275110.3390/cells10102751TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 ExpressionOsung Kwon0Hayoung Yang1Seung-Chan Kim2Juhyun Kim3Jaewon Sim4Jiyoun Lee5Eun-Mi Hwang6Sungbo Shim7Jae-Yong Park8Department of Integrated Biomedical and Life Science, Graduate School, Jiyoun Lee Korea University, Seoul 02841, KoreaDepartment of Biochemistry, Chungbuk National University, Cheongju 28644, KoreaDepartment of Integrated Biomedical and Life Science, Graduate School, Jiyoun Lee Korea University, Seoul 02841, KoreaDepartment of Integrated Biomedical and Life Science, Graduate School, Jiyoun Lee Korea University, Seoul 02841, KoreaDepartment of Integrated Biomedical and Life Science, Graduate School, Jiyoun Lee Korea University, Seoul 02841, KoreaDepartment of Integrated Biomedical and Life Science, Graduate School, Jiyoun Lee Korea University, Seoul 02841, KoreaCenter for Functional Connectomics, Korea Institute of Science and Technology (KIST), Seoul 02792, KoreaDepartment of Biochemistry, Chungbuk National University, Cheongju 28644, KoreaDepartment of Integrated Biomedical and Life Science, Graduate School, Jiyoun Lee Korea University, Seoul 02841, KoreaTWIK-1 is the first identified member of the two-pore domain potassium (K2P) channels that are involved in neuronal excitability and astrocytic passive conductance in the brain. Despite the physiological roles of TWIK-1, there is still a lack of information on the basic expression patterns of TWIK-1 proteins in the brain. Here, using a modified bacterial artificial chromosome (BAC), we generated a transgenic mouse (Tg mouse) line expressing green fluorescent protein (GFP) under the control of the TWIK-1 promoter (TWIK-1 BAC-GFP Tg mice). We confirmed that nearly all GFP-producing cells co-expressed endogenous TWIK-1 in the brain of TWIK-1 BAC-GFP Tg mice. GFP signals were highly expressed in various brain areas, including the dentate gyrus (DG), lateral entorhinal cortex (LEC), and cerebellum (Cb). In addition, we found that GFP signals were highly expressed in immature granule cells in the DG. Finally, our TWIK-1 BAC-GFP Tg mice mimic the upregulation of TWIK-1 mRNA expression in the hippocampus following the injection of kainic acid (KA). Our data clearly showed that TWIK-1 BAC-GFP Tg mice are a useful animal model for studying the mechanisms regulating TWIK-1 gene expression and the physiological roles of TWIK-1 channels in the brain.https://www.mdpi.com/2073-4409/10/10/2751TWIK-1bacterial artificial chromosome transgenic mousedentate gyruslateral entorhinal cortexcerebellum |
| spellingShingle | Osung Kwon Hayoung Yang Seung-Chan Kim Juhyun Kim Jaewon Sim Jiyoun Lee Eun-Mi Hwang Sungbo Shim Jae-Yong Park TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 Expression Cells TWIK-1 bacterial artificial chromosome transgenic mouse dentate gyrus lateral entorhinal cortex cerebellum |
| title | TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 Expression |
| title_full | TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 Expression |
| title_fullStr | TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 Expression |
| title_full_unstemmed | TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 Expression |
| title_short | TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 Expression |
| title_sort | twik 1 bac gfp transgenic mice an animal model for twik 1 expression |
| topic | TWIK-1 bacterial artificial chromosome transgenic mouse dentate gyrus lateral entorhinal cortex cerebellum |
| url | https://www.mdpi.com/2073-4409/10/10/2751 |
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