SALAD: Syringe-based Arduino-operated Low-cost Antibody Dispenser
Lateral Flow Assays (LFA) have been one of the most widely adopted technologies in clinical diagnosis over recent years, especially during the COVID-19 pandemic, due to their feasibility, compactness, and rapid readout. However, the precise dispensing of antibodies–a key part of the fabrication proc...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | HardwareX |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468067223000627 |
| _version_ | 1797676640932724736 |
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| author | Anh Phuc Hoang Le Quang Lam Nguyen Bao Hoai Pham Thien Hoang Minh Cao Toi Van Vo Khon Huynh Huong Thi Thanh Ha |
| author_facet | Anh Phuc Hoang Le Quang Lam Nguyen Bao Hoai Pham Thien Hoang Minh Cao Toi Van Vo Khon Huynh Huong Thi Thanh Ha |
| author_sort | Anh Phuc Hoang Le |
| collection | DOAJ |
| description | Lateral Flow Assays (LFA) have been one of the most widely adopted technologies in clinical diagnosis over recent years, especially during the COVID-19 pandemic, due to their feasibility, compactness, and rapid readout. However, the precise dispensing of antibodies–a key part of the fabrication process–requires costly line dispenser equipment, which poses a challenge to researchers with limited budgets. This study aims to resolve this key issue by introducing a Syringe-based Arduino-operated Low-cost Antibody Dispenser (SALAD). By utilizing a microneedle, stepper motor-driven syringe pump, and conveyor belt, SALAD can form micro-droplets to create an even band of antibodies. Our evaluation results showed comparable performance between SALAD and a commercialized model – Claremont ALFRD, with SALAD exceeding in affordability and feasibility. SALAD yielded an even signal, uniform bandwidth, and low background noise, yet optimization in the conveyor belt should be considered to enhance stability. With a low manufacturing cost ($200.61) compared to the commercialized models, our model is expected to provide an affordable approach for LFA researchers. |
| first_indexed | 2024-03-11T22:32:11Z |
| format | Article |
| id | doaj.art-b109d3eb372e42d49f52222de14408ed |
| institution | Directory Open Access Journal |
| issn | 2468-0672 |
| language | English |
| last_indexed | 2024-03-11T22:32:11Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | HardwareX |
| spelling | doaj.art-b109d3eb372e42d49f52222de14408ed2023-09-23T05:11:51ZengElsevierHardwareX2468-06722023-09-0115e00455SALAD: Syringe-based Arduino-operated Low-cost Antibody DispenserAnh Phuc Hoang Le0Quang Lam Nguyen1Bao Hoai Pham2Thien Hoang Minh Cao3Toi Van Vo4Khon Huynh5Huong Thi Thanh Ha6School of Biomedical Engineering, International University, Ho Chi Minh City, Viet Nam; Vietnam National University, Ho Chi Minh City, Viet NamSchool of Biomedical Engineering, International University, Ho Chi Minh City, Viet Nam; Vietnam National University, Ho Chi Minh City, Viet NamSchool of Biomedical Engineering, International University, Ho Chi Minh City, Viet Nam; Vietnam National University, Ho Chi Minh City, Viet NamSchool of Biomedical Engineering, International University, Ho Chi Minh City, Viet Nam; Vietnam National University, Ho Chi Minh City, Viet NamSchool of Biomedical Engineering, International University, Ho Chi Minh City, Viet Nam; Vietnam National University, Ho Chi Minh City, Viet NamSchool of Biomedical Engineering, International University, Ho Chi Minh City, Viet Nam; Vietnam National University, Ho Chi Minh City, Viet Nam; Corresponding authors at: School of Biomedical Engineering, International University, Ho Chi Minh City, Viet Nam.School of Biomedical Engineering, International University, Ho Chi Minh City, Viet Nam; Vietnam National University, Ho Chi Minh City, Viet Nam; Corresponding authors at: School of Biomedical Engineering, International University, Ho Chi Minh City, Viet Nam.Lateral Flow Assays (LFA) have been one of the most widely adopted technologies in clinical diagnosis over recent years, especially during the COVID-19 pandemic, due to their feasibility, compactness, and rapid readout. However, the precise dispensing of antibodies–a key part of the fabrication process–requires costly line dispenser equipment, which poses a challenge to researchers with limited budgets. This study aims to resolve this key issue by introducing a Syringe-based Arduino-operated Low-cost Antibody Dispenser (SALAD). By utilizing a microneedle, stepper motor-driven syringe pump, and conveyor belt, SALAD can form micro-droplets to create an even band of antibodies. Our evaluation results showed comparable performance between SALAD and a commercialized model – Claremont ALFRD, with SALAD exceeding in affordability and feasibility. SALAD yielded an even signal, uniform bandwidth, and low background noise, yet optimization in the conveyor belt should be considered to enhance stability. With a low manufacturing cost ($200.61) compared to the commercialized models, our model is expected to provide an affordable approach for LFA researchers.http://www.sciencedirect.com/science/article/pii/S2468067223000627ArduinoLateral Flow AssaysDispensersAntibodyPoint-of-careLow-cost |
| spellingShingle | Anh Phuc Hoang Le Quang Lam Nguyen Bao Hoai Pham Thien Hoang Minh Cao Toi Van Vo Khon Huynh Huong Thi Thanh Ha SALAD: Syringe-based Arduino-operated Low-cost Antibody Dispenser HardwareX Arduino Lateral Flow Assays Dispensers Antibody Point-of-care Low-cost |
| title | SALAD: Syringe-based Arduino-operated Low-cost Antibody Dispenser |
| title_full | SALAD: Syringe-based Arduino-operated Low-cost Antibody Dispenser |
| title_fullStr | SALAD: Syringe-based Arduino-operated Low-cost Antibody Dispenser |
| title_full_unstemmed | SALAD: Syringe-based Arduino-operated Low-cost Antibody Dispenser |
| title_short | SALAD: Syringe-based Arduino-operated Low-cost Antibody Dispenser |
| title_sort | salad syringe based arduino operated low cost antibody dispenser |
| topic | Arduino Lateral Flow Assays Dispensers Antibody Point-of-care Low-cost |
| url | http://www.sciencedirect.com/science/article/pii/S2468067223000627 |
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